Differential regulation of the glucocorticoid receptor nucleocytoplasmic shuttling by TPR-domain proteins

Mazaira, Gisela Ileana; Echeverria, Pablo Christian; Ciucci, Sol M.; Monte, Martín; Gallo, Luciana I.; Erlejman, Alejandra G.; Galigniana, Mario D.

doi:10.1016/j.bbamcr.2021.119000

Cited by 13 publications

(8 citation statements)

References 77 publications

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“…For example, tetratricopeptide repeat (TPR) domains, which mediate protein-protein interactions ( Dermouche et al, 2021 ), Studies have shown that the C-terminal of Hsp90 dimer forms a functional receptor site for a co-partner carrying a tetratricopeptide repeat (TPR) domain, and they bind in a mutually exclusive or competitive manner. The biology of the glucocorticoid receptor (Hsp90•GR) complex is more potent, and it is a major player in the GR transport process ( Mazaira et al, 2021 ). Most MYB proteins act as transcription factors and have been shown to be involved in regulating various cellular processes, including cell cycle and morphogenesis, biological and abiotic stress responses ( Roy, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of Hsp40 and Hsp70 gene family in four cotton species provides insights into their involvement in response to Verticillium dahliae and abiotic stress

Zhou

Tang

et al. 2023

Front. Genet.

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Introduction: Cotton is an important economic crop to provide natural fibers as raw materials to textile industry, and is significantly affected by biotic and abiotic stress during the whole growth stage, in which Verticillium wilt (VW) caused by Verticillium dahliae is one of the most destructive disease to lead to a significant yield reduction. Heat shock proteins (Hsps) are important molecular chaperones, and play crucial roles in plant growth, development, resistance to biotic and abiotic stress. Hsp40 and Hsp70 are two key Hsps in cell chaperone network, however, the function and regulatory mechanism of Hsp40 and Hsp70 members in VW resistance and abiotic stress in cotton are largely unknown.Methods and Results: Herein, a systematic and comprehensive analysis of Hsp40s and Hsp70s in four cotton species of Gossypium arboretum, G. raimondii, G. hirsutum, and G. barbadense were performed. A total of 291 Hsp40s and 171 Hsp70s identified in four Gossypium species. Sequence analysis revealed that all Hsp40 proteins contained J domain that provides the binding sites to Hsp70. Protein-protein interaction prediction analysis displayed that GhHsp40-55 might interact with GhHsp70-2 and GhHsp70-13, suggesting their potential function as protein complex. Promoter cis-acting element analysis demonstrated that multiple cis-elements related to disease and stress response consists in GhHsp40 and GhHsp70 promoters. Further expression analysis showed that eight GhHsp40s (Hsp40-2,4,8,11,20,23,53,55) and seven GhHsp70s (Hsp70-2,3,6,8,13,19,22) were up-regulated after V. dahliae infection. In addition, five GhHsp40s (Hsp40-2,8,11,53,55) and four GhHsp70s (Hsp70-3,6,8,13) were up-regulated after salt treatment, six GhHsp40s (Hsp40-4,11,20,23) and three GhHsp70s (Hsp70-2,8,19) were up-regulated after drought treatment, four GhHsp40s (Hsp40-2,11,20,23) and four GhHsp70s (Hsp70-3,6,19,22) were up-regulated after temperature treatment, suggesting these Hsps have possible important function in the process of abiotic stress response.Discussion: Our results lay a foundation for understanding the function of Hsp40 and Hsp70 in the resistance against V. dahliae and abiotic stress, and elucidating the regulatory mechanism of the protein complex, evolution and molecular mechanism under stress.

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Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of Hsp40 and Hsp70 gene family in four cotton species provides insights into their involvement in response to Verticillium dahliae and abiotic stress

Zhou

Tang

et al. 2023

Front. Genet.

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“…Notably, all three of these co-chaperones can be in complex with the co-chaperone PP5 (Woodford et al, 2016(Woodford et al, , 2017, a protein phosphatase that dephosphorylates and inactivates GR (Wang et al, 2007;Zuo et al, 1999). The Hsp90 co-chaperones FKBP51 and -52 are also specialized SHR co-chaperones that regulate SHR maturation and play a role in translocation of ligand-bound SHR to the nucleus (Guy et al, 2015;Mazaira et al, 2021;Riggs et al, 2007). While it remains unknown whether FNIPs and Tsc1 are important for subcellular localization of SHR, the current understanding of SHR activation would suggest that all components of the complex present at the time of ligand binding localize to the nuclear membrane.…”

Section: Discussionmentioning

confidence: 99%

A specialized Hsp90 co-chaperone network regulates steroid hormone receptor response to ligand

et al. 2022

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“…Another study observed high nuclear GR signal in dorsal CA1 and dentate gyrus only in rats that were strongly affected by predator scent exposure ( Danan et al, 2021 ). While the presence of GR nuclear generally follows corticosterone levels, additional regulatory mechanisms are governing nuclear translocation ( Mazaira et al, 2021 ), and these may be relevant to the brain as evidenced by the nuclear GR localization even in adrenalectomized rats ( Sarabdjitsingh et al, 2009 ). FKBP5 is an often studied factor in this respect, i.e., both target and regulator of GR function ( Binder, 2009 ; Matosin et al, 2018 ; Häusl et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

An Advanced Transcriptional Response to Corticosterone After Single Prolonged Stress in Male Rats

Ding

Xinzhao

Han

et al. 2021

Front. Behav. Neurosci.

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Stress-related neuropsychiatric disorders are often accompanied by dysfunction of the hypothalamic-pituitary-adrenal (HPA) axis. In patients suffering from post-traumatic stress disorder (PTSD), increased sensitivity of glucocorticoid negative feedback has regularly been observed. The single prolonged stress (SPS) paradigm was developed to model increased negative feedback and other aspects of PTSD in rats. In this study, we used a setup that precluded the evaluation of negative feedback but rather served to test the hypothesis of the enhanced glucocorticoid receptor (GR) signaling in higher brain areas. We injected corticosterone or vehicle 7 days after SPS and evaluated plasma corticosterone, as well as gene expression in the dorsal hippocampus and amygdala. We observed a strikingly rapid change in the expression of established GR target genes (t = 30 min) only in the SPS group on exogenous corticosterone injection. Our results extend the notion of increased GR sensitivity in PTSD to include transcriptional responses in the hippocampus.

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Differential regulation of the glucocorticoid receptor nucleocytoplasmic shuttling by TPR-domain proteins

Cited by 13 publications

References 77 publications

Genome-wide analysis of Hsp40 and Hsp70 gene family in four cotton species provides insights into their involvement in response to Verticillium dahliae and abiotic stress

Genome-wide analysis of Hsp40 and Hsp70 gene family in four cotton species provides insights into their involvement in response to Verticillium dahliae and abiotic stress

A specialized Hsp90 co-chaperone network regulates steroid hormone receptor response to ligand

An Advanced Transcriptional Response to Corticosterone After Single Prolonged Stress in Male Rats

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