Differential expression of adipose tissue proteins between obesity‐susceptible and ‐resistant rats fed a high‐fat diet

Joo, Jeong In; Oh, Tae Seok; Kim, Dong Hyun; Choi, Duk Kwon; Wang, Xia; Choi, Jung-Won; Yun, Jong Won

doi:10.1002/pmic.201000515

Cited by 41 publications

(19 citation statements)

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“…2), protein spots were identified by MALDI-TOF-MS (Table 3), and database searches were performed with high confidence based on high score and sequence coverage. For the results, a total of 1,045 individual matched spots ranging in mass from 6 to 240 kDa between pH 3-10 were detected, and obtained WAT protein maps represented patterns that were similar to our previous results [26,27,28,34]. Fifty-nine spots were identified with statistically different intensities between the ND and OP groups.…”

Section: Resultssupporting

confidence: 80%

“…2-DE experiments were conducted using the previous methods outlined by our laboratory [26,27,34]. Briefly, immobilized pH gradient (IPG)-isoelectric focusing (IEF) of WAT samples was performed on pH 3-10 and 18 cm IPG DryStrips (GE Healthcare, Buckinghamshire, UK) in a PROTEIN IEF cell (Bio-Rad) using the protocol recommended by the manufacturer.…”

Section: Methodsmentioning

confidence: 99%

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Differential Protein Expression in White Adipose Tissue from Obesity-Prone and Obesity-Resistant Mice in Response to High Fat Diet and Anti-Obesity Herbal Medicines

Kim

Park²,

Choi³

et al. 2015

Cell Physiol Biochem

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Background: One of the most interesting issues in obesity research is why certain humans are obesity-prone (OP) while others are obesity-resistant (OR) upon exposure to a high-calorie diet. However, the pathways responsible for these phenotypic differences are still largely unknown. Methods: In order to discover marker molecules determining susceptibility and/or resistance to obesity in response to high fat diet (HFD) or anti-obesity herbal medicine (TH), we conducted comparative proteomic analysis of white adipose tissue (WAT) from OP, OR, as well as TH-treated mice. Results: OP mice fed HFD gained approximately 33% more body weight than OR mice, and TH significantly reduced body weight gain in HFD-fed mice by 30%. These mice were further subjected to proteomic analysis using two-dimensional electrophoresis (2-DE) combined with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Proteomic data revealed 59 spots that were differentially regulated from a total of 1,045 matched spots, and 57 spots of these were identified as altered WAT proteins between OP and OR mice by peptide mass finger printing. Interestingly, 45 proteins were similarly regulated in OR mice in response to TH treatment. Of these, 10 proteins have already been recognized in the context of obesity; however, other proteins involved in obesity susceptibility or resistance were identified for the first time in the present study. Conclusion: Our results suggest that TH actively contributed to body weight reduction in HFD-fed obese mice by altering protein regulation in WAT, and it was also found that TH-responsive proteins can be used as potent molecules for obesity treatment.

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Section: Resultssupporting

confidence: 80%

Section: Methodsmentioning

confidence: 99%

Differential Protein Expression in White Adipose Tissue from Obesity-Prone and Obesity-Resistant Mice in Response to High Fat Diet and Anti-Obesity Herbal Medicines

Kim

Park²,

Choi³

et al. 2015

Cell Physiol Biochem

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“…These results suggest that DJ-1 deficiency induces the reduction in insulin secretion as previously reported17. On the other hand, several previous reports showed that DJ-1 expression was higher in obesity-resistant rats and suggested a possible role of DJ-1 in adipogenesis associated with androgen receptor-mediated signaling pathway313233. It is supposed that androgen inhibits adipogenesis of mouse pluripotent cells.…”

