Differential Expression of Proteins during Healing of Cutaneous Wounds in Experimental Normal and Chronic Models

Shukla, Arti; Dubey, Megha; Srivastava, Ranjana; Srivastava, Bhavana

doi:10.1006/bbrc.1998.8286

Cited by 75 publications

(69 citation statements)

References 24 publications

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“…rAAV particles were then purified by CsCl gradient centrifugation in a SW41Ti rotor at 288,000×g for 36 h. Twelve to sixteen fractions of ten drops each were collected by inserting a G-21 needle below the rAAV band and their refractive index was determined. The six fractions with index closest to 1.3715 (corresponding to a density of 1.40 g/cm 3 ) were dialiyzed against phosphate buffered saline (PBS) at 4°C overnight and stored at −80°C. rAAV titres were determined by measuring the copy number of viral genomes in pooled, dialyzed gradient fractions.…”

Section: Methodsmentioning

confidence: 99%

“…The formation of new blood vessels provides a route for oxygen and nutrient delivery, as well as a conduit for components of the inflammatory response. Healing is concomitant with an increasing release of angiogenic growth factors from macrophages and keratinocytes and its impairment leads to a delay in skin repair [3,4,5,6,7]. Vascular endothelial growth factor (VEGF) is one of the most potent angiogenic cytokines which promotes all steps in the angiogenic cascade [8].…”

Section: Introductionmentioning

confidence: 99%

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Adeno-associated viral vector-mediated human vascular endothelial growth factor gene transfer stimulates angiogenesis and wound healing in the genetically diabetic mouse

et al. 2003

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Aims/hypothesis. We studied the gene therapy efficacy of diabetes-associated wound healing disorder with an adeno-associated virus (AAV) vector expressing the 165-amino acid isoform of human vascular endothelial growth factor-A (VEGF-A) by using an incisional skin-wound model produced on the back of female diabetic C57BL/KsJ db+/db+ mice and their normal littermates (db+/+m). Methods. Animals were randomized to receive intradermally into the wound edges either rAAV-LacZ (a control gene), or rAAV-VEGF165. Animals were killed on different days (7 and 14 days after skin injury) and wounded skin tissues were used for gene marker studies, histological evaluation and immunohistochemistry, and wound breaking strength analysis. Furthermore we studied the VEGF mature protein in the wounds. Results. We found that AAV vectors are highly efficient for gene transfer to the mouse skin, displaying an exquisite tropism for the panniculus carnosus by using the beta-galactosidase activity assay. We confirmed the increased expression of the angiogenic factor at day 7 by measuring the wound content of the mature protein. Delivery of VEGF165 to incisional skin wounds of diabetic mice resulted in a remarkable induction of new vessel formation with consequent improvement in the wound healing process. The rAAV-VEGF165 gene improved wound healing in diabetic mice through the stimulation of angiogenesis, reepithelization, synthesis and maturation of extracellular matrix. Moreover the recombinant AAV encoding the human VEGF165 increased the breaking strenght of the wound and enhanced the wound content of VEGF. Conclusion/interpretation. Our study suggests that VEGF gene transfer might represent a new approach to treat wound healing disorders associated with diabetes. [Diabetologia (2003) 46:546-555]

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adeno-associated viral vector-mediated human vascular endothelial growth factor gene transfer stimulates angiogenesis and wound healing in the genetically diabetic mouse

et al. 2003

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“…DOI: 10.1038/sj/gt/3301697 angiogenic growth factors from macrophages and keratinocytes and its impairment leads to a delay in skin repair. [7][8][9][10][11] One of the essential growth factors which are involved in the angiogenetic process in the wounded tissues is vascular endothelial growth factor (VEGF), 12 which promotes proliferation and migration of endothelial cells resulting in the formation of a capillary network which is an essential requisite for the healing process.…”

Section: Healing Is Concomitant With An Increase Of Production Of Andmentioning

confidence: 99%

Recombinant AAV vector encoding human VEGF165 enhances wound healing

et al. 2002

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“…Las proteínas HSP70 modulan el proceso de reacción inflamatoria (11) y son necesarias durante la cicatrización (14). De acuerdo a Oberringer y Atalay, su ausencia compromete el proceso reparativo inhibiéndolo o retrasándolo.…”

Section: Discussionunclassified

Ventajas de la cicatrización cutánea asistida por láser (LASH)

Mordon

Trelles²

2011

Cir. plást. iberolatinoam.

