Difference in somatic embryogenetic ability of cultured leaf explants of four genotypes of Solanum melongena L

Rao, PV Lakshmana

doi:10.1051/agro:19920605

Cited by 10 publications

(7 citation statements)

References 8 publications

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“…Such genotypic differences have been reported earlier in S. melongena (Yamada et al, 1967;Matsuoka and Hinata, 1979;Alicchio et al, 1982;Ali et al, 1991;Rao, 1992;Sharma and Rajam, 1995;Afele et al, 1996) and in several other crop plants (Bashkaran and Smith, 1990;Ezura et al, 1993;Ochoa Alejo and Ireta-Moreno, 1990;Pundeva and Simeonova, 1992;Tan et al, 1987).…”

Section: Effect Of Bapsupporting

confidence: 78%

“…In S. melongena, regeneration from the cotyledonary explants at different developmental stages ranging from one week old to 30 days old have been reported (Alicchio et al, 1982;Fobert and Webb, 1988;Rao, 1992;Saito and Nishimura, 1994;Momiyama et al, 1995;Sharma and Rajam, 1995;Hitomi et al, 1998;Magioli et al, 1998;Scoccianti et al, 2000;Tarre et al, 2004;Rahaman et al, 2006). Rama Swami et al (2005) reported in vitro response of cotyledons from three week old seedlings of S. surattense.…”

Section: Discussionmentioning

confidence: 99%

“…Although the genotypic variations have been observed earlier in this species with regard to the both direct and indirect organogenesis (Matsuoka and Hinata, 1979), no systematic study has been under taken to understand the possible reasons for the variations. However, Rao (1992) Sharma and Rajam (1995) demonstrated the effect of genotype, explants and its position on organogenesis and somatic embryogenesis in eggplant, it has confined to only a few cultivars of S. molongena L.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Genotype, Explant and Hormonal Concentration on in Vitro Response of Eggplant

Chakravarthi¹,

Indukuri²,

Goparaju³

et al. 2010

Not Sci Biol

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Three wild relatives Solanum: S. trilobatum L. (P1), S. indicum L. (P2), S. surattense Burm F. (P3) and four cultivars of S. melongena L.: 'Pusa Kranthi' (P4), 'Pusa purple long' (P5), 'Pusa purple cluster' (P6) and 'Green round' (P7) were evaluated for their callus induction frequency, total callus quantity, embryogenic callus quantity and plant regeneration ability. The present experiment revealed that in vitro response was significantly affected by the genotype, hormonal concentration and age of the explants (P<0.05). Cotyledon at walking stick stage of explants responded more than the opened leaves stage in all genotypes except in P1. Out of seven genotypes, P3 exhibited the highest callus induction, E-callus and regeneration response followed by P5 while highest total callus quantity obtained in P4. MS medium supplemented with 2 mg/l NAA+2.5 mg/l BAP was optimum for callus initiation, total callus quantity and embryogenic callus formation, and MS medium supplemented with 2.5 mg/l each BAP and KN were optimum for regeneration. Rooting of shoots occurred on half strength MS medium supplemented with 1 and 1.5 mg/l IBA respectively. The rooted plant lets were well accomplished with a survival frequency of 85.5±5%. Moreover, there were no phenotypic differences observed between the in vitro regenerated and in vivo plantlets.

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Section: Effect Of Bapsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Genotype, Explant and Hormonal Concentration on in Vitro Response of Eggplant

Chakravarthi¹,

Indukuri²,

Goparaju³

et al. 2010

Not Sci Biol

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“…insanum, Solanum gilo, Solanum integrifolium and their F1 hybrids, cultivars, and inbred lines (Alicchio et al, 1982;Gleddie et al, 1983;Ali et al, 1991;Rao, 1992;Anisuzzaman et al, 1993;Huda et al, 2007;Mir et al, 2008, Zayova et al, 2008Chakravarthi et al, 2010;Kaur et al, 2011a and. The differential responses for regeneration of adventitious shoots and somatic embryos, number of days to shoot initiation and mean number of Sidhu et al 715 shoots per callus (Sharma and Rajam, 1995a;Dobariya and Kachhadiya, 2004) are also there among cultivars.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