Section: Discussionsupporting

confidence: 83%

DJ-1 contributes to adipogenesis and obesity-induced inflammation

Kim

Jang

Choi

et al. 2014

Sci Rep

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Adipose tissue functions as an endocrine organ, and the development of systemic inflammation in adipose tissue is closely associated with metabolic diseases, such as obesity and insulin resistance. Accordingly, the fine regulation of the inflammatory response caused by obesity has therapeutic potential for the treatment of metabolic syndrome. In this study, we analyzed the role of DJ-1 (PARK7) in adipogenesis and inflammation related to obesity in vitro and in vivo. Many intracellular functions of DJ-1, including oxidative stress regulation, are known. However, the possibility of DJ-1 involvement in metabolic disease is largely unknown. Our results suggest that DJ-1 deficiency results in reduced adipogenesis and the down-regulation of pro-inflammatory cytokines in vitro. Furthermore, DJ-1-deficient mice show a low-level inflammatory response in the high-fat diet-induced obesity model. These results indicate previously unknown functions of DJ-1 in metabolism and therefore suggest that precise regulation of DJ-1 in adipose tissue might have a therapeutic advantage for metabolic disease treatment.

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“…Previously, we observed increased expression of CAV1 in adipose tissues of obesity- resistant SD male rats fed a HFD [43]. In this sense, we can hypothesize that diet affects the expression of CAV1 in a sex and site-specific manner.…”

Section: Introductionmentioning

confidence: 71%

“…Based on our previous finding that CAV1 is highly expressed in adipose tissues in obesity-resistant rats fed a HFD [43], we wished to determine exactly which factors are involved in higher CAV1 expression for obesity resistance in HFD-fed rats as well as what different physiological programs exist between male and female rats. In this sense, we hypothesized that CAV1 may act as a potent molecular target for sex hormone-dependent regulation of adipocyte metabolism.…”

Section: Discussionmentioning

confidence: 99%

Sex-Dependent Expression of Caveolin 1 in Response to Sex Steroid Hormones Is Closely Associated with Development of Obesity in Rats

et al. 2014

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Caveolin-1 (CAV1) is a conserved group of structural membrane proteins that form special cholesterol and sphingolipid-rich compartments, especially in adipocytes. Recently, it has been reported that CAV1 is an important target protein in sex hormone-dependent regulation of various metabolic pathways, particularly in cancer and diabetes. To clarify distinct roles of CAV1 in sex-dependent obesity development, we investigated the effects of high fat diet (HFD) and sex steroid hormones on CAV1 expression in adipose tissues of male and female rats. Results of animal experiments revealed that estrogen (17-β-estradiol, E2) and androgen (dihydrotestosterone, DHT) had opposite effects on body weight gain as well as on the regulation of CAV1, hormone sensitive lipase (HSL) and uncoupling protein 1 (UCP1) in adipose tissues. Furthermore, sex hormone receptors and aromatase were differentially expressed in a sex-dependent manner in response to E2 and DHT treatments. In vivo data were confirmed using 3T3-L1 and HIB1B cell lines, where Cav1 knock down stimulated lipogenesis but suppressed sex hormone receptor signaling proteins. Most importantly, co-immunoprecipitation enabled the identification of previously unrecognized CAV1-interacting mitochondrial or lipid oxidative pathway proteins in adipose tissues. Taken together, current data showed that CAV1 may play important preventive role in the development of obesity, with more prominent effects in females, and proved to be an important target protein for the hormonal regulation of adipose tissue metabolism by manipulating sex hormone receptors and mitochondrial oxidative pathways. Therefore, we can report, for the first time, the molecular mechanism underlying the effects of sex steroid hormones in the sex-dimorphic regulation of CAV1.

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Differential expression of adipose tissue proteins between obesity‐susceptible and ‐resistant rats fed a high‐fat diet

Cited by 41 publications

References 100 publications

Differential Protein Expression in White Adipose Tissue from Obesity-Prone and Obesity-Resistant Mice in Response to High Fat Diet and Anti-Obesity Herbal Medicines

Differential Protein Expression in White Adipose Tissue from Obesity-Prone and Obesity-Resistant Mice in Response to High Fat Diet and Anti-Obesity Herbal Medicines

DJ-1 contributes to adipogenesis and obesity-induced inflammation

Sex-Dependent Expression of Caveolin 1 in Response to Sex Steroid Hormones Is Closely Associated with Development of Obesity in Rats

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