Differences in Acute and Convalescent-Phase Antibodies of Cats Infected with Feline Picornaviruses

Olsen, Richard G.; Kahn, Donald E.; Hoover, Edward A.; Saxe, Nancy J.; Yohn, David S.

doi:10.1128/iai.10.2.375-380.1974

Cited by 7 publications

(4 citation statements)

References 11 publications

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“…agreement with the finding of Olsen et al (11) for FCV-F9. The response was particularly marked with the conventional cats infected…”

Section: -supporting

confidence: 94%

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Cross-protection among feline caliciviruses

Povey¹,

Ingersoll²

1975

Infect Immun

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Each of five groups of specific-pathogen-free and conventionally reared cats was infected with a different strain of feline calicivirus. Two of the strains were pathogenic, producing characteristically fever, depression, loss of appetite, buccal ulceration, and occasionally increased ocular and nasal secretion. Two of the other strains were mildly pathogenic and associated with fever or buccal ulceration or both; the fifth strain was nonpathogenic. The two pathogenic strains plus three others shown also to be pathogenic were used 3 months after

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“…agreement with the finding of Olsen et al (11) for FCV-F9. The response was particularly marked with the conventional cats infected…”

Section: -supporting

confidence: 94%

“…4. Geometric mean serum neutralizing anti-Olsen et al (11), working with two caliciviagainst challenge virus in (A) conventional and ruses, found that serum neutralizing antibody 'PF cat groups, before and after challenge.…”

Section: -mentioning

confidence: 99%

Cross-protection among feline caliciviruses

Povey¹,

Ingersoll²

1975

Infect Immun

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“…The protective effect of the FCV F9 vaccination to induce seroconversion and to reduce severe clinical signs was demonstrated in studies from the 1970s–90s using different protocols of vaccination and challenge [ 14 , 15 , 16 , 34 , 35 ]. The challenge infection by aerosolisation, used in most of these studies, did not reflect the natural method of infection and clinical signs were often located in the lower respiratory tract.…”

Section: Discussionmentioning

confidence: 99%

Modified-Live Feline Calicivirus Vaccination Reduces Viral RNA Loads, Duration of RNAemia, and the Severity of Clinical Signs after Heterologous Feline Calicivirus Challenge

Spiri

Riond

Stirn

et al. 2021

Viruses

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Feline calicivirus (FCV) is a common cat virus causing clinical signs such as oral ulcerations, fever, reduced general condition, pneumonia, limping and occasionally virulent-systemic disease. Efficacious FCV vaccines protect against severe disease but not against infection. FCV is a highly mutagenic RNA virus whose high genetic diversity poses a challenge in vaccine design. The use of only one modified-live FCV strain over several decades might have driven the viral evolution towards more vaccine-resistant variants. The present study investigated the clinical signs, duration, and amount of FCV shedding, RNAemia, haematological changes and acute phase protein reaction in SPF cats after subcutaneous modified-live single strain FCV vaccination or placebo injection and two subsequent oronasal heterologous FCV challenge infections with two different field strains. Neither clinical signs nor FCV shedding from the oropharynx and FCV RNAemia were detected after vaccination. After the first experimental infection, vaccinated cats had significantly lower clinical scores, less increased body temperature and lower acute phase protein levels than control cats. The viral RNA loads from the oropharynx and duration and amount of RNAemia were significantly lower in the vaccinated animals. No clinical signs were observed in any of the cats after the second experimental infection. In conclusion, FCV vaccination was beneficial for protecting cats from severe clinical signs, reducing viral loads and inflammation after FCV challenge.

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“…The levels of such VN antibodies correlate well with protection against homologous challenge (39). There is also production of immunoglobulin G (IgG)-and IgA-associated mucosal immunity (25) and serum immunofluorescence, complement fixation, complement fixation inhibition, and agar gel precipitation antibodies (15,34,57). Although major histocompatibility complex-restricted cytotoxic activity of peripheral blood T lymphocytes has been demonstrated in vaccinated cats, the significance of cytotoxic T lymphocytes to FCV protection is not known (51).…”

mentioning

confidence: 99%

The Capsid Gene of Feline Calicivirus Contains Linear B-Cell Epitopes in both Variable and Conserved Regions

Radford¹,

Willoughby²,

Dawson

et al. 1999

J Virol

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In order to map linear B-cell (LBC) epitopes in the major capsid protein of feline calicivirus (FCV), an expression library containing random, short (100- to 200-bp) fragments of the FCV F9 capsid gene was constructed. Analysis of this library showed it to be representative of the region of the capsid gene that encodes the mature capsid protein. The library was screened by using polyclonal antisera from a cat that had been challenged experimentally with F9 to identify immunoreactive clones containing LBC epitopes. Twenty-six clones that reacted positively to feline antisera in immunoblots were identified. FCV-derived sequence from these clones mapped to a region of the capsid that spanned 126 amino acids and included variable regions C and E. An overlapping set of biotinylated peptides corresponding to this region was used to further map LBC epitopes by using F9 antisera. Four principal regions of reactivity were identified. Two fell within the hypervariable region at the 5′ end of region E (amino acids [aa] 445 to 451 [antigenic site {ags} 2] and aa 451 to 457 [ags 3]). However, the other two were in conserved regions (aa 415 to 421 [ags 1; region D] and aa 475 to 479 [ags 4; central region E]). The reactivity of the peptide set with antisera from 11 other cats infected with a range of FCV isolates was also determined. Ten of 11 antisera reacted to conserved ags 4, suggesting that this region may be useful for future recombinant vaccine design.

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Differences in Acute and Convalescent-Phase Antibodies of Cats Infected with Feline Picornaviruses

Cited by 7 publications

References 11 publications

Cross-protection among feline caliciviruses

Cross-protection among feline caliciviruses

Modified-Live Feline Calicivirus Vaccination Reduces Viral RNA Loads, Duration of RNAemia, and the Severity of Clinical Signs after Heterologous Feline Calicivirus Challenge

The Capsid Gene of Feline Calicivirus Contains Linear B-Cell Epitopes in both Variable and Conserved Regions

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