Differential antibody responses in sows and finishing pigs naturally infected with African swine fever virus under field conditions

Luong, H; Lai, Huong Tl.; D., Luc; Ha, Bo X.; Nguyen, Van Giap; Vu, Hiep L.X.

doi:10.1016/j.virusres.2021.198621

Cited by 8 publications

(8 citation statements)

References 26 publications

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“…One study identified antibodies from 23 ASFV-positive pig serum samples, and the results showed that all the samples contained antibodies against p30, p54 and pp62. These results indicate the strong immunogenic properties of p30, p54 and pp62 ( Luong et al, 2022 ).…”

Section: The Major Structural Proteins In Asfv Vaccine Developmentmentioning

confidence: 70%

Advancement in the development of gene/protein-based vaccines against African swine fever virus

Wang,

Huang,

Zhang

et al. 2024

Current Research in Microbial Sciences

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Section: The Major Structural Proteins In Asfv Vaccine Developmentmentioning

confidence: 70%

Advancement in the development of gene/protein-based vaccines against African swine fever virus

Wang,

Huang,

Zhang

et al. 2024

Current Research in Microbial Sciences

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“…As an immunogen, CD2v can elicit adaptive immune responses [ 22 , 36 ]. Indeed, modified vaccinia virus Ankara (MVA) expressing CD2v, P72, and EP153R induced significant cellular immunity characterized by T cell proliferation and IFN-γ production [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

An Intracellular Epitope of ASFV CD2v Protein Elicits Humoral and Cellular Immune Responses

Bai

Zhang

et al. 2023

Animals

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The African swine fever virus (ASFV) causes high mortality in domestic pigs. ASFV encodes an important protein target for subunit vaccine development, CD2v, but its most effective immunological regions are not known. Herein, we generated a monoclonal antibody (mAb) named IF3 by immunizing mice against the intracellular region of the CD2v protein (CD2v-IR). 1F3 specifically recognized CD2v, which is expressed transiently in transfected Sf9 cells and also in inactivated ASFV-infected porcine alveolar macrophage (PAM) cells. The epitope recognized by 1F3 is 264EPSPREP270, which is highly conserved in ASFV genotypes. Immunization of mice with this epitope elicited an increased IgG response, including IgG1 and IgG2a subtypes, and also increased CD8+ T cells and cytokine expression. Overall, these results indicate that this epitope induces both humoral and cellular immune responses that may be used for ASFV-related subunit vaccine design and development.

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“…In contrast to antigen specific ELISAs that have been developed using recombinant ASFV antigens in bacterial [22-24, 26, 27] or insect cells [27,60,61], the assays described here use recombinant proteins produced in mammalian cells, similar to the EP402R/CD2v ELISA developed by Lv et al [25] and the LIPS assay developed by Luong et al to a panel of ASFV antigens (O61R/p12, CP204L/p30, E183L/p54, CP530R/pp62, EP153R/C-type lectin and EP402R/CD2v) [29] to ensure that the recombinant proteins receive mammalian post-translational modifications resembling their native conformation. The ability to utilize unpurified recombinant proteins in cell lysates for the assay simplifies the process [31,32] and enables the development of assays using antigens that are typically difficult to purify, like membrane bound proteins or hard to express proteins with low yields, which is typical of many ASFV proteins that have immunomodulatory properties.…”

Section: Discussionmentioning

confidence: 99%

“…Luciferase based antibody diagnostics has previously been reported for porcine diseases using luciferase immunoprecipitation systems (LIPS) [29,30] and luciferase-linked antibody capture assays (LACAs) [31]. Both LIPS and LACAs detect and quantify antigen specific antibodies indirectly through the capture of antibodies that are bound to recombinant luciferase tagged proteins of interest [31,32].…”

Section: Introductionmentioning

confidence: 99%

Capsid Specific Antibody Responses of Domestic Pigs Immunized with Low Virulent African Swine Fever Virus

Tng¹,

Al-Adwani²,

Pauletto³

et al. 2023

Preprint

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African swine fever (ASF) is a lethal disease in pigs that has grave socio-economic implications worldwide. For the development of vaccines against African swine fever virus (ASFV), immunogenic antigens that generate protective immune responses need to be identified. There are over 150 viral proteins - many of which are uncharacterized – and humoral immunity to ASFV has not been closely examined. To profile antigen specific antibody responses, we developed luciferase-linked antibody capture assays (LACAs) for a panel of ASFV capsid proteins and screened sera from inbred and outbred animals that were previously immunized with low virulent ASFV before challenge with virulent ASFV. Antibodies to B646L/p72, D117L/p17, M1249L and E120R/p14.5 were detected in this study; however, we were unable to detect B438L specific antibodies. Although OURT88/1 induced viremia was observed in the presence of anti-B646L/p72 antibodies as well as B602L antibodies they were associated with recovery from lethal disease in inbred and outbred animals. However, these antibodies did not correlate with protection against Georgia 2007/1 infection. Antibody responses against M1249L and E120R/p14.5 were observed in animals with reduced clinical signs and viremia. Here we present LACAs as a tool for targeted profiling of antigen specific antibody responses to inform vaccine development.

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Differential antibody responses in sows and finishing pigs naturally infected with African swine fever virus under field conditions

Cited by 8 publications

References 26 publications

Advancement in the development of gene/protein-based vaccines against African swine fever virus

Advancement in the development of gene/protein-based vaccines against African swine fever virus

An Intracellular Epitope of ASFV CD2v Protein Elicits Humoral and Cellular Immune Responses

Capsid Specific Antibody Responses of Domestic Pigs Immunized with Low Virulent African Swine Fever Virus

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