Differential HSP70 gene expression in the Mediterranean mussel exposed to various stressors

Franzellitti, Silvia; Fabbri, Elena

doi:10.1016/j.bbrc.2005.08.244

Cited by 186 publications

(81 citation statements)

References 34 publications

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“…Organisms can be acclimated at the elevated temperature of 16°C after 2 months, thanks to the accumulation of new thermostable protein isoforms as suggested by Berger and Emlet (2007), and other inducible HSP70 isoforms or heat shock proteins can be sufficient to tolerate this temperature. Interestingly, this alternative is supported by Franzellitti and Fabbri (2005) suggestion that constitutive HSC70 are engaged in long-term protection, whereas HSP70s represent a short-term cytoprotective mechanism. 2.…”

Section: Discussionsupporting

confidence: 54%

“…Such mRNA HSP70 expression was highly similar to the transcriptional profile of heat-induced response observed in one species of Pacific abalone (Cheng et al 2007). This response might be considered atypical since it differed from several other studies in which mRNA levels were back to control levels much more rapidly, within 5-10 h after the heat shock (Bahrndorff et al 2009;Franzellitti and Fabbri 2005). N. rhenorhodanensis live in a thermally buffered biotope (groundwater) such as Orchesella cincta (soil; Bahrndorff et al 2009), whereas the Pacific abalone Haliotis discus hannai (Cheng et al 2007) and the mollusc M. galloprovincialis (Franzellitti and Fabbri 2005) live in highly fluctuating environments represented by intertidal biotopes.…”

Section: Discussionmentioning

confidence: 43%

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First cellular approach of the effects of global warming on groundwater organisms: a study of the HSP70 gene expression

Colson-Proch

Morales

Hervant

et al. 2010

Cell Stress and Chaperones

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Whereas the consequences of global warming at population or community levels are well documented, studies at the cellular level are still scarce. The study of the physiological or metabolic effects of such small increases in temperature (between +2°C and +6°C) is difficult because they are below the amplitude of the daily or seasonal thermal variations occurring in most environments. In contrast, subterranean biotopes are highly thermally buffered (±1°C within a year), and underground water organisms could thus be particularly well suited to characterise cellular responses of global warming. To this purpose, we studied genes encoding chaperone proteins of the HSP70 family in amphipod crustaceans belonging to the ubiquitous subterranean genus Niphargus. An HSP70 sequence was identified in eight populations of two complexes of species of the Niphargus genus (Niphargus rhenorhodanensis and Niphargus virei complexes). Expression profiles were determined for one of these by reverse transcription and quantitative polymerase chain reaction, confirming the inducible nature of this gene. An increase in temperature of 2°C seemed to be without effect on N. rhenorhodanensis physiology, whereas a heat shock of +6°C represented an important thermal stress for these individuals. Thus, this study shows that although Niphargus individuals do not undergo any daily or seasonal thermal variations in underground water, they display an inducible HSP70 heat shock response. This controlled laboratory-based physiological experiment constitutes a first step towards field investigations of the cellular consequences of global warming on subterranean organisms.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 43%

First cellular approach of the effects of global warming on groundwater organisms: a study of the HSP70 gene expression

Colson-Proch

Morales

Hervant

et al. 2010

Cell Stress and Chaperones

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“…Our hypothesis was verified as a particularly sustained stimulation of Hsp70 and a more transient stimulation of MT20 transcript levels was observed (Fig.7). The participation of Hsp70 in the cellular responses to stress in M. galloprovincialis has been documented in several previous studies (Snyder et al, 2001;Hamer et al, 2004;Malagoli et al, 2004;Franzellitti and Fabbri, 2005) and Hsp70 mRNA and metallothioneins content were found to be stimulated in gills from the bivalve Ostrea edulis after exposure to heat (Piano et al, 2004). Evidently, Hsp induction has long been demonstrated to constitute a ubiquitous mechanism which compensates for stressful conditions including osmotic perturbations, hypoxia and heavy metal exposure (Snyder et al, 2001), with different animals exhibiting diverse sensitivity thresholds and tissue-specific expression patterns (Hofmann, 1999).…”

Section: Discussionsupporting

confidence: 53%

Hyperthermia-induced Hsp70 and MT20 transcriptional upregulation are mediated by p38-MAPK and JNKs inMytilus galloprovincialis(Lamarck); a pro-survival response

