Different Approaches in Manipulating Ratio Spectra for Analyzing Amlodipine Besylate and Irbesartan Combination

Abstract

Background Hypertension is a key risk factor for ischemic heart disease and atherosclerosis. Most patients require a combination of antihypertensive medications to accomplish their therapeutic goals. Antihypertensive medicines such as calcium channel blockers and angiotensin receptor blockers are indicated for patients whose high blood pressure cannot be controlled with monotherapy. The combination of amlodipine besylate (AML) with irbesartan (IRB) is an example of this synergistic activity in lowering blood pressure. Objective In this regard, the goal of the research is to develop sensitive spectrophotometric methods for the simultaneous determination of amlodipine besylate and irbesartan. Methods Three simple ratio spectra-manipulating spectrophotometric methods namely, ratio difference, mean centering of ratio spectra, and derivative ratio, were developed for the simultaneous assay of the cited mixture. Results Linear correlations were attained over the concentration range of 1–35 μg/mL and 2–35 μg/mL for amlodipine besylate and irbesartan, respectively. The methods were validated according to the International Conference on Harmonization guidelines with good results. Conclusion The methods developed were successfully applied for the assay of the cited drugs in their marketed formulation. They could be efficiently used for routine analysis of the mentioned drugs in QC laboratories. Highlights The proposed approaches do not require expensive solvents or complex instruments. They could be used in routine laboratory tests where time and cost are crucial.

Synthesis of Prussian Blue Analogue and Its Catalytic Activity toward Reduction of Environmentally Toxic Nitroaromatic Pollutants

“…The reduction process was monitored by UV–vis spectrophotometry by scanning at definite intervals. 51 It was found that the peak of 4-NA gradually decreased at 380 nm with time and completely vanished after 270 s as shown in Figure 5 . Furthermore, a new peak corresponding to p -phenylenediamine ( p -PDA) was simultaneously observed at 240 nm.…”

“…The catalytic activity of PBA was investigated by reducing 4-NA to utilize NaBH 4 as a reducing agent in aqueous media as presented in Figure . The reduction process was monitored by UV–vis spectrophotometry by scanning at definite intervals . It was found that the peak of 4-NA gradually decreased at 380 nm with time and completely vanished after 270 s as shown in Figure .…”

Rapid Identification of Phenolics in Mixtures by Two‐Dimensional Tandem Mass Spectrometry with Microdroplet Accelerated Derivatization Reactions

“…To test the generalizability of the NL values found, a total of 23 different phenolic compounds was investigated and the data are tabulated in Table 1 (all the 2D MS/MS subtracted spectrum can be found in the Figure S4). The table includes phenols significance in industrial production, environmental research, as inhibitors, therapeutic drugs, as brain metabolites, and as starting materials in synthesis of industrial, agricultural, or pharmaceutical compounds [30–38] . The phenolic compounds covered in this study showed moderate to high reactivity with PTAD.…”

Utilization of Localized Surface Plasmon Resonance of Silver Nanoparticles for ‎the Spectrophotometric Estimation of Amlodipine and Hydrochlorothiazide

“…An electroanalytical method based on a chitosan-Prussian blue nanocomposite membrane potentiometric sensor was developed to determine amlodipine selectively 12 . Additional spectrophotometric methods for assessing AM in combination with other drugs have been published and proposed [13][14][15][16] .…”