Differential scanning calorimetry to study the possible ternary complex formation between chlorhexidine, phosphatidylcholine and some nonionic tenzides

Dentin matrix metalloproteinases (MMPs) are involved in collagen degradation of resin-dentin interfaces. This study evaluated if collagen degradation can be prevented by chlorhexidine after different dentin demineralization procedures. Human dentin demineralization was performed with phosphoric acid (PA), EDTA, or acidic monomers (ClearfilSEBond and XENOV). Specimens were stored (24 h, 1 wk or 3 wk) in the presence or absence of chlorhexidine. In half of the groups, active MMP-2 was incorporated into the storing solution. C-terminal telopeptide determination (ICTP) was performed in the supernatants. Collagen degradation was higher in PA and EDTA-demineralized dentin. Chlorhexidine reduced collagen degradation in these groups only for 24 h. When dentin was demineralized with SEBond or Xeno, collagen degradation was reduced up to 30%, but addition of exogenous MMP-2 significantly increased collagen degradation. In self-etchant treated dentin the inhibitory effect of chlorhexidine on MMPs lasted up to 3 wk. Treating dentin with EDTA, PA or self-etching agents produces enough demineralization to permit cleavage of the exposed collagen. Monomers infiltration may exert protection on demineralized collagen, probably through immobilization of MMPs. The partial inhibitory action of CHX on MMP activity produced by self-etching adhesives was prolonged compared to the short-acting in PA or EDTA-treated dentin.

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“…Thus, clearance of CHX from monomers may have occurred. Detergents (42) and non-ionic chemical cleaners are known to deactivate CHX (43).…”

Section: Discussionmentioning

confidence: 99%

Effect of dentin etching and chlorhexidine application on metalloproteinase‐mediated collagen degradation

Osorio

Yamauti

Osorio

et al. 2011

European J Oral Sciences

154

137

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Non‐linear mapping for structure‐activity and structure‐property modelling

Domine

Devillers²,

Chastrette

et al. 1993

Journal of Chemometrics

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SUMMARYFrom a review of the theoretical aspects of non-linear mapping and the different algorithms available in the literature, the methodological and practical problems linked to the use of this multivariate method in structure-activity and structure-property relationship studies are underlined. Useful tools for the graphical display of the outputs and the interpretation of the obtained clusters are presented. Statistical parameters estimating the quality of the graphical representation of each individual are also introduced. An example of application on a data matrix of 37 acaricides described by four physicochemical descriptors (T, F, R, M R ) is presented.

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Interaction of monoamine oxidase inhibitory drugs with some phospholipids

Szőgyi

Cserháti

1993

Journal of Pharmaceutical and Biomedical Analysis

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Differential scanning calorimetry to study the possible ternary complex formation between chlorhexidine, phosphatidylcholine and some nonionic tenzides

Cited by 3 publications

References 18 publications

Effect of dentin etching and chlorhexidine application on metalloproteinase‐mediated collagen degradation

Effect of dentin etching and chlorhexidine application on metalloproteinase‐mediated collagen degradation

Non‐linear mapping for structure‐activity and structure‐property modelling

Interaction of monoamine oxidase inhibitory drugs with some phospholipids

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