Differential involvement of nitric oxide signaling in dopamine and PACAP stimulation of growth hormone release in goldfish

Novel function of recombinant pituitary adenylate cyclase-activating polypeptide as stimulator of innate immunity in African catfish (Clarias gariepinus) fry

“…In fish, studies on the relation between PACAP and nitric oxide production are scarce. Recently, the possible involvement of NOS/NO signaling in mediating PACAP stimulation of goldfish GH release [46] was investigated. The results showed that NOS/NO does not mediate PACAP stimulation of GH release [46].…”

Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: 20 Years after the Discovery

“…In fish, both PACAP and PRP stimulate GH secretion (Montero et al, 2000). In particular, PACAP provokes a robust increase of GH release from goldfish (Wong et al, , 2000Mitchell et al, 2008) and eel pituitary cells (Montero et al, 1998). PACAP increases intracellular calcium concentration in carp, frog, chicken, and rat somatotrope cells Gracia-Navarro et al, 1992;Yada et al, 1993;Scanes et al, 2007), and the PACAP-evoked calcium response is blocked by the PKA antagonist 3Ј,5Ј-cyclic monophosphorothioate, Rp-isomer, indicating that the effect of PACAP is mediated through activation of the cAMP/PKA pathway and subsequent activation of the Ca 2ϩ /calmodulin-dependent protein kinase cascade (Rawlings et al, 1993(Rawlings et al, , 1995Alarcón and García-Sancho, 2000;Wong et al, 2005).…”

Neuronal nitric oxide synthase in the olfactory system, forebrain, pituitary and retina of the adult teleost Clarias batrachus

“…While NADPHd positive cells were encountered in the pituitary of swordtail (Anken and Rahmann, 1996) and 10 species of catfish (Jadhao et al, 1999), NOS expression was detected in the pituitary of O. niloticus (Bordieri et al, 2003) and in the somatotropes of goldfish pituitary (Uretsky and Chang, 2000). While NO was found to be necessary for the GnRH induced GH secretion (Uretsky et al, 2003), dopamine stimulated GH release was mediated by NO in goldfish (Mitchell et al, 2008). Several immunoreactive cells and fibers were seen in the rostral pars distalis, proximal pars distalis and pars intermedia of the pituitary gland of C. batrachus.…”