Differential effects of the steaming time and frequency for manufactured red Liriope platyphylla on nerve growth factor secretion ability, nerve growth factor receptor signaling pathway and regulation of calcium concentration

Abstract

The herb Liriope platyphylla (LP) has been considered to have curative properties for diabetes, asthma and neurodegenerative disorders. To examine the effects of steaming time and frequency of manufactured red LP (RLP) on the nerve growth factor (NGF) secretion ability and NGF receptor signaling pathway, the NGF concentration, cell differentiation, NGF signaling pathway and calcium concentration were analyzed in neuronal cells treated with several types of LPs manufactured under different conditions. The maximum NGF secretion was observed in B35 cells treated with 50 µg/ml LP extract steamed for 9 h (9-SLP) and with two repeated steps (3 h steaming and 24 h air-dried) carried out 7 times (7-SALP). No significant changes in viability were detected in any of the cells treated with the various LPs, with the exception of 0-SLP and 0-SALP. In addition, PC12 cell differentiation was induced by treatment with the NGF-containing conditional medium (CM) collected from the RLP-treated cells. The levels of TrkA and extracellular signal-regulated kinase (ERK) phosphorylation in the high affinity NGF receptor signaling pathway were significantly higher in the cells treated with 3-SLP or 1-SALP/3-SALP CM compared with those treated with the vehicle CM. In the low affinity NGF receptor pathway, the expression levels of most components were higher in the 9-, 15- and 24-SALP CM-treated cells compared with the vehicle CM-treated cells. However, this level was significantly altered in cells treated with 3-SALP CM. Furthermore, an examination of the RLP function on calcium regulation revealed that only the LP- or RLP-treated cells exhibited changes in intracellular and extracellular calcium levels. RLP induced a significant decrease in the intracellular calcium levels and an increase in the extracellular calcium levels. These results suggest the possibility that steaming-processed LP may aid in the relief of neurodegenerative diseases through the NGF secretion ability and NGF signaling pathway.

Neuroprotective effects of Liriope platyphylla extract against hydrogen peroxide-induced cytotoxicity in human neuroblastoma SH-SY5Y cells

“…Previous reports evaluated the relationship between the active compounds in LP and neuronal differentiation/brain-derived neurotrophic factor (BDNF)-mediated memory. Manufactured red LP increased NGF secretion and ERK phosphorylation in the B35 neuronal cell line and in PC12 cells [ 26 ]. Previous studies demonstrated that LP contains several active compounds, including spicatoside A, ophiopogonin A – D, and methylophiopogonanone A, B. Spicatoside A exerts its neurogenic effects by inducing neurite outgrowth and activating ERK and the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway in PC12 cells [ 27 ].…”

Aqueous extracts of Liriope platyphylla induced significant laxative effects on loperamide-induced constipation of SD rats

“…L. platyphylla is also known to potently inhibit airway inflammation and hyperresponsiveness in a murine model of asthma by modulating the relationship between Th1/Th2 cytokine imbalance [13] and atopic dermatitis induced by phthalic anhydride treatment [14,15]. Significant alteration of nerve growth factor (NGF) secretion and its related signaling pathway involving NGF receptors TrkA and p75 NTR has been observed in extracts and compounds isolated from L. platyphylla -treated PC12 cells [16,17]. L. platyphylla has also demonstrated potential for the treatment of obesity and diabetes [18,19].…”

Phytochemical and pharmacological overview on <i>Liriopes radix</i>

“…The culture supernatant from B35 and C6 cells with LR extract for 24 h were treated into PC12 cells and the differentiation level were significantly increased [49]. Moreover, B35 cells conditioned medium treated to PC12 cells showed significantly higher the levels of tropomyosin receptor kinase A (TrkA), ERK phosphorylation, and extracellular calcium levels with a significant decrease in the intracellular calcium levels [50]. LR extracts induced NGFsecretion and NGF mRNA expression with high cell viability in vitro and significantly increased the NGF mRNA and hippocampus TrkA and inhibited the p75 NTR signaling pathways in C57BL/6 mice [51].…”