Different Secretory Response of Pancreatic Isolated Lobules and Dissociated Acini from Hypothyroid Rats to Exogen TRH

Abstract

This paper analyses the effect of hypothyroidism on pancreatic TRH and somatostatin concentrations, as well as the action of exogen TRH on pancreatic amylase secretion from isolated lobules and dissociated acini of both healthy and hypothyroid rats. In the hypothyroid group, pancreatic TRH and somatostatin increased. In the pancreatic lobules of untreated animals, bethanechol produced stimulatory action that was inhibited by TRH. On the other hand, lobules from hypothyroid rats did not respond to bethanechol stimulation. Acini amylase secretion after bethanechol stimulation was similar in both groups, although hypothyroid animals were more sensitive to the inhibitory effect of TRH. These findings suggest the existence of a factor blocking the amylase secretion in pancreatic lobules. This agent, probably TRH, could be eliminated in the experimental model of dissociated acini.

Hypothyroidism with elevated pancreatic amylase and lipase without clinical symptoms: A case report

“…Hypothyroidism has not been previously reported to lead to elevated pancreatic amylase and lipase without clinical symptoms. A previous study by Blanco-Molina A found that dissociated acini in rats with hypothyroidism showed higher bethanechol-induced amylase secretion than acini in control animals, and the experimental rates were also more sensitive to the inhibitory TRH effect than the controls[ 11 ]. The animals with hypothyroidism were more sensitive to the inhibitory effect of TRH than the control animals.…”