Different antioxidant defense responses to salt stress during germination and vegetative stages of endemic halophyte Gypsophila oblanceolata Bark.

Sekmen, A. Hediye; Türkan, İsmail; Tanyolaç, Zehra Özgecan; Ozfidan, Ceyda; Dinç, Ahmet

doi:10.1016/j.envexpbot.2011.10.012

Cited by 116 publications

(65 citation statements)

References 92 publications

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“…El estrés osmótico provocado por un medio rico en sales podría hacer que el metabolismo de los tejidos vegetales estimule la liberación de compuestos que son fáciles de oxidar convirtiéndose en fitotóxicos (Turkan y Demiral 2009). La inducción de síntesis de enzimas antioxidantes como catalasa, peroxidasa y superoxido dismutasa causada por estrés salino durante la germinación ha sido reportada por Sekmen et al (2012).…”

Section: Discussionunclassified

Efecto de la concentración y de los componentes del medio de cultivo MS sobre la germinación in vitro de Ugni molinae

2014

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SUMMARYThe objective of the study was to evaluate the effect of concentration and components of the Murashige and Skoog (MS) medium on in vitro germination of Ugni molinae. Two tests were performed; in the first one, different concentrations of the MS medium were used (full-strength MS, ½ MS, ¼ MS, ⅛ MS and MS free). In the second experiment, one of the component was excluded (macro salts, micro salts or vitamins). A tetrazolium test was performed to assess viability. After the first two weeks of sowing, all treatments started germination, which was ascending until approximately 20 days, and then a slowdown was observed, which remained stable between days 40 and 60. All treatments containing any concentration of MS presented lower germination than that presented by the MS free medium, as the media containing some of its components, especially macro salts. The tetrazolium test showed 87 % of viability of the seeds: the maximum germination was 42%, which showed a strong latency. Germination of U. molinae is affected by the presence of the MS medium salts. Based on these results the use of only water and agar are recommended as the in vitro germination medium.Key words: Myrtaceae, tissue culture, MS medium, murtilla, viability. RESUMENEl objetivo de este estudio fue evaluar el efecto de la concentración y los componentes del medio Murashige y Skoog (MS) sobre la germinación in vitro de Ugni molinae. Para ello se realizaron dos ensayos, en el primero, se utilizaron distintas concentraciones del medio MS (MS completo, ½ MS, ¼ MS, ⅛ MS y sin MS) y, en el segundo, se excluyó uno de sus componentes (macrosales, microsales o vitaminas). La viabilidad se determinó mediante la prueba de tetrazolio. Después de las dos primeras semanas de siembra, todos los tratamientos iniciaron la germinación, la que fue en aumento hasta aproximadamente los 20 días, luego se observó una desaceleración hasta mantenerse estable entre los 40 a 60 días. Todos los medios de cultivo que contenían alguna concentración de MS obtuvieron una germinación más baja que el medio sin MS, como también los medios que contenían algunos de sus componentes, especialmente macrosales. La prueba de tetrazolio determinó un 87 % de viabilidad en las semillas en tanto que la germinación máxima alcanzada fue de 42 % lo que evidenció una fuerte latencia. La germinación de U. molinae es muy sensible a la presencia de sales del medio MS razón por la cual se aconseja solo el uso de agua y agar como medio de germinación in vitro.Palabras clave: mirtáceas, cultivo de tejidos, medio MS, murtilla, viabilidad. INTRODUCCIÓNLa murtilla (Ugni molinae Turcz.) es un arbusto endé-mico de Chile, perteneciente a la familia de las mirtáceas. La planta posee tanto un sistema de propagación sexual como vegetativo lo que le proporciona una gran flexibilidad a su establecimiento y perpetuación. Las semillas se diseminan en forma endozoica, en ambiente natural germina después de cinco semanas entre los meses de octubre a diciembre y su germinación es baja respecto a otras mirtáceas (Figue...