“…Generally, higher level of auxins and lower of cyto-kinine favours somatic embryogenesis. MS / LS medium supplemented with combination of 10 mgL -1 2, 4-dichloro-phenoxyacetic acid and 1 mgL -1 kinetin (Reynolds, 1986), 2ip (γγ-isopentyladenine) and IAA (Fassuliotis, 1975), 8 mgL -1 NAA and 0.1 mgL -1 Kin (Rao andSingh, 1991, Swamynathan et al, 2010), Zeatin @ 2 mgL -1 and NAA @ 0.01 mgL -1 (Fari et al, 1995), 1 mgL -1 NAA and 2 mgL -1 BAP (Salih and Al-Mallah, 2000), NAA or IBA at 0.5 mgL -1 (Anwar et al, 2002), 6-BA+ ZT (Zeatin) and 6-BA+IAA or ZT+ IAA Li et al, 2003) NAA+2.5 mgL -1 BAP (Rahman et al, 2006;Huda et al, 2007;Hossain et al, 2007;Zayova et al, 2008;Chakravarthi et al, 2010) induced the callus in eggplant (Table 1).…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Plant regeneration in eggplant (Solanum melongena L.): A review

Sidhu¹,

Dhatt²,

Sidhu³

2014

Afr. J. Biotechnol.

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Eggplant is highly responsive to various tissue culture techniques. Somatic embryogenesis and direct organogenesis are widely studied protocols in this crop, but potential of regeneration varies with genotype, explant and culture media supplemented with different combination and concentration of growth hormones. The genotype is the most important factor affecting somatic embryogenesis and organogenesis. Embryogenic competence occurs even within explant segments. Among growth regulators, auxins and cytokinins are of more significance as their ratio determines callogenesis, rhizogenesis, embryogenesis and regeneration in eggplant. Organogenesis and somatic embryogenesis related gene expression has been studied and transcripts have been analyzed through molecular studies. Efficient plant regeneration protocols would make a platform for exploitation of useful somaclonal variations, mutation breeding, induction of di-haploids, and genetic transformation with economically important genes for the improvement of eggplant.

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Eggplant

Rajam

Rotino²,

Sihachakr

et al. 2008

Compendium of Transgenic Crop Plants

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Eggplant ( Solanum melongena L.) is one of the important vegetable crops cultivated in various tropical and temperate parts of the world. There is a wide genetic diversity in the cultivated as well as wild species of eggplant. Although, the cultivated eggplant varieties are susceptible to various diseases and pests as well as abiotic stresses, the wild relatives of eggplant are good source of resistance against biotic and abiotic stresses. Therefore, attempts have been made to introgress these agronomically important traits from wild species to cultivated varieties of eggplant, but with limited success. Nevertheless, eggplant is quite amenable for plant regeneration through cell and tissue culture. Haploidisation, somatic hybridization and gene transfer methods have been well established for this crop. The beneficial traits like resistance to bacterial and fungal wilts have successfully been transferred from wild species to cultivated eggplants by protoplast fusion. However, the development of molecular markers and transgenic eggplants with novel traits is in infancy. Eggplant transgenics for insect resistance, abiotic stress tolerance, and parthenocarpy have been accomplished. More work is needed in this direction to produce eggplant transgenics for other agronomic traits, including disease resistance, nematode resistance, quality and shelf‐life of fruits. Modern biotechnological tools like RNA interference (RNAi) are useful to achieve these goals. The initiation of complete genome sequencing of eggplant is required to discover several useful genes, which can be introduced into eggplant and other crop species for genetic improvement. This chapter reviews the various aspects of eggplant biology, particularly the developments in eggplant biotechnology and future projections.

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Difference in somatic embryogenetic ability of cultured leaf explants of four genotypes of Solanum melongena L

Cited by 10 publications

References 8 publications

Effect of Genotype, Explant and Hormonal Concentration on in Vitro Response of Eggplant

Effect of Genotype, Explant and Hormonal Concentration on in Vitro Response of Eggplant

Plant regeneration in eggplant (Solanum melongena L.): A review

Eggplant

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