Differential pulse polarographic determination of nicarbazin in chicken tissue

Abstract

An assay procedure for the quantitative determination of nicarbazin, which is an equimolar complex of 4,4'-dinitrocarbanilide (DNC) and 2-hydroxy-4,6-dimethylpyrimidine (HDP), in chicken muscle, liver, kidney, and skin-fat is described. The polarographically active moiety DNC is extracted from the tissue by solvent extraction and is determined quantitatively by differential pulse polarography. The estimated sensitivity of the

Liquid chromatographic tandem mass spectrometric determination of five coccidiostats in poultry eggs and feed

“…Most papers describing a method for the detection of nicarbazin in different matrices, use LC with UV detection [50][51][52][53][54][55][56][57][58][59][60][61][62][63][64]. Also papers describing LC-MS [4,5,59,[65][66][67][68][69], electrochemical methods [70,71] and pulse polarographic methods [70,72,73] are available.…”

A new and rapid method for the determination of nicarbazin residues in poultry feed, eggs and muscle tissue using supercritical fluid extraction and high performance liquid chromatography

“…Other methods include voltametric analysis by the Osteryoung square-wave technique 18 and differential pulse polarography. 19,20 The AOAC method 21 which is only specific for feeds uses a 30 g alumina column (500 3 22 mm id) with dimethylformamide (DMF) and alcohol as solvents for extraction followed by a clean-up procedure and finally spectrophotometric determination at 430 nm. The method is not ideal as it takes a long time (6 h), using large volumes of a potentially harmful solvent (DMF) and can only detect niacarbazin in feed (not eggs or muscle tissue) at levels of 25-125 ppm.…”

Modified pulse polarographic determination of nicarbazin in chicken tissue at the 0.1-ppm level

“…Chickens dosed with nicarbazin have been shown to excrete the DNC portion of the complex more slowly than the HDP portion (Porter and Gilfillan, 1955) and consequently all residue analyses for nicarbazin are based on methods for the DNC moiety. The official FDA method for nicarbazin residues in chicken currently involves pulse polarography of DNC (Michielli and Downing, 1974).…”