Differential expression of heat-shock proteins in F2 offspring from F1 hybrids produced between thermally selected and normal rainbow trout strains

Ojima, Nobuhiko; Mekuchi, Miyuki; Ineno, Toshinao; Tamaki, Koichi; Kera, Akio; Kinoshita, Shigeharu; Asakawa, Shuichi; Watabe, Shugo

doi:10.1007/s12562-012-0523-3

Cited by 11 publications

(7 citation statements)

References 25 publications

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“…The Australian hatchery fish were genetically more homozygous than their North American counterparts. Furthermore, a study of gene expression in the rainbow trout introduced to Japan (Ojima et al, 2012;Tan et al, 2012) revealed a higher expression of heat shock genes after the strain was raised at high temperatures (20-24°C, occasionally 30°C) in summer for more than 14 successive generations. Similarly, transplanted sticklebacks (Gasterosteus aculeatus) became more cold tolerant in just three generations (Barrett et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Selection for upper thermal tolerance in rainbow trout (Oncorhynchus mykissWalbaum)

Chen

Snow

Lawrence

et al. 2015

Journal of Experimental Biology

121

122

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Rainbow trout (Oncorhynchus mykiss Walbaum) in southern Western Australia have undergone passive selection for over 19 generations to survive high water temperatures. Based on the conceptual model of 'oxygen-and capacity-limited thermal tolerance', we measured critical thermal maximum (CT max ), maximum heart rate ( f H,max ) and aerobic scope to test the hypothesis that these rainbow trout can maintain aerobic scope at high temperatures through a robust cardiac performance supporting oxygen delivery. Across five family groups CT max averaged 29.0±0.02°C. Aerobic scope was maximized at 15.8±0.3°C (T opt ), while the upper pejus temperature (T pej , set at 90% of maximum aerobic scope) was 19.9±0.3°C. Although aerobic scope decreased at temperatures above T opt , the value at 25°C remained well over 40% of the maximum. Furthermore, pharmacologically stimulated f H,max increased with temperature, reaching a peak value between 23.5±0.4 and 24.0±0.4°C (T max ) for three family groups. The Arrhenius breakpoint temperature (T AB ) for f H,max was 20.3±0.3 to 20.7±0.4°C, while the average Q 10 breakpoint temperature (T QB , when the incremental Q 10 <1.6) for f H,max was 21.6±0.2 to 22.0±0.4°C. Collectively, f H,max progressively became less temperature dependent beyond 20°C (T AB and T QB ), which coincides with the upper T pej for aerobic scope. Although upper thermal performance indices for both aerobic scope and f H,max were compared among family groups in this population, appreciable differences were not evident. Compared with other populations of rainbow trout, the present assessment is consistent with the prediction that this strain has undergone selection and shows the ability to tolerate higher water temperatures.

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Section: Discussionmentioning

confidence: 99%

Selection for upper thermal tolerance in rainbow trout (Oncorhynchus mykissWalbaum)

Chen

Snow

Lawrence

et al. 2015

Journal of Experimental Biology

121

122

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“…Two generations of yamame were selected based on their tolerance to high temperature before our experiment. Then, these yamame were tested by exposure to 27.5 °C and were selected based on the TLE [20]. The fish showing more than 80 min of TLE were selected as having high-temperature resistance (F0); we then produced F1 by mating F0.…”

Section: Methodsmentioning

confidence: 99%

“…Many studies have investigated the differentially expressed genes (DEGs) during the response to heat stress in fish; for instance, gene expression of Chinese minnow from the northern region showed greater thermal tolerance than the southern species [18], and the specific regulation patterns indicated a difference between imported rainbow trout and a local breeding strain [16,19]. The thermal-tolerant rainbow trout showed higher expression levels of HSPs than the low thermal-tolerant group under heat stress in fin and gill tissues [9,20]. In addition, HSPs were also highly expressed in other tissues, such as the brain, liver, muscle, and heart, in the thermally selected strains of rainbow trout [13].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis of Yamame (Oncorhynchus masou) in Normal Conditions after Heat Stress

