Differential expression of skeletal muscle proteins in high‐fat diet‐fed rats in response to capsaicin feeding

Abstract

In this study, the effects of capsaicin on expression of skeletal muscle proteins in Sprague-Dawley rats fed with a high-fat diet (HFD) were investigated. Rats were fed a HFD with or without capsaicin treatment for 8 wk. After HFD feeding, capsaicin-treated rats weighed an average of 8% less than those of the HFD control group. Gastrocnemius muscle tissue from lean and obese rats with or without capsaicin treatment was arrayed using 2-DE for detection of HFD-associated markers. Proteomic analysis using 2-DE demonstrated that 36 spots from a total of approximately 600 matched spots showed significantly different expression; 27 spots were identified as gastrocnemius muscle proteins that had been altered in response to capsaicin feeding, and 6 spots could not be identified by mass fingerprinting. Expression of various muscle proteins was determined by immunoblot analysis for the determination of molecular mechanisms, whereby capsaicin caused inhibition of adipogenesis. Immunoblot analysis revealed increased uncoupling protein 3 (UCP3) protein expression in HFD-fed rats, whereas contents were reduced with capsaicin treatment. Compared with the HFD control group, capsaicin treatment increased phosphorylation of AMP-activated protein kinase (AMPIC) CP3 and acetyl-CoA carboxylase (ACC). To support this result, we also analyzed in vitro differential protein expression in L6 skeletal muscle cells. These data suggest that the AMPK-ACC-malonyl-CoA metabolic signaling pathway is one of the targets of capsaicin action. To the best of our knowledge, this is the first proteomic study to report on analysis of diet-induced alterations of protein expression that are essential for energy expenditure in rat muscle.

Anti-diabetic effects of Korean red pepper via AMPK and PPAR-γ activation in C2C12 myotubes

“…In addition, previous study suggested that dietary capsaicin improves insulin resistance by activating AMPK, suggesting that AMPK is important mediator for antidiabetic action (Kang et al, 2011). Another study also showed that administration of capsaicin significantly increased the AMPK activation and thereby resulted in stimulation of energy expenditure (Kim, Joo, Choi, & Yun, 2010). Therefore, we speculate that the capsaicin is also an important constituent for the anti-diabetic action of Korean red pepper.…”

The role of methylglyoxal and the glyoxalase system in diabetes and other age-related diseases

“…However, in red blood cells of obese mice, GLO1 activity was increased by 50-60 % compared with that of lean controls, and GLO2 activity rose by 20-30 % [75]. A proteomics study has demonstrated that GLO1 protein levels increase in the skeletal muscle of rats on a high-fat diet [178]. Furthermore, increased mRNA expression and activity of the GLO1 enzyme have been linked to mice that prefer high-carbohydrate diets [28].…”

Gender-Dimorphic Regulation of Skeletal Muscle Proteins in Streptozotocin-Induced Diabetic Rats

“…First, proteins were resolved on a broad-range IEF strip (pH 3-10, non-linear gradient) in the first dimension and on a 12% SDS-PAGE gel in the second dimension, as outlined by our previous studies [24,27]. However, improved resolution could be obtained when the first dimension separation was carried out on a pH 4-7 rather than pH 3-10 IEF strip.…”