Differential expression of long non‐coding RNAs are related to proliferation and histological diversity in follicular lymphomas

Abstract

Long non-coding RNAs (lncRNAs) comprise a family of non-coding transcripts that are emerging as relevant gene expression regulators of different processes, including tumour development. To determine the possible contribution of lncRNA to the pathogenesis of follicular lymphoma (FL) we performed RNA-sequencing at high depth sequencing in primary FL samples ranging from grade 1-3A to aggressive grade 3B variants using unpurified (n = 16) and purified (n = 12) tumour cell suspensions from nodal samples. FL grade 3B had a significantly higher number of differentially expressed lncRNAs (dif-lncRNAs) with potential target coding genes related to cell cycle regulation. Nine out of the 18 selected dif-lncRNAs were validated by quantitative real time polymerase chain reaction in an independent series (n = 43) of FL. RP4-694A7.2 was identified as the top deregulated lncRNA potentially involved in cell proliferation. RP4-694A7.2 silencing in the WSU-FSCCL FL cell line reduced cell proliferation due to a block in the G1/S phase. The relationship between RP4-694A7.2 and proliferation was confirmed in primary samples as its expression levels positively related to the Ki-67 proliferation index. In summary, lncRNAs are differentially expressed across the clinico-biological spectrum of FL and a subset of them, related to cell cycle, may participate in cell proliferation regulation in these tumours.

Long non-coding RNA RP4-694A7.2 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis through the Regulation of PSAT1

“…These results showed that RP4-694A7.2 is an oncogene that promotes HCC development and metastasis. Our results that RP4-694A7.2 is related to increased tumor cell proliferation was consistent with those of a previously published study 19 .…”

“…We found that RP4-694A7.2 expression is higher in HCC malignant tissues than in normal liver tissues. Recently, RP4-694A7.2 has been found in some solid cancers 14 , 19 ; however, its expression patterns and functions in HCC are still unknown. Our results suggested that RP4-694A7.2 is upregulated in HCC and might contribute to tumorigenesis.…”

“…RP4-694A7.2 has been proven to play an oncogenic role in the cancer development and pathogenesis; it may participate in cell-cycle control, and its silencing reduced the S-phase fraction together with a block of the G1/S phase 19 . Our results further showed that numerous proteins that interact with RP4-694A7.2 are associated with catalytic activity and molecular function regulation.…”

RETRACTED ARTICLE: Long noncoding RNA HAGLROS promotes the process of mantle cell lymphoma by regulating miR-100/ATG5 axis and involving in PI3K/AKT/mTOR signal

“…Long noncoding RNAs (lncRNAs), transcripts with longer than 200 nucleotides in length, have emerged as important regulators of disease processes including cancer [5]. Several lncRNAs have been shown to exhibit a potential role in lymphomagenesis [6,7]. Usually, lncRNAs will run their functions through complete regulatory correlations, such as by targeting genes, proteins, or miRNAs [5].…”

Super-Enhancer LncRNA LINC00162 Promotes Progression of Bladder Cancer

“…In recent years, increasing evidence has suggested that lncRNAs can participate in the regulation of various levels of chromosome modification, RNA transcription, and protein translation in physiological cell processes (Hon et al, 2017;Park et al, 2018;Podbevsek et al, 2018). In addition, lncRNAs can also have important effects on various stages of cell physiology (Misawa et al, 2016;Roisman et al, 2019;Xu et al, 2019). At the present time, a large amount of literature has shown that lncRNAs can regulate the development of bladder cancer in various ways.…”