Differential Degradation of Early Synthesized Proteins in Dormant and Afterripened Agrostemma githago L. Embryos

Klerk, G.J.M. de

doi:10.1016/s0044-328x(11)80002-4

Cited by 5 publications

(3 citation statements)

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“…When two different protein samples, one labelled with [-^HJleucine and the other with ['*C]leucitie, are tnixed before electrophoresis, differences in banding pattern show up as differences in the distribution of ^H and '*C label over the gel. This result ean be quantified by calculating the difference coefficient (De Klerk, 1982), After electrophoresis, gels were sliced and digested in 0,4 cm^ HjOj, 40%, at 70°C overnight. The samples were taken up in Aqualyte (Baker, Deventer, The Netherlands) and the radioactivities were determined in a liquid seintillation analyzer (Philips PW-4540, Eindhoven, The Netherlands), preset at 100,000 eounts.…”

Section: Extraetion Etectrophoresis and Autoradiographymentioning

confidence: 99%

Qualitative changes in protein synthesis in germinating pollen of Lilium longiflorum after a heat shock

Zhang

Croes

Linskens

1984

Plant Cell & Environment

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Germination and protein synthesis of lily pollen subjeeted to a short heat shock after imbibition are strotigly inhibited. The proteins synthesized after the heat shock were analysed by polyaerylamide gel electrophoresis. The patterns obtained from heat-treated pollen are strikingly difTerent from those of eontrol samples. The difference is nearly completely eliminated by a high concentration of proline in the incubation medium. This proline effect correlates with the protection of pollen germination from high temperature by the amino aeid.

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Section: Extraetion Etectrophoresis and Autoradiographymentioning

confidence: 99%

Qualitative changes in protein synthesis in germinating pollen of Lilium longiflorum after a heat shock

Zhang

Croes

Linskens

1984

Plant Cell & Environment

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“…After being afterripened by 3 months of dry storage at room temperature, the seeds will germinate at both the low and the high temperature (3,23). As a part of our investigations on protein synthesis in ripening D and A A. githago seeds (10)(11)(12)(13)(14), we re-examined the rates of protein synthesis in embryos of D 15 to 30 min behind D embryos, it was unlikely that this should be due to the slower water uptake by A embryos. Since seeds lose water during dry storage (about 14% in 1 year), the low water content of dry A seeds may have damaged the proteinsynthesizing machinery (21).…”

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“…3) The overall differences between the electrophoresis patterns of 3H-and '4C-labeled proteins were evaluated by calculating the DCs. This procedure has been described in detail previously (12). In summary, after electrophoresis each lane was cut into 75 2-mm slices.…”

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Protein Synthesis in Embryos of Dormant and Germinating Agrostemma githago L. seeds

Klerk

Smulders²

1984

Plant Physiol.

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ABSTRACIThe time course of protein synthesis in embryos of dormant and afterripened Agrostemma githago seeds was studied. In embryos of afterripened geminating seeds, protein synthesis increased in three successive stages: (a) concurrent with swelling; (b) during the lag phase between the completion of water uptake and the onset of growth; and (c) immediately after protrusion through the seed coat. Embryos of dormant seeds showed the first increase but not the second unless dormancy was broken by imbibition at 4°C. This indicates that dormancy affects processes prior to the onset of growth. The third increase was largely due to higher oxygen availability after the rupture of the seed coat and not to actual growth. It could also be elicited in dormant embryos by isolating them from the seeds.Electrophoretic analysis of the newly synthesized proteins demonstrated that the patterns of dormant and afterripened embryos became significantly different in both axes and cotyledons only just prior to the onset of axis elongation. Thereafter, the differences became larger.When afterripened or dormant seeds were transferred from a low, germination-permitting to a high, germination-inhibiting temperature, the seeds germinated at the high temperature if they had completed the lag phase to a sufficient extent at the low temperature. This shows that the processes during the lag phase were inhibited by the high temperature while the onset of growth was not affected.The germination process of nondormant seeds can be divided into three successive phases: (a) the uptake of water; (b) a lag period during which processes seem to take place in preparation for growth; and (c) axis elongation together with a rupture of the seed coat (21,28 (9,19,20). In contrast, in light-requiring and in hormone-stimulated seeds the rate ofprotein synthesis exceeds that of untreated, D seeds after the induction but before the onset of growth (16,24,27,30). In all these studies, however, the actual differences in protein synthesis might have been obscured by differential dilution of applied precursors by the en- ' [3H]leucine and 10 mM unlabeled leucine were added. Since the embryos were isolated from the seeds just prior to labeling, this is considered to represent the course of protein synthesis in embryos of intact seeds. In one experiment, imbibition and incubation were carried out at 4°C. The incubation time was then 1 h. In another experiment, various concentrations of unlabeled leucine were added instead of 10 mM.To determine the time course of protein synthesis in isolated embryos, embryos were excised from seeds 6 h after the start of imbibition, preincubated for increasing periods in 10 mM KCI, 1 mm CaC12, and 50 MAg ml-' chloramphenicol after which they were incubated for 30 min in 38 kBq [3H]leucine and 10 mM unlabeled leucine. This will be referred to as the course ofprotein synthesis in isolated embryos.For electrophoretic analysis, samples of 10 embryos were isolated from seeds after various periods of imbibition at 20°C and incubated...

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Fate of Leucine Fed to Dormant and Afterripened Agrostemma githago L. Embryos

Klerk

1982

Zeitschrift für Pflanzenphysiologie

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Differential Degradation of Early Synthesized Proteins in Dormant and Afterripened Agrostemma githago L. Embryos

Cited by 5 publications

References 5 publications

Qualitative changes in protein synthesis in germinating pollen of Lilium longiflorum after a heat shock

Qualitative changes in protein synthesis in germinating pollen of Lilium longiflorum after a heat shock

Protein Synthesis in Embryos of Dormant and Germinating Agrostemma githago L. seeds

Fate of Leucine Fed to Dormant and Afterripened Agrostemma githago L. Embryos

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