Gourgou

Aggeli

Beis

et al. 2010

Journal of Experimental Biology

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SUMMARYIn the present study we investigated the signal transduction cascades triggered by acute thermal stress in Mytilus galloprovincialis gills. This particular species has been reported to exhibit a significant tolerance to high temperatures; thus, it was intriguing to examine the molecular mechanisms responsible for this extraordinary trait. In particular, exposure to 30°C was found to cause a significant and sustained stimulation of p38-MAPK phosphorylation while the activation profile of JNKs was transient and relatively moderate. We also observed that hyperthermia induced apoptosis as a delayed response, with both MAPK subfamilies rapidly translocating to the nucleus. The phosphorylation of cJun, ATF2 and NFB was detected next. Using selective inhibitors, phosphorylation of these transcription factors was established to be dependent on p38-MAPK or JNKs. Subsequently, potential changes in gene expression were assessed. In this context, hyperthermia resulted in the transcriptional upregulation of Hsp70 and MT20 genes with a widely known salutary effect, preserving mussel fitness and performance under adverse environmental conditions. Interestingly, p38-MAPK and JNKs were found to mediate the hyperthermia-induced Hsp70 and MT20 upregulation as well as the delayed induction of apoptosis under the interventions studied. Overall this is, to our knowledge, the first time that an insight into the compensatory survival 'programme' initiated in Mytilus galloprovincialis gills, contributing to this organism's exceptional tolerance to thermal stress, has been gained. In particular, we provide evidence demonstrating the principal role of p38-MAPK and JNKs in transducing the stress signal via mobilization of specific transcription factors and the transcriptional upregulation of cytoprotective genes.

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“…Intensities of immuno-reactive bands on blots from three independent experiments were measured and mean HSP70 expression levels calculated (b), with respect to control values represented by 1 and shown as the dotted line. **P≤ 0.01 when compared to DMSO controls (n=6, ±SEM) Discussion Aquatic organisms exhibit altered HSP expression when exposed to range of stressful conditions including seasonal acclimatisation, climate change (increase and decrease in temperature), competition for space, food availability, wave exposure, heavy metal exposure and laboratory adaptation (Buckley et al 2001;Franzellitti and Fabbri 2005;Helmuth et al 2002;Helmuth and Hofmann 2001;Kefaloyianni et al 2005;Lund et al 2006;Todgham et al 2007;Tomanek and Sanford 2003). For molluscs, HSP-like molecules have been identified in the sea slug, Aplysia californica, the oysters, Crassostrea virginica, O. edulis, and Crassostrea gigas, the mussel, Mytilus galloprovincialis, and the clams Tapes philippinarum and Scapharca inaequivalvis (Kuhl et al 1992;Piano et al 2002Piano et al , 2004Tirard et al 1995).…”

Section: Hsp70 Protein Reduction May Be Regulated By Effects Of Esps mentioning

confidence: 99%

Larval excretory-secretory products from the parasite Schistosoma mansoni modulate HSP70 protein expression in defence cells of its snail host, Biomphalaria glabrata

Zahoor

Davies²,

Kirk³

et al. 2010

Cell Stress and Chaperones

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Synthesis of heat shock proteins (HSPs) following cellular stress is a response shared by many organisms. Amongst the HSP family, the ∼70 kDa HSPs are the most evolutionarily conserved with intracellular chaperone and extracellular immunoregulatory functions. This study focused on the effects of larval excretory-secretory products (ESPs) from the parasite Schistosoma mansoni on HSP70 protein expression levels in haemocytes (defence cells) from its snail intermediate host Biomphalaria glabrata. S. mansoni larval stage ESPs are known to interfere with haemocyte physiology and behaviour. Haemocytes from two different B. glabrata strains, one which is susceptible to S. mansoni infection and one which is resistant, both showed reduced HSP70 protein levels following 1 h challenge with S. mansoni ESPs when compared to unchallenged controls; however, the reduction observed in the resistant strain was less marked. The decline in intracellular HSP70 protein persisted for at least 5 h in resistant snail haemocytes only. Furthermore, in schistosome-susceptible snails infected by S. mansoni for 35 days, haemocytes possessed approximately 70% less HSP70. The proteasome inhibitor, MG132, partially restored HSP70 protein levels in ESP-challenged haemocytes, demonstrating that the decrease in HSP70 was in part due to intracellular degradation. The extracellular signal-regulated kinase (ERK) signalling pathway appears to regulate HSP70 protein expression in these cells, as the mitogen-activated protein-ERK kinase 1/2 (MEK1/2) inhibitor, U0126, significantly reduced HSP70 protein levels. Disruption of intracellular HSP70 protein expression in B. glabrata haemocytes by S. mansoni ESPs may be a strategy employed by the parasite to manipulate the immune response of the intermediate snail host.

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Differential HSP70 gene expression in the Mediterranean mussel exposed to various stressors

Cited by 186 publications

References 34 publications

First cellular approach of the effects of global warming on groundwater organisms: a study of the HSP70 gene expression

First cellular approach of the effects of global warming on groundwater organisms: a study of the HSP70 gene expression

Hyperthermia-induced Hsp70 and MT20 transcriptional upregulation are mediated by p38-MAPK and JNKs inMytilus galloprovincialis(Lamarck); a pro-survival response

Larval excretory-secretory products from the parasite Schistosoma mansoni modulate HSP70 protein expression in defence cells of its snail host, Biomphalaria glabrata

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