Differential effects of glucose and fructose on hexose metabolism in dog spermatozoa

Rigau, T

doi:10.1530/reprod/123.4.579

Cited by 32 publications

(67 citation statements)

References 10 publications

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“…Facilitated glucose transporters (GLUT) are among the candidates [34,35]. Various glucose transporter isoforms including GLUT1, 2, 3, 5 and 8 have been identified in testicular germ cells and spermatozoa in the rat, bull, dog and man [36][37][38][39][40][41][42]. In the report that the GLUT inhibitor phloretin inhibits osmotic water fluxes in ovine and human spermatozoa, it was suggested that some of these transporters may be involved in water transport [43].…”

Section: Water Transporters Other Than Aqpsmentioning

confidence: 99%

Aquaporins in spermatozoa and testicular germ cells: identification and potential role

Yeung¹

2010

Asian J Androl

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Mammalian spermatozoa have relatively high water permeability and swell readily, as in the hypo-osmotic swelling test used in the andrology clinic. Physiologically, spermatozoa experience changes in the osmolality of the surrounding fluids in both the male and the female tracts on their journey from the testis to the ovum. Sperm volume regulation in response to such osmotic challenges is important to maintain a stable cell size for the normal shape and function of the sperm tail. Alongside ion channels for the fluxes of osmolytes, water channels would be crucial for sperm volume regulation. In contrast to the deep knowledge and numerous studies on somatic cell aquaporins (AQPs), the understanding of sperm AQPs is limited. Among the 13 AQPs, convincing evidence for their presence in spermatozoa has been confined to AQP7, AQP8 and AQP11. Overall, current findings indicate a major role of AQP8 in water influx and efflux for sperm volume regulation, which is required for natural fertilization. The preliminary data suggestive of a role for AQP7 in sperm glycerol metabolism needs further substantiation. The association of AQP11 with the residual cytoplasm of elongated spermatids and the distal tail of spermatozoa supports the hypothesis of more than just a role in conferring water permeability and also in the turnover and recycling of surplus cellular components made redundant during spermiogenesis and spermiation. This would be crucial for the maintenance of a germinal epithelium functioning efficiently in the production of spermatozoa.

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Section: Water Transporters Other Than Aqpsmentioning

confidence: 99%

Aquaporins in spermatozoa and testicular germ cells: identification and potential role

Yeung¹

2010

Asian J Androl

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“…The first point is the fact that the optimal utilization of sugars by sperm is reached to determined concentrations of this sugars. For instance, the optimal utilization rate of glucose by sperm from dog and boar is reached to very low concentrations of the sugar, at about 0.1 mM [16,36,37,45]. This indicates that the addition of low concentrations of sugar to the extenders would be enough to maintain sperm energy levels.…”

Section: Modulation Of Energy Metabolism As a Tool To Improve Ia Resultsmentioning

confidence: 99%

“…In fact, both fructose and sorbitol are typical vegetal sugars, without any significant presence in animals, excepting in mammalian seminal plasma. It is noteworthy that the utilization of sugars like fructose, sorbitol and mannose by sperm of species like boar and dog of is in fact less effective in order to obtain energy than that of glucose [36,45]. The greater effectiveness of glucose is mainly linked to a greater sensitivity to the hexokinase system, which phosphorylates sugars as a first step in their metabolization pathway [36,45].…”

Section: Energy Sources For Mammalian Spermatozoamentioning

confidence: 99%

“…It is noteworthy that the utilization of sugars like fructose, sorbitol and mannose by sperm of species like boar and dog of is in fact less effective in order to obtain energy than that of glucose [36,45]. The greater effectiveness of glucose is mainly linked to a greater sensitivity to the hexokinase system, which phosphorylates sugars as a first step in their metabolization pathway [36,45]. Taking into account this lower efficiency, it is difficult to understand the biological logics to utilize sugars like fructose or sorbitol as mere energy sources for sperm.…”

Section: Energy Sources For Mammalian Spermatozoamentioning

confidence: 99%

“…However, it is difficult to understand why seminal plasma in all species does not contain glucose as their main energy source, since glucose is the most important energy-producing monosaccharide for all of mammalian tissues. Instead of this, seminal plasma contains other sugars, specially fructose, but also sorbitol and other [6,28,32,33,36,42,45], which are not as efficient as glucose as primary energy sources (see as an example in boar sperm [36]. Again, investigators can only speculate on this point.…”

Section: Introductionmentioning

confidence: 99%

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Energy Management of Mature Mammalian Spermatozoa

Rodríguez‐Gil¹

2013

Success in Artificial Insemination - Quality of Semen and Diagnostics Employed

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Specific Order in the Appearance of Protein Tyrosine Phosphorylation Patterns Is Functionally Coordinated With Dog Sperm Hyperactivation and Capacitation

Petrunkina¹,

Simon²,

Günzel‐Apel³

et al. 2003

Journal of Andrology

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The aims of the present study were to characterize a slow capacitation system that records initial changes in the sperm membrane state, and, using a canine model, to order the specific protein tyrosine phosphorylation signaling in the sequence of capacitational events and to associate them with hyperactivated motility. Dog sperm washed through Percoll were incubated in complete bicarbonate Tyrode medium for 6 hours in 5% CO(2). Capacitation was evaluated using chlortetracycline staining. Tyrosine phosphorylation patterns were assessed by immunocytochemistry. Parallel to this, a computer-assisted motility analysis was performed. Significant changes in the percentage of capacitated and acrosome-reacted cells were first observed after 90 minutes, increasing in a linear manner during further incubation (P <.05). Changes in the percentage of capacitated cells were accompanied by motility changes. During incubation, a strictly sequential phosphorylation of sperm tail (midpiece, principal piece, and end piece) and head proteins was observed. According to an analysis of kinetics, phosphorylation of head proteins occurred after the tail became completely phosphorylated. Changes in head phosphorylation progressed at the same rates as capacitation and acrosome reaction. Sperm motility, curvilinear velocity, average path velocity, straight line velocity, and lateral head displacement were correlated positively or negatively with phosphorylation of midpiece or end piece proteins, respectively. The bicarbonate-stimulated increases in cyclic adenosine monophosphate levels and changes in protein phosphatase activity may be involved in the signaling system that controls membrane changes and motility in dog sperm. Phosphorylation kinetics of sperm proteins are potentially useful for diagnostic purposes to characterize the response of individual males to fertilizing conditions.

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Differential effects of glucose and fructose on hexose metabolism in dog spermatozoa

Cited by 32 publications

References 10 publications

Aquaporins in spermatozoa and testicular germ cells: identification and potential role

Aquaporins in spermatozoa and testicular germ cells: identification and potential role

Energy Management of Mature Mammalian Spermatozoa

Specific Order in the Appearance of Protein Tyrosine Phosphorylation Patterns Is Functionally Coordinated With Dog Sperm Hyperactivation and Capacitation

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