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Section: Discussionunclassified

Efecto de la concentración y de los componentes del medio de cultivo MS sobre la germinación in vitro de Ugni molinae

2014

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“…1). Under stressful conditions (such as salinity stress), osmoprotection of membranes with the accumulation of osmolytes is a likely mechanism of plant survival (Sekmen et al 2012). The maintenance of steady state level of FP and increased accumulation of GB and SS in this study suggest that Pro and GB pretreatment strategy adopted here was quite effective in partially repairing the salinity damage to sugarcane buds.…”

Section: Discussionmentioning

confidence: 65%

“…The bud tissue was deeply examined for changes in the level of H 2 O 2 production, GB, FP, and SS levels, since their accumulation has been associated with salinity tolerance of the number of plant species (Yang and Lu 2005;Sekmen et al 2012), but comparative information is scarce for sugarcane buds subjected to salinity stress. In this study, a high level of H 2 O 2 production in the salinity-stressed buds and its reduction more with GB than Pro at different time intervals (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The development of various tissues in the embryo (in case of seeds) and shoot apices (in case of nodes or buds) is due to the expression of these genes. Although, the production of shoot from propagules, including nodal or other vegetative buds, leads eventually to the production of shoots, although it is entirely different from those of seeds Sekmen et al 2012). The sequence of mechanisms governing histological differentiation from immature nodal buds up to the production of shoot in different plant species, especially using pretreatment strategies, has not been studied well and needs thorough investigations.…”

Section: Introductionmentioning

confidence: 99%

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Partial repair of salinity-induced damage to sprouting sugarcane buds by proline and glycinebetaine pretreatment

et al. 2015

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Sugarcane shows reduced crop stand under relatively suboptimal conditions; the main reason for this is its sensitivity to ionic stress in the soil solution. This research was performed to explore some physiological and developmental changes in the immature sugarcane buds submitted to salt stress and possible role of glycinebetaine (GB) and proline (Pro) in mitigating the ion toxicity in a time course manner. Salinity stress reduced fresh and dry weight, induced the generation of hydrogen peroxide, increased tissue levels of Na(+) sand Cl(-), reduced K(+) and Ca(2+), and K(+):Na(+) and Ca(2+):Na(+) ratios, while increasing the osmolyte synthesis in expanding sugarcane buds. Salinity stress reduced and delayed the formation of new bud leaves and their expansion, which was mainly because of reduction in the number and area of mesophyll cells and poor development of vascular bundles. The pretreatment of bud chips with 20 mM each of GB and Pro decreased tissue levels of Na(+) and Cl(-), reduced the generation of H2O2, improved K(+) and Ca(2+), K(+):Na(+) and Ca(2+):Na(+) ratios, and further increased the levels of GB, free proline (FP), and soluble sugars in the buds. The pretreatment increased mesophyll cell number and expansion of bud leaves and formation of elaborated vascular tissues, which apparently enabled the sprouting buds to adapt to salinity stress. Of the two osmolytes, GB was a relatively better inducer of salinity tolerance than Pro. In short, salinity-induced oxidative stress was the main cause for altered tissue development, the production of which was offset by pretreatment of bud tissues with Pro and GB.

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“…This idea is further supported by accumulating higher concentration of proline in sensitive rice cultivars than in tolerant genotypes under salt stress (Lutts et al 1999). However, significant roles of proline involved in salt adaptation or salt tolerance have been demonstrated in some plants and cell cultures (Banu et al 2009;Lokhande et al 2010;Sekmen et al 2012). A series of experiments provide the important evidence that the accumulation of proline can be used as a biochemical marker for the increase of salt tolerance in transgenic plants (Gleeson et al 2005;Chen et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Signal regulation of proline metabolism in callus of the halophyte Nitraria tangutorum Bobr. grown under salinity stress

Yang

et al. 2012

Plant Cell Tiss Organ Cult

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Different antioxidant defense responses to salt stress during germination and vegetative stages of endemic halophyte Gypsophila oblanceolata Bark.

Cited by 116 publications

References 92 publications

Efecto de la concentración y de los componentes del medio de cultivo MS sobre la germinación in vitro de Ugni molinae

Efecto de la concentración y de los componentes del medio de cultivo MS sobre la germinación in vitro de Ugni molinae

Partial repair of salinity-induced damage to sprouting sugarcane buds by proline and glycinebetaine pretreatment

Signal regulation of proline metabolism in callus of the halophyte Nitraria tangutorum Bobr. grown under salinity stress

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