Different contribution of BRINP3 gene in chronic periodontitis and peri-implantitis: a cross-sectional study

Abstract

BackgroundPeri-implantitis is a chronic inflammation, resulting in loss of supporting bone around implants. Chronic periodontitis is a risk indicator for implant failure. Both diseases have a common etiology regarding inflammatory destructive response. BRINP3 gene is associated with aggressive periodontitis. However, is still unclear if chronic periodontitis and peri-implantitis have the same genetic background.The aim of this work was to investigate the association between BRINP3 genetic variation (rs1342913 and rs1935881) and expression and susceptibility to both diseases.MethodsPeriodontal and peri-implant examinations were performed in 215 subjects, divided into: healthy (without chronic periodontitis and peri-implantitis, n = 93); diseased (with chronic periodontitis and peri-implantitis, n = 52); chronic periodontitis only (n = 36), and peri-implantitis only (n = 34). A replication sample of 92 subjects who lost implants and 185 subjects successfully treated with implants were tested. DNA was extracted from buccal cells. Two genetic markers of BRINP3 (rs1342913 and rs1935881) were genotyped using TaqMan chemistry. Chi-square (p < 0.05) compared genotype and allele frequency between groups. A subset of subjects (n = 31) had gingival biopsies harvested. The BRINP3 mRNA levels were studied by CT method (2ΔΔCT). Mann–Whitney test correlated the levels of BRINP3 in each group (p < 0.05).ResultsStatistically significant association between BRINP3 rs1342913 and peri-implantitis was found in both studied groups (p = 0.04). The levels of BRINP3 mRNA were significantly higher in diseased subjects compared to healthy individuals (p = 0.01).ConclusionThis study provides evidence that the BRINP3 polymorphic variant rs1342913 and low level of BRINP3 expression are associated with peri-implantitis, independently from the presence of chronic periodontitis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12903-015-0018-6) contains supplementary material, which is available to authorized users.

Matrix metalloproteinases gene polymorphism haplotype is a risk factor to implant loss: A case‐control study

“…The literature supports the evidence that SNPs play an important role in the transcription of these inflammatory mediators and may influence the osseointegration success, including single‐nucleotide polymorphism (SNPs) in MMPs …”

“…20 Moreover, an intense inflammatory process is mediated by cytokines that point to different cell types and stimulating the production of prostaglandins and MMPs, which are associated with bone and connective tissue breakdown. 21 The literature supports the evidence that SNPs play an important role in the transcription of these inflammatory mediators and may influence the osseointegration success, [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] including singlenucleotide polymorphism (SNPs) in MMPs. [8][9][10] An important point to validate the association between polymorphism and pathological processes is a methodological and study design restricted.…”

“…Some single‐nucleotide polymorphism (SNPs) may affect gene expression levels and protein production or functions; consequently, they influence osteogenesis and inflammatory responses. Some SNPs in cytokines and lipid mediators genes were associated with implant loss and peri‐implantitis, explain some of interindividual risk factors …”

Dental genetics in Brazil: Where we are

“… There is high level of RANKL/OPG in peri‐implant mucosae of patients with history of chronic periodontitis (Costa et al., ). Haplotypes in BMP4 and FGF genes increase the risk of peri‐implantitis (Coelho et al., ) MMP13 , TIMP2, and TGFB3 gene polymorphisms are associated with Brazilian chronic periodontitis and peri‐implantitis (Junior et al., ). BRINP3 gene has different contribution in chronic periodontitis and peri‐implantitis (Casado et al., ). IL6 gene polymorphism is associated with periodontitis and peri‐implantitis in a Brazilian population (Casado et al., ,b). …”

Genetics of Periodontitis without Bias

“…11 Targeted fine-mapping work identified BRINP3, in 1q31.3, as associated with aggressive periodontitis, 12 and later, this result was replicated in cohorts with chronic periodontitis and peri-implantitis. 13,14 Interestingly, the GWAS study that identified GLT6D1 as associated with aggressive periodontitis 2 showed P-values ranging from 0.0001 to 0.0003 for markers close to BRINP3 (Table 1). This scenario is likely an example that suggests that it may be an oversimplification to just consider GWAS as the main source of believable genetic signals for periodontitis.…”