Differential distributions of CSE1L/CAS and E-cadherin in the polarized and non-polarized epithelial glands of neoplastic colorectal epithelium

Abstract

Colorectal glands are important functional organs in colorectal tissue and are also the origin of colorectal carcinomas. Epithelial cell polarization of colorectal glands is related to structural integrity and physiological functions of colorectal glands as well as colorectal carcinoma formation. The cellular apoptosis susceptibility (CSE1L/CAS) protein has been shown to induce polarity formation of human colorectal cells in cell culture. E-cadherin expression in epithelial cells is crucial for the establishment and maintenance of epithelial cell polarity. In this study we examined the distributions of CSE1L and E-cadherin in the epithelial glands of normal and neoplastic colorectal epithelium and correlated these to polarity formation in the colorectal glands. Our results showed that CSE1L was differentially stained in the epithelial glands of neoplastic colorectal epithelium, and the staining was related to gland epithelial cell polarization and E-cadherin distribution. CSE1L was associated E-cadherin in GST pull-down experiments and immunoprecipitation assays. Basolateral staining of CSE1L and E-cadherin were seen in the polarized glands of normal and neoplastic colorectal epithelium. Absence of basolateral CSE1L staining in neoplastic epithelium glands was associated with loss of gland epithelial cell polarity, and this was parallel with E-cadherin staining. The non-polarized areas in epithelium glands showed a patchy staining for CSE1L and E-cadherin. These results indicate that examination of CSE1L and E-cadherin distribution in colorectal epithelium glands may be valuable for evaluating the malignance of colorectal disease.

Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage

“…In the negative control, the primary antibody was omitted and replaced by PBS. CRC tissues are known to be positive for CSE1L staining [12]; thus, no positive control was used in this study.…”

“…CSE1L is highly expressed in most cancers, and its expression has been shown to correlate with cancer progression [4-12]. CSE1L is located in both the cytoplasm and the nuclei of cells.…”

Cellular apoptosis susceptibility (CSE1L/CAS) protein in cancer metastasis and chemotherapeutic drug-induced apoptosis

“…The expression of CSE1L was also reported to be higher in the primary and metastatic human colorectal carcinoma compared to the normal colon mucosa ( p < 0.0001) [ 48 ]. Recent study also showed that the distribution CSE1L in the epithelial glands of neoplastic colorectal epithelium was related to the malignance of colorectal cancer [ 49 ].…”

CSE1L, DIDO1 and RBM39 in colorectal adenoma to carcinoma progression

“…P-values were determined using the Chi-Square test levels. Several papers emphasize (vesicular) cytoplasmic staining of CSE1L and propose a role for CSE1L in vesicle translocation and protein secretion [34][35][36]. Proteomics analysis of cell surface and secretomes of CRC cell lines supports the presence of CSE1L protein associated with plasma membranes and secreted microvesicles (supplementary data in de Wit et al [11] and Fijneman et al, [37]).…”