Differential Spread of Potato virus Y (PVY) Strains O, N:O and NTN in the Field: Implications for the Rise of Recombinant PVY Strains in New Brunswick, Canada

MacKenzie, Tyler D. B.; Lavoie, Jacques; Nie, Xianzhou; Singh, Mathuresh

doi:10.1007/s12230-018-9632-6

Cited by 27 publications

(4 citation statements)

References 37 publications

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“…Recombination, together with mutation, increases the genetic variability of the given taxa, which can result in higher viral fitness or survival of the virus population in a previously nonviable environment, sometimes leading to the emergence of new resistance-breaking strains [36,37]. Indeed, a change in the PVY strain prevalence from a non-recombinant to recombinant one during the last few decades was generally observed [6,38,39] suggesting a better adaptation of current recombinant strains over non-recombinant ones. Similarly, the latest reports of PVY strain characterization in infected plants point to a growing incidence of recombinant PVY strains [40].…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Potato Virus Y (PVY) Using High-Throughput Sequencing: Constraints on Full Genome Reconstructions Imposed by Mixed Infection Involving Recombinant PVY Strains

Glasa

Hančinský

Šoltýs

et al. 2021

Plants

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In recent years, high throughput sequencing (HTS) has brought new possibilities to the study of the diversity and complexity of plant viromes. Mixed infection of a single plant with several viruses is frequently observed in such studies. We analyzed the virome of 10 tomato and sweet pepper samples from Slovakia, all showing the presence of potato virus Y (PVY) infection. Most datasets allow the determination of the nearly complete sequence of a single-variant PVY genome, belonging to one of the PVY recombinant strains (N-Wi, NTNa, or NTNb). However, in three to-mato samples (T1, T40, and T62) the presence of N-type and O-type sequences spanning the same genome region was documented, indicative of mixed infections involving different PVY strains variants, hampering the automated assembly of PVY genomes present in the sample. The N- and O-type in silico data were further confirmed by specific RT-PCR assays targeting UTR-P1 and NIa genomic parts. Although full genomes could not be de novo assembled directly in this situation, their deep coverage by relatively long paired reads allowed their manual re-assembly using very stringent mapping parameters. These results highlight the complexity of PVY infection of some host plants and the challenges that can be met when trying to precisely identify the PVY isolates involved in mixed infection.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Potato Virus Y (PVY) Using High-Throughput Sequencing: Constraints on Full Genome Reconstructions Imposed by Mixed Infection Involving Recombinant PVY Strains

Glasa

Hančinský

Šoltýs

et al. 2021

Plants

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“…A similar situation is also common in other countries. Reports are focused on the monitoring of one or two pathogens within the whole country (see, for example, References [19,20,21,22]), and only a few studies describe the screening of three or four viral or bacterial pathogens in certain areas or small countries [23,24]. As far as we know, detailed information about the presence of the major potato pathogens in the majority of potato-producing regions of a country was reported only for viruses in China [25,26], Tunisia [27], and Pakistan [28] by including information about the presence and composition of mixed viral infections [26,28].…”

Section: Introductionmentioning

confidence: 99%

Potato Pathogens in Russia’s Regions: An Instrumental Survey with the Use of Real-Time PCR/RT-PCR in Matrix Format

Malko¹,

Frantsuzov²,

Nikitin³

et al. 2019

Pathogens

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Viral and bacterial diseases of potato cause significant yield loss worldwide. The current data on the occurrence of these diseases in Russia do not provide comprehensive understanding of the phytosanitary situation. Diagnostic systems based on disposable stationary open qPCR micromatrices intended for the detection of eight viral and seven bacterial/oomycetal potato diseases have been used for wide-scale screening of target pathogens to estimate their occurrence in 11 regions of Russia and to assess suitability of the technology for high-throughput diagnostics under conditions of field laboratories. Analysis of 1025 leaf and 725 tuber samples confirmed the earlier reported data on the dominance of potato viruses Y, S, and M in most regions of European Russia, as well as relatively high incidences of Clavibacter michiganensis subsp. sepedonicus, Pectobacterium atrosepticum, and P. carotovorum subsp. carotovorum, and provided detailed information on the phytosanitary status of selected regions and geographical spread of individual pathogens. Information on the occurrence of mixed infections, including their composition, was the first data set of this kind for Russia. The study is the first large-scale screening of a wide range of potato pathogens conducted in network mode using unified methodology and standardized qPCR micromatrices. The data represent valuable information for plant pathologists and potato producers and indicate the high potential of the combined use of matrix PCR technology and network approaches to data collection and analysis with the view to rapidly and accurately assess the prevalence of certain pathogens, as well as the phytosanitary state of large territories.

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“…In this study, PVY infection occurred symptomless in some of the most commonly grown cultivars. Previous studies indicated that such symptomless infections are one of the factors that have contributed to the rapid spread of PVY N-Wi in different potato growing regions all over the world by invisibly acting as a source of PVY inoculum (Karasev and Gray 2013;MacKenzie et al 2018). Symptomless PVY infections were found in cultivars Shangi, Tigoni, Kerr's Pink, Kenya Baraka and Asante both in the screen-house and in the field screening of cultivars over seasons after inoculation with the PVY strain.…”

Section: Discussionmentioning

confidence: 99%

Screening for PVYN-Wi Resistance in Kenyan Potato Cultivars

2021

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Over the past decades, two new strains of potato virus Y (PVYN-Wi (Wilga) and PVYNTN) have been the most widely distributed worldwide in potato crops (Solanum tuberosum L.). Effective resistance genes comprehensive against all the known strains of the virus are available in some commercial cultivars. However, not all resistance genes in cultivars have been challenged by the new PVY strains to confirm effectiveness of the resistances available. This study was set to identify PVY resistant genotypes and estimate yield losses in potato cultivars in Kenya. PVYN-Wi was used for PVY resistance screening because it was the most prevalent strain in Kenya and also was found to produce more severe infection in Kenyan potato genotypes compared with PVYO (the second most prevalent strain). Three cultivars, Kenya Karibu, Sherekea and Unica, were identified as resistant because they did not get PVY infections over three seasons of both greenhouse and field screening experiments. In addition, two early generation breeding clones were identified as resistant at 28 days post-inoculation. In susceptible cultivars Dutch Robyjn, Nyayo and Kenya Mpya, PVYN-Wi infection caused yield losses of 21.6, 39.0 and 53.1%, respectively, after three seasons. This study demonstrated the necessity to screen for PVY resistance based on the most prevalent PVY strains and to make an informed decision on the usefulness of current levels of resistance in potato cultivars.

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Differential Spread of Potato virus Y (PVY) Strains O, N:O and NTN in the Field: Implications for the Rise of Recombinant PVY Strains in New Brunswick, Canada

Cited by 27 publications

References 37 publications

Molecular Characterization of Potato Virus Y (PVY) Using High-Throughput Sequencing: Constraints on Full Genome Reconstructions Imposed by Mixed Infection Involving Recombinant PVY Strains

Molecular Characterization of Potato Virus Y (PVY) Using High-Throughput Sequencing: Constraints on Full Genome Reconstructions Imposed by Mixed Infection Involving Recombinant PVY Strains

Potato Pathogens in Russia’s Regions: An Instrumental Survey with the Use of Real-Time PCR/RT-PCR in Matrix Format

Screening for PVYN-Wi Resistance in Kenyan Potato Cultivars

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