Differential expression of mRNA for Th1 and Th2 cytokine-associated transcription factors and suppressors of cytokine signalling in peripheral blood mononuclear cells of patients with atopic dermatitis

Abstract

SUMMARYAtopic dermatitis is characterized by Th2-dominant immunity. Recently many intracellular molecules have been reported to regulate cytokine expression and T cell differentiation. GATA-3 and T-box expressed in T cells (T-bet) are transcription factors that play a critical role in the development of Th2 and Th1 immunity, respectively. Suppressor of cytokine signalling (SOCS)-3 and SOCS-5, are negative regulators of the cytokine signalling induced by IL-12 and IL-4, respectively. Txk is a transcription factor that activates IFN-g gene directly. The present study was designed to identify intracellular molecules that are responsible for the pathogenesis and the imbalance of cytokines in atopic dermatitis. Semiquantitative RT-PCR revealed that in peripheral blood mononuclear cells without any stimulation the levels of mRNA for GATA-3 and SOCS-3 were elevated, the levels of mRNA for Txk were depressed and the levels of mRNA for T-bet and SOCS-5 were comparable in patients with atopic dermatitis as compared with healthy controls. In addition, successful therapy normalized levels of mRNA for GATA-3 and Txk, although those for the others including IL-4, IL-5, IL-10, IL-13 and IFN-g did not change. Levels of Txk mRNA correlated with those of IFN-g , while the mRNA levels of the other regulators did not correlate with those of any of the cytokines. These results suggest GATA-3 and Txk might be involved in skin lesions, while SOCS-3 might be associated with an imbalance of cytokines that is difficult to normalize in atopic dermatitis.

Cytokines Levels in Children Affected by Atopic and Nonatopic Eczema

“…Data from skin biopsies obtained from uninvolved skin of eczematous patients indicate that acute skin lesions contain significantly more cells producing IL-4, IL-5 and IL-13, but not IFN compared with chronic lesions [5]. In chronic lesions cells expressing IL-4 and IL-13 were significantly decreased, while cells expressing IL-5 and IFN were increased.…”

Eosinophilia triggers changes in IL-5, eotaxin and IL-17, and acts as a prognostic biomarker for atopic dermatitis

“…AD is characterized by sino-pulmonary infections, dermatitis, cutaneous viral infections, altered eosinophil levels, elevated serum IgE, squamous cell carcinomas, and a high incidence of food allergies [12][13][14]. Aarkawa et al reported that different cytokines are present in the peripheral blood mononuclear cells of patients with atopic dermatitis [15]. Therefore, in this study, we investigated the prognostic value of eosinophil and related cytokines in atopic dermatitis.…”

Gata-3 Expression in Peripheral Blood Lymphocytes of Patients With Bronchial Asthma

“…Больные БА характеризуются повышением экспрессии STAT6 и активности IL-4 сигнализации. Выявленное нами повыше-ние экспрессии транскрипционного фактора GATA-3 при бронхиальной астме согласуется с данными литературы [5,9] о повышении его экспрессии при атопии и представляется вполне закономерным, поскольку, по данным некото-рых авторов [25,26], STAT6 может увеличивать экспрессию GATA-3. В этой связи представляют интерес выявленные нами положительные кор-реляционные связи между уровнем экспрессии STAT6 и GATA-3.…”

“…В частности, при исследовании мононуклеров периферической крови больных атопическим дерматитом [5] в отсутствие какой-либо стиму-ляции определялось существенное повышение уровней экспрессии GATA-3 и SOCS3 (супрес-сора цитокиновой сигнализации) в сравнении с практически здоровыми лицами.…”