Kraitavin

Yoshitake

Igarashi

et al. 2019

Biology

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Understanding the mechanism of high-temperature tolerance in cold-freshwater fish is crucial for predicting how certain species will cope with global warming. In this study, we investigated temperature tolerance in masu salmon (Oncorhynchus masou, known in Japan as ‘yamame’), an important aquaculture species. By selective breeding, we developed a group of yamame (F2) with high-temperature tolerance. This group was subjected to a high-temperature tolerance test and divided into two groups: High-temperature tolerant (HT) and non-high-temperature tolerant (NT). RNA was extracted from the gill and adipose fin tissues of each group, and the mRNA expression profiles were analyzed using RNA sequencing. A total of 2893 differentially expressed genes (DEGs) from the gill and 836 from the adipose fin were identified by comparing the HT and NT groups. Functional analyses were then performed to identify associated gene ontology (GO) terms and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The HT group showed a high expression of heat shock protein 70 (HSP70) gene and enriched gene expression in the extracellular matrix (ECM), cell junction, and adhesion pathways in gill tissues compared to the NT group. The HT group also exhibited highly expressed genes in glycolysis and showed lower expression of the genes in the p53 signaling pathway in adipose fin tissues. Taken together, the difference of expression of some genes in the normal condition may be responsible for the difference in heat tolerance between the HT and NT yamame in the heat stress condition.

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“…Therefore, most quantitative analyses of fish proteins by Western blot use antibodies made against a different species (or even a mammalian or other more distantly evolutionarily related homolog) that are not suitable to distinguish individual isoforms (e.g. 3,26,27). The present study also investigates whether salinity-induced changes in ionocyte number and size are reflected in abundances of mitochondrial proteins, whether there is disparity in how different mitochondrial proteins are regulated in response to salinity stress, and which mitochondrial proteins are most affected by salinity stress.…”

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confidence: 99%

Quantitative Molecular Phenotyping of Gill Remodeling in a Cichlid Fish Responding to Salinity Stress

Kültz

Gardell

et al. 2013

Molecular & Cellular Proteomics

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A two-tiered label-free quantitative (LFQ) proteomics workflow was used to elucidate how salinity affects the molecular phenotype, i.e. proteome, of gills from a cichlid fish, the euryhaline tilapia (Oreochromis mossambicus). The workflow consists of initial global profiling of relative tryptic peptide abundances in treated versus control samples followed by targeted identification (by MS/MS) and quantitation (by chromatographic peak area integration) of validated peptides for each protein of interest. Fresh water acclimated tilapia were independently exposed in separate experiments to acute short-term (34 ppt) and gradual long-term (70 ppt, 90 ppt) salinity stress followed by molecular phenotyping of the gill proteome. The severity of salinity stress can be deduced with high technical reproducibility from the initial global label-free quantitative profiling step alone at both peptide and protein levels. However, an accurate regulation ratio can only be determined by targeted label-free quantitative profiling because not all peptides used for protein identification are also valid for quantitation. Of the three salinity challenges, gradual acclimation to 90 ppt has the most pronounced effect on gill molecular phenotype. Known salinity effects on tilapia gills, including an increase in the size and number of mitochondria-rich ionocytes, activities of specific ion transporters, and induction of specific molecular chaperones are reflected in the regulation of abundances of the corresponding proteins. Moreover, specific protein isoforms that are responsive to environmental salinity change are resolved and it is revealed that salinity effects on the mitochondrial proteome are nonuniform. Furthermore, protein NDRG1 has been identified as a novel key component of molecular phenotype restructuring during salinity-induced gill remodeling. In conclusion, besides confirming known effects of salinity on gills of euryhaline fish, molecular phenotyping reveals novel insight into proteome changes that underlie the remodeling of tilapia gill epithelium in response to environmental salinity change. Molecular & Cellular Proteomics 12: 10.1074/ mcp.M113.029827, 3962-3975, 2013.Euryhaline fish are capable of living in fresh water (FW), 1 brackish water (BW), seawater (SW), and hypersaline water (ϾSW). They adjust transepithelial ion transport across gill epithelium when challenged by an environmental salinity change (1). Acclimation from hyposmotic (relative to plasma, e.g. FW) to hyperosmotic (relative to plasma, e.g. SW) environments is accompanied by extensive remodeling of gill epithelium, the most prominent feature of which is an increase in the number and size of salt-secretory, mitochondria-rich ionocytes (2). In addition, molecular chaperones and distinct sets of transport proteins are activated when euryhaline fish are challenged by increasing environmental salinity (3)(4)(5). A euryhaline fish species in which these physiological responses have been observed is the Mozambique tilapia, Oreochromis mossambicus (6 -8). Tilapi...

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Differential expression of heat-shock proteins in F2 offspring from F1 hybrids produced between thermally selected and normal rainbow trout strains

Cited by 11 publications

References 25 publications

Selection for upper thermal tolerance in rainbow trout (Oncorhynchus mykissWalbaum)

Selection for upper thermal tolerance in rainbow trout (Oncorhynchus mykissWalbaum)

Transcriptome Analysis of Yamame (Oncorhynchus masou) in Normal Conditions after Heat Stress

Quantitative Molecular Phenotyping of Gill Remodeling in a Cichlid Fish Responding to Salinity Stress

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