Different laccase detoxification strategies for ethanol production from lignocellulosic biomass by the thermotolerant yeast Kluyveromyces marxianus CECT 10875

Moreno, Antonio D.; Ibarra, David; Fernández, José Luis Fernández; Ballesteros, Mercedes

doi:10.1016/j.biortech.2011.11.108

Cited by 101 publications

(73 citation statements)

References 33 publications

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“…Kalyani et al [79] observed an enhancement in the saccharification yield by 48% of acid-pretreated rice straw due to a phenols reduction by C. perennis laccase. Contrary, Tabka et al [102], Jurado et al [76] and Moreno et al [80,89] described lower glucose concentration after enzymatic hydrolysis of steam-exploded wheat straw treated with P. cinnabarinus, T. villosa and C. rigida laccases. This negative phenomenon was attributed to the formation of laccase-derived compounds from phenols that inhibit cellulolytic enzymes.…”

Section: Detoxification and Saccharificationmentioning

confidence: 94%

“…Kalyani et al [79] achieved a phenol removal of 76% when steam-exploded whole slurry from rice straw was treated with Coltricia perennis laccase. Moreno et al [80] reported higher phenol reductions (93-95%) when Pycnoporus cinnabarinus and Trametes villosa laccases were used to detoxify steam-exploded wheat straw. Similar ranges were observed by Jönsson et al [81] with acid steam-pretreated willow and T. versicolor laccase, and by Jurado et al [76] with both water and acid-impregnated steam-exploded wheat straw and T. versicolor and C. rigida laccases.…”

Section: Detoxification Mechanismmentioning

confidence: 99%

“…Then, the treatment of steam-exploded wheat straw with a fungal laccase from T. villosa at optimal pH 4 removed 90% of phenols, while a reduction in the phenol content of 29% was achieved with a bacterial laccase from S. ipomoea at optimal pH 8 [95]. Regarding to temperature, Moreno et al [80] reported phenols reduction around of 94% when steam-exploded wheat straw was treated with laccases from P. cinnabarinus and T. villosa at their optimal temperatures of 50 and 30 • C, respectively. The treatment time and the enzyme loading at which the detoxification is carried out are also two important factors.…”

Section: Detoxification Mechanismmentioning

confidence: 99%

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Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

et al. 2017

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Abstract:The continuous increase in the world energy and chemicals demand requires the development of sustainable alternatives to non-renewable sources of energy. Biomass facilities and biorefineries represent interesting options to gradually replace the present industry based on fossil fuels. Lignocellulose is the most promising feedstock to be used in biorefineries. From a sugar platform perspective, a wide range of fuels and chemicals can be obtained via microbial fermentation processes, being ethanol the most significant lignocellulose-derived fuel. Before fermentation, lignocellulose must be pretreated to overcome its inherent recalcitrant structure and obtain the fermentable sugars. Usually, harsh conditions are required for pretreatment of lignocellulose, producing biomass degradation and releasing different compounds that are inhibitors of the hydrolytic enzymes and fermenting microorganisms. Moreover, the lignin polymer that remains in pretreated materials also affects biomass conversion by limiting the enzymatic hydrolysis. The use of laccases has been considered as a very powerful tool for delignification and detoxification of pretreated lignocellulosic materials, boosting subsequent saccharification and fermentation processes. This review compiles the latest studies about the application of laccases as useful and environmentally friendly delignification and detoxification technology, highlighting the main challenges and possible ways to make possible the integration of these enzymes in future lignocellulose-based industries.

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Section: Detoxification and Saccharificationmentioning

confidence: 94%

Section: Detoxification Mechanismmentioning

confidence: 99%

Section: Detoxification Mechanismmentioning

confidence: 99%

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Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

et al. 2017

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“…These includes low pH tolerance, high resistance to furfural and alcohols, a broad range of fermentation temperatures (thermotolerance), and the ability to grow fast and on a wide variety of inexpensive carbon source (Moreno et al 2012, Chang et al 2014, Galindo-Leva et al 2016. Some biotechnological applications have been reported using this yeast, such as food, beverages, enzymes, and fine chemicals production (Foukis et al 2012, Lane et al 2011.…”

Section: Introductionmentioning

confidence: 99%

Asymmetric bioreduction of β-ketoesters derivatives by Kluyveromyces marxianus: influence of molecular structure on the conversion and enantiomeric excess

Oliveira

Bello

Rodrigues

et al. 2017

An. Acad. Bras. Ciênc.

