Differential proteome analysis of mature and germinated embryos of Araucaria angustifolia

Abstract

Araucaria angustifolia is an endangered Brazilian native conifer tree. The aim of the present work was to identify differentially expressed proteins between mature and germinated embryos of A. angustifolia, using one and two dimensional gel electrophoresis approaches followed by protein identification by tandem mass spectrometry. The identities of 32 differentially expressed protein spots from two dimensional gel maps were successfully determined, including proteins and enzymes involved in storage mobilization such as the vicilin-like storage protein and proteases. A label free approach, based on spectral counts, resulted in detection of 10 and 14 mature and germinated enriched proteins, respectively. Identified proteins were mainly related to energetic metabolism pathways, translational processes, oxidative stress regulation and cellular signaling. The integrated use of both strategies permitted a comprehensive protein expression overview of changes in germinated embryos in relation to matures, providing insights into the this process in a recalcitrant seed species. Applications of the data generated on the monitoring and control of in vitro somatic embryos were discussed.

Biological activities and chemical constituents of Araucaria angustifolia : An effort to recover a species threatened by extinction

“…Currently, A. angustifolia is classified as vulnerable regarding the risk of extinction (Brazilian Decree 42,099, 2002). Due to this situation the cultivation of the species has received strong encouragement from governmental agencies related to environment and agriculture, and many efforts have been carried out in order to propagate and conserve the species (Balbuena et al, 2011;Vieira et al, 2012;Kuhn and Mariath, 2014).…”

pH do Exsudato na Avaliação da Viabilidade de Sementes de Araucaria angustifolia

“…é a única do gênero nativa do Brasil, com grande relevância ecológica, econômica e social na sua região de ocorrência natural (Schlögl et al, 2007). Por isso, diversos esforços têm sido conduzidos a fim de propagar e conservar a espécie (Balbuena et al, 2011). Na avaliação da qualidade fisiológica de sementes, o teste de germinação é amplamente utilizado para a maioria das espécies.…”

<b>Physiological and physical quality of local <i>Araucaria angustifolia</i> seed variety

“…In recent years, many studies have been conducted to examine seed development and/or somatic/zygotic embryogenesis of A. angustifolia (Balbuena et al, 2009;Agapito-Tenfen, Steiner, Guerra, & Nodari, 2011;Balbuena et al, 2011;Farias-Soares, Burrieza, Steiner, Maldonado, & Guerra, 2013;Rogge-Renner et al, 2013;Shibata, Coelho, & Steiner, 2013;Farias-Soares et al, 2014;Jo, Santos, Bueno, Barbosa, & Floh, 2014;Fraga, Vieira, Puttkammer, Oliveira, & Guerra, 2015;Steiner et al, 2015), and even though knowledge of the botanical varieties of this species has been reported since 1966 by Reitz and Klein (1966), little attention has been devoted to the characterization of these varieties. In this study, the results show a great diversity of the characteristics studied, and knowledge of varieties may contribute to their maintenance and regeneration.…”