Different levels of changes induced by the insecticide lindane in cultured C-6 glioma cells

Roux, Françoise; Treich, Irène; Fournier, E

doi:10.1016/0300-483x(80)90103-1

Cited by 4 publications

(1 citation statement)

References 7 publications

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“…Lindane and paraquat concentrations were chosen considering both literature values ( , ) and results previously published (), to study possible toxicological processes while keeping cell viability close to that of controls.…”

Section: Methodsmentioning

confidence: 99%

¹H NMR Investigation of Toxic Effects of Lindane and Paraquat on Hep 3B and Hep G2 Human Hepatoma Cell Lines

Descampiaux¹,

Imbenotte²,

Desenclos³

et al. 1997

Chem. Res. Toxicol.

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Lindane and paraquat induce biochemical changes in the liver. In order to specify their molecular impact at the cellular level, a 300 MHz 1H NMR investigation of hepatoma cell lines Hep 3B and Hep G2 responses was performed. Cells were exposed over 24 h to 50 mg/L lindane (0.178 mM) or to 100 mg/L (0.389 mM) paraquat concentrations. The main observation following exposure to lindane was a decrease in betaine methyl groups (3.26 ppm) which could be related to the steatosis reported by some authors. Specifically, in Hep G2 cells with this pesticide, the glycine peak (3.56 ppm) was lowered, thus confirming that the glycine synthesis pathway involving methionine, choline, and betaine was disturbed by lindane. Moreover, in this hepatoma cell line, the p-chlorobenzoate ion could be detected as a doublet at 7.55 ppm. In Hep 3B cells, paraquat increased betaine and methionine levels, suggesting disturbance in glycine biosynthesis. Possibilities of cellular uptake were considered, and the presence of this herbicide in cells was revealed by spectrophotometric and NMR measurements after chlorhydric hydrolysis, suggesting interaction with cellular components. The impact of paraquat on Hep G2 cells appeared to be located on mitochondrial function, as indicated by the observed decrease in succinate and pyruvate levels.

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Section: Methodsmentioning

confidence: 99%

¹H NMR Investigation of Toxic Effects of Lindane and Paraquat on Hep 3B and Hep G2 Human Hepatoma Cell Lines

Descampiaux¹,

Imbenotte²,

Desenclos³

et al. 1997

Chem. Res. Toxicol.

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Effect of Hexachlorocyclohexane (HCH), Its Isomers, and Metabolites on Hsp70 Expression in TransgenicDrosophila melanogaster

Chowdhuri¹,

Saxena²,

Viswanathan

1999

Pesticide Biochemistry and Physiology

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Rapidity of RNA synthesis in human cells

1985

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Different levels of changes induced by the insecticide lindane in cultured C-6 glioma cells

Cited by 4 publications

References 7 publications

¹H NMR Investigation of Toxic Effects of Lindane and Paraquat on Hep 3B and Hep G2 Human Hepatoma Cell Lines

¹H NMR Investigation of Toxic Effects of Lindane and Paraquat on Hep 3B and Hep G2 Human Hepatoma Cell Lines

Effect of Hexachlorocyclohexane (HCH), Its Isomers, and Metabolites on Hsp70 Expression in TransgenicDrosophila melanogaster

Rapidity of RNA synthesis in human cells

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Different levels of changes induced by the insecticide lindane in cultured C-6 glioma cells

Cited by 4 publications

References 7 publications

1H NMR Investigation of Toxic Effects of Lindane and Paraquat on Hep 3B and Hep G2 Human Hepatoma Cell Lines

1H NMR Investigation of Toxic Effects of Lindane and Paraquat on Hep 3B and Hep G2 Human Hepatoma Cell Lines

Effect of Hexachlorocyclohexane (HCH), Its Isomers, and Metabolites on Hsp70 Expression in TransgenicDrosophila melanogaster

Rapidity of RNA synthesis in human cells

Contact Info

Product

Resources

About

¹H NMR Investigation of Toxic Effects of Lindane and Paraquat on Hep 3B and Hep G2 Human Hepatoma Cell Lines

¹H NMR Investigation of Toxic Effects of Lindane and Paraquat on Hep 3B and Hep G2 Human Hepatoma Cell Lines