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This study presents the bioreduction of six β-ketoesters by whole cells of Kluyveromyces marxianus and molecular investigation of a series of 13 β-ketoesters by hologram quantitative structure-activity relationship (HQSAR) in order to relate with conversion and enantiomeric excess of β-stereogenichydroxyesters obtained by the same methodology. Four of these were obtained as (R)-configuration and two (S)-configuration, among them four compounds exhibited >99% enantiomeric excess. The β-ketoesters series LUMO maps showed that the β-carbon of the ketoester scaffold are exposed to undergo nucleophilic attack, suggesting a more favorable β-carbon side to enzymatic reduction based on adopted molecular conformation at the reaction moment. The HQSAR method was performed on the β-ketoesters derivatives separating them into those provided predominantly (R)-or (S)-β-hydroxyesters. The HQSAR models for both (R)-and (S)-configuration showed high predictive capacity. The HQSAR contribution maps suggest the importance of β-ketoesters scaffold as well as the substituents attached therein to asymmetric reduction, showing a possible influence of the ester group carbonyl position on the molecular conformation in the enzyme catalytic site, exposing a β-carbon side to the bioconversion to (S)-and (R)-enantiomers.

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“…They can also be used as animal feed, as materials for aerobic composting, or as resources for anaerobic digestion (Zhang et al, 2011); however, the reuse of these materials is not widespread, because of high costs or the Phomopsis liquidambari B3 can produce laccase and degrade lignin (Dai et al, 2010a;Chen et al, 2013;Dai et al, 2010b). Laccases (EC 1.10.3.2) are multicopper-containing oxidases (Moreno et al, 2012) that play roles in various biological processes, including fungal morphogenesis, pathogenesis, detoxification, lignification, and delignification (Camarero et al, 2012;Lee et al, 2012). Laccases have also been used in diverse biotechnological fields, including organic synthesis, bioremediation, beverage processing, and pulp/textile bleaching (Jeon et al, 2013;Rodríguez et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Laccase production by Phomopsis liquidambari B3 cultured with food waste and wheat straw as the main nitrogen and carbon sources

Zhou

Yang

Mei

et al. 2014

Journal of the Air & Waste Management Association

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Food waste is the most difficult waste to manage because of the high treatment costs and the risk of environmental contamination. Wheat straw burning has become an increasingly discussed topic in recent years because of the air pollution it causes. However, food waste and wheat straw contain high amounts of nutrient elements; efficient utilization of these wastes can improve the environment by lessening airborne pollutant emission and the amount of waste that must be landfilled. It is well known that use of low-cost and abundant waste materials in microbial fermentations can reduce product costs. We aimed to use these resources while improving laccase production by the endophytic fungus Phomopsis liquidambari B3. We cultured P. liquidambari B3 in medium containing food waste and wheat straw as the main nitrogen and carbon sources, respectively. We optimized the fermentation conditions by response surface methodology (RSM), using a Box-Behnken design for RSM I and a central composite design for RSM II. Optimization resulted in an 11.07-fold (1.98-fold RSM I; 5.59-fold RSM II) increase in laccase yield compared with that before optimization. The model was validated by mathematical evaluations and by comparisons between predicted and experimental values. Under optimized conditions, 53.76% of lignin in wheat straw was degraded. By optimizing fermentation conditions and using multiple bioresources, laccase production by this fungus was increased. These results provide the foundation for future research and for scaled-up laccase production.Implications: Food waste and wheat straw are waste products, but they could be bioresources if they were managed properly. In this work, we innovatively used a mixture of food waste and wheat straw as a substrate for the novel strain Phomopsis liquidambari B3 to produce laccase. This process, which provided the foundation for the subsequent research and for scaled-up laccase production, offers solutions for both rural and urban pollution problems: that is, it reduces the amount of waste material that needs to be disposed of by burning or dumping, and it also produces a valuable product.

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Different laccase detoxification strategies for ethanol production from lignocellulosic biomass by the thermotolerant yeast Kluyveromyces marxianus CECT 10875

Cited by 101 publications

References 33 publications

Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

Laccases as a Potential Tool for the Efficient Conversion of Lignocellulosic Biomass: A Review

Asymmetric bioreduction of β-ketoesters derivatives by Kluyveromyces marxianus: influence of molecular structure on the conversion and enantiomeric excess

Laccase production by Phomopsis liquidambari B3 cultured with food waste and wheat straw as the main nitrogen and carbon sources

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