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ResumenLa fase final de toda cirugía es la sutura y su cicatriz correspondiente y, frecuentemente, ésta es la única secuela visible de una intervención. La técnica LASH (cicatrización cutánea asistida por láser), estimula los mecanismos de reparación de la piel por efecto térmico, condicionando la calidad de la cicatrización.Estudios experimentales en ratones comparando los resultados obtenidos en heridas quirúrgicas radiadas con laser diodo 810 nm tras ser suturadas, con los obtenidos en igual tipo de heridas suturadas de manera convencional, han demostrado que se consiguen cicatrices mucho menos visibles cuando se practica el tratamiento con láser.La histología confirma, comparativamente, una notable aceleración del proceso cicatricial con modificación del TGFß (Transforming Growth Factor-Beta) directamente implicado en la reparación tisular, en los animales en los que la sutura fue asistida por láser. Igualmente, el análisis tensiométrico demostró mayor resistencia a la acción de estiramiento mecánico en estas heridas.Estudios clínicos en pacientes sometidos a dermolipectomia, reducción mamaria y estiramiento facial, demuestran que al tratar con LASH una parte o una de las líneas de sutura, los análisis realizados tanto por los pacientes como por cirujanos independientes a los 8 días, 3 meses y un año, evidencian resultados más favorables para la técnica láser, Las dosis eficaces determinadas para las fluencias láser están entre 80 y 120 J/cm 2 .La técnica LASH es simple de efectuar, reproducible, rápida y ofrece una clara evidencia de que es posible reducir considerablemente la cicatriz, logrando que ésta sea de mejor calidad y más estética.Palabras clave Cicatrización, Láser.Cicatrización asistida, LASH. Código numérico 153-1545 AbstractSuture is the final stage of surgery and as a consequence, a scar is frequently the only visible effect after an operation. LASH technique ( Laser Assisted Scar Healing) produces thermal effects to stimulate mechanisms for tissue repair that will determine the quality of the scar.In the experimental phase, rats were irradiated with 810 nm diode-laser after surgical wounds were sutured, and then, compared to another group of rats with the same type of wounds which were sutured with conventional techniques. The results were that those rats treated with laser had less visible scars.Histology comparatively confirmed a notable acceleration in the scarring process with modification of the TGFß (Transforming Growth Factor-Beta), which is directly involved in tissue repair in those rats treated with laser. The tensiometric analysis also demonstrated a better resistance to mechanical stress of wounds.Clinically, in dermolipectomy patients, breast reductions and facial lifts, in which part of the sutures were treated with LASH, at 8 days, 3 months and 1 year controls, evaluated by independent surgeons to the study and by patients, statistical results were more favourable for patients treated with LASH technique. The effective doses determined for treatment were laser fluencies between...

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Differential Expression of Proteins during Healing of Cutaneous Wounds in Experimental Normal and Chronic Models

Cited by 75 publications

References 24 publications

Adeno-associated viral vector-mediated human vascular endothelial growth factor gene transfer stimulates angiogenesis and wound healing in the genetically diabetic mouse

Adeno-associated viral vector-mediated human vascular endothelial growth factor gene transfer stimulates angiogenesis and wound healing in the genetically diabetic mouse

Recombinant AAV vector encoding human VEGF165 enhances wound healing

Ventajas de la cicatrización cutánea asistida por láser (LASH)

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