Different expression of lipid metabolism-related genes in Shandong black cattle and Luxi cattle based on transcriptome analysis

Abstract

To provide new ideas for improving meat quality and generating new breeds of cattle, the important candidate genes affecting fat deposition in two kinds of cattle were identified. Eighteen months Shandong black cattle (n = 3) and Luxi cattle (n = 3) were randomly assigned into two environmental. The longissimus dorsi muscles of Shandong Black Cattle and Luxi Cattle were collected and analyzed by fatty acid determination, high-throughput sequencing transcriptomics, qRT-PCR expression profile and western blot. The ratio of unsaturated fatty acids to saturated fatty acids was 1.37:1 and 1.24:1 in the muscle tissues of Shandong black cattle and Luxi cattle, respectively. The results of RNA-Seq analysis revealed 1320 DEGs between the longissimus dorsi of Shandong black cattle and Luxi cattle. A total of 867 genes were upregulated, and the other 453 genes were downregulated. With GO enrichment analysis, it was found that the identified DEGs were significantly enriched in regulation of the Wnt signaling pathway, negative regulation of the Wnt signaling pathway, cAMP metabolic process, fat cell differentiation and among other functions. We found that regulation of lipolysis in adipocytes was the significant enrichment pathway of upregulated genes and downregulated genes, PPAR signaling pathway and AMPK signaling pathway are highly representative pathways of lipid metabolism in Shandong black cattle. Network analysis showed that PPARGC1A, ADCY4, ANKRD6, COL1A1, FABP4, ADIPOQ, PLIN1, PLIN2, and LIPE genes were correlated with key loci genes in multiple metabolic pathways. Meanwhile we found that FABP4 and ADIPOQ had 7 common regulatory factors in different genes, which were PLIN1, PLIN2, PPARGC1A, RXRA, PCK1, LEPR, LEP. These genes were involved in regulation of lipolysis in adipocytes, adipocytokine signaling pathway, PPAR signaling pathway. FABP4 and ADIPOQ were selected as important candidate marker genes for fat deposition based on the results.

Interference With ACSL1 Gene in Bovine Adipocytes: Transcriptome Profiling of mRNA and lncRNA Related to Unsaturated Fatty Acid Synthesis

“…Previous studies have confirmed the role of COL5A3, COL6A2 , and COL3A1 in inducing CD44 expression, followed by upregulation of the cellular expression of docosanoic acid, palmitic acid, and trans-oleic acid and downregulation of the cellular expression of tridecanoic acid, stearic acid, and cis-5, 8, 11, 14-EPA ( 79 ). Liu et al ( 26 ) found that COL6A1, COL6A2, COL4A2, COL1A1, COL4A6, COL4A5, COL6A3, COL1A2 , and other genes were strongly related to fat metabolism in the transcriptomic study of the muscles of Shandong black cattle and Luxi cattle.…”

“…Jiang et al ( 25 ) isolated 3,716 candidate lncRNAs from fat samples from calves and adult cattle and confirmed their crucial role in cattle of various ages. Liu et al ( 26 ) demonstrated that genes, such as PLIN1, PLIN2 , and PPARGC1A regulated various lipid-related pathways. Cai et al ( 31 ) identified a novel lncRNA, BADLNCR1 , that suppressed bovine adipogenesis by inhibiting GLRX5 expression.…”

RNA-Seq Reveals Function of Bta-miR-149-5p in the Regulation of Bovine Adipocyte Differentiation

“…The cAMP and calcium signaling pathways act as the second messengers in eukaryotic cells [80] and regulate the intracellular lipolysis in adipocytes through calcium ions and cAMP levels [81]. There is substantial evidence that MAPK sig-naling pathway [82,83], lipid metabolism, insulin signaling [84], AMPK signaling pathway, Wnt signaling pathway [85][86][87][88], and TGF-beta signaling pathway [89,90] perform crucial roles in adipogenesis. The results of the present study support the interactive roles of the signaling pathways in the regulation of adipogenesis.…”

A Study of the Regulatory Mechanism of the CB1/PPARγ2/PLIN1/HSL Pathway for Fat Metabolism in Cattle

“…Some high-frequency but unclear regulatory genes related to fat decomposition were found. We analyzed the metabolic pathways identified in the KEGG database and the complete transcriptome database established in our laboratory to speculate the possible regulatory mechanisms ( Liu et al, 2020 ). In this experiment, CB1 was selected to regulate HSL, which was an approach that had not been previously explored.…”

“…With the approval of the local government, we have established the local standard of Shandong black cattle. The major genes FABP4, MSTN and HSL related to fat deposition, muscle tenderness and marbling have been preliminarily screened ( Liu et al, 2020 ). As a molecular marker for early screening of cattle, ultrasonic was used to determine marbling, eye muscle area, back fat thickness and intramuscular fat content.…”