Differences in microbial diversity and environmental factors in ploughing-treated tobacco soil

Abstract

During agricultural production, plowing affects the existing traits of the planted soil, including environmental factors (physicochemical properties and soil enzymatic activity) and microbial community, but whether deep tillage and conventional tillage cause differences in soil microecology are unknown. In this study, the 16S rRNA high-throughput sequencing technology was combined with soil environmental factor detection to analyze the differences in microbial diversity of smokey soils at different depths. As a result, the composition and structure of microbial community varied in different soil depth after plowing. Two dominant phyla, Actinobacteria and Acidobacteria, have varied a lot between the deep-plowing treatment HS3 (the sample in 10–20 cm depth after deep-plowing treatment) sample and the conventional tillage HC3 (treatment the sample in 10–20 cm depth after conventional tillage) sample. The abundance of Actinobacteria has increased significantly, while the abundance of Acidobacteria has decreased significantly. Moreover, deep tillage increased the activity of sucrase (S-SC) and nitrate reductase (NR) in samples with soil depth below 20 cm. In summary, deep tillage disturbed spatial microbial diversity and environmental factors significantly. This would provide new guidance for improving farmland management strategies, optimizing the activation methods of soil layers, further improving crop planting soil, and increasing crop yield.

Revealing the role of the rhizosphere microbiota in reproductive growth for fruit productivity when inorganic fertilizer is partially replaced by organic fertilizer in pear orchard fields

“…Elusimicrobia was unique to the rare group of bacteria. Zhao et al ( 2021 ) and Zhang et al ( 2022 ), reported that Firmicutes and Entotheonellaeota show a high correlation with soil nutrient content, while rare groups of Elusimicrobia in bacterial communities had no correlation with soil nutrient content. It seems clear that the input of organic fertilizer has increased the portion of class Clostridia, which belongs to Firmicutes and may degrade the lignin and cellulose.…”

Characterizing bacterial communities in Phragmites australis rhizosphere and non-rhizosphere sediments under pressure of antibiotics in a shallow lake

“…The concentrations of nine antibiotics [norfloxacin (NOR), ofloxacin (OFL) and ciprofloxacin (CIP), oxytetracycline (OTC), tetracycline (TC), sulfapyridine (SPD) and sulfadiazine (SDZ), erythromycin (ERM) and roxithromycin (ROM)] was extracted and determinated according to our previous study ( Zhang et al, 2022 ; Supplementary Table S1 ). Briefly, the antibiotics in the extract were determined by liquid chromatography-mass spectrometry (LC-ESI-MS/MS) in multiple reactive ion detection mode (MRM).…”

Nitric Acid Rain Decreases Soil Bacterial Diversity and Alters Bacterial Community Structure in Farmland Soils

“…Soil microorganisms are an important part of the ecosystem and play an important role in maintaining soil ecological functions [7,8]. Microbial community structure can reflect the quality and health of soil to a certain extent, which is of great value in exploring the functional characteristics of soil and the structural properties of the ecosystem [9]. Previous studies have shown that AR can lead to soil acidification [10][11][12], which can promote an increase in the concentration of hydrogen ions in soil and cause changes in the composition and structure of soil microbial communities [13].…”