Differentially Expressed Genes of Soybean During Infection by Phytophthora sojae

Xu, Pengfei; Wu, Jianzhong; Xue, Allen; Li, Wenbin; Chen, Weiyuan; Wei, Lai; Lv, Huiying; Lin, Shan; Fan, Shiliang; Li, Ninghui; Wang, Xin; Jiang, Lan; Zhang, Shuzhen

doi:10.1016/s2095-3119(12)60021-5

Cited by 24 publications

(31 citation statements)

References 42 publications

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“…We previously reported that GmNPR1 was induced after infection with the oomycete P. sojae (Xu et al, 2012). To determine whether GmNPR1 is involved in GmWRKY31 -overexpression-mediated defense pathways in soybean, we analyzed GmNPR1 mRNA levels in GmWRKY31 transgenic soybean plants by qRT-PCR.…”

Section: Resultsmentioning

confidence: 99%

“…In Arabidopsis, WRKY TFs play a crucial role in the response to pathogen infection (Abuqamar et al, 2006; Journot-Catalino et al, 2006; Li et al, 2006; Xu et al, 2006; Knoth et al, 2007; Lippok et al, 2007; Murray et al, 2007; Zheng et al, 2007; Hu et al, 2008; Mao et al, 2011). Overexpression of AtWRKY70 leads to the up-regulation of PR genes and the down-regulation of PDF1.2 , leading to enhanced resistance against biotrophic pathogens and increased susceptibility to constitutive necrotrophic pathogens (Li et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean

et al. 2017

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Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.] caused by the oomycete Phytophthora sojae, is a destructive disease worldwide. The molecular mechanism of the soybean response to P. sojae is largely unclear. We report a novel WRKY transcription factor (TF) in soybean, GmWRKY31, in the host response to P. sojae. Overexpression and RNA interference analysis demonstrated that GmWRKY31 enhanced resistance to P. sojae in transgenic soybean plants. GmWRKY31 was targeted to the nucleus, where it bound to the W-box and acted as an activator of gene transcription. Moreover, we determined that GmWRKY31 physically interacted with GmHDL56, which improved resistance to P. sojae in transgenic soybean roots. GmWRKY31 and GmHDL56 shared a common target GmNPR1 which was induced by P. sojae. Overexpression and RNA interference analysis demonstrated that GmNPR1 enhanced resistance to P. sojae in transgenic soybean plants. Several pathogenesis-related (PR) genes were constitutively activated, including GmPR1a, GmPR2, GmPR3, GmPR4, GmPR5a, and GmPR10, in soybean plants overexpressing GmNPR1 transcripts. By contrast, the induction of PR genes was compromised in transgenic GmNPR1-RNAi lines. Taken together, these findings suggested that the interaction between GmWRKY31 and GmHDL56 enhances resistance to P. sojae by regulating defense-related gene expression in soybean.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean

et al. 2017

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“…A suppression subtractive hybridization library coupled with cDNA microarrays was queried using a soybean expressed sequence tag (EST) encoding an EST homologous to a phenylalanine ammonia-lyase from Lotus japonicus , previously shown to be upregulated in the highly resistant soybean ‘Suinong 10’ infected with P . sojae 46 . Here, the full-length cDNA (termed GmPAL2 .…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study, a cDNA library enriched for mRNAs encoding ESTs that were increased in abundance during infection with P . sojae was constructed by suppression subtractive hybridization from leaf tissues of the highly resistant soybean cultivar ‘Suinong 10’, and an EST homologous to a phenylalanine ammonia-lyase from Lotus japonicus was identified to be upregulated by microarray and real-time PCR 46 . In the present work, the full-length EST, designated GmPAL2 .…”

Section: Introductionmentioning

confidence: 99%

Phenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection

Zhang

Wang

Zhang

et al. 2017

Sci Rep

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Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.] caused by Phytophthora sojae is a destructive disease worldwide. Phenylalanine ammonia-lyase (PAL) is one of the most extensively studied enzymes related to plant responses to biotic and abiotic stresses. However, the molecular mechanism of PAL in soybean in response to P. sojae is largely unclear. Here, we characterize a novel member of the soybean PAL gene family, GmPAL2.1, which is significantly induced by P. sojae. Overexpression and RNA interference analysis demonstrates that GmPAL2.1 enhances resistance to P. sojae in transgenic soybean plants. In addition, the PAL activity in GmPAL2.1-OX transgenic soybean is significantly higher than that of non-transgenic plants after infection with P. sojae, while that in GmPAL2.1-RNAi soybean plants is lower. Further analyses show that the daidzein, genistein and salicylic acid (SA) levels and the relative content of glyceollins are markedly increased in GmPAL2.1-OX transgenic soybean. Taken together, these results suggest the important role of GmPAL2.1 functioning as a positive regulator in the soybean response to P. sojae infection, possibly by enhancing the content of glyceollins, daidzein, genistein and SA.

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“…As a result, many studies performed on M. truncatula, soybean, pea, chickpea, peanuts, and common bean provided insight into the molecular basis of their resistance to several legume pathogens including Fusarium oxysporum, Aphanomyces euteiches, Phytophthora sojae, Didymella pinodes, Ascochyta rabiei, Phakopsora pachyrhizi, Peronospora viciae f.sp. pisi, pea seed-borne mosaic virus, bean common mosaic virus, common bacterial blight, Orobanche crenata, Phelipanche aegyptiaca, Heterodera goettingiana, Spodoptera exigua or Helicoverpa armigera (Nyamsuren et al, 2003;Gao et al, 2004;Cho et al, 2005;Cadle-Davidson and Jahn, 2006;Nimbalkar et al, 2006;Die et al, 2007;Choi et al, 2008;Darwish et al, 2008;Singh et al, 2008;Gupta et al, 2009Gupta et al, , 2010Hiraoka et al, 2009;Soria-Guerra et al, 2010;Shi et al, 2011;Veronico et al, 2011;Feng et al, 2012;Huang et al, 2012a,b;Jaiswal et al, 2012;Xu et al, 2012;Rispail et al, 2013;Toyoda et al, 2013).…”

Section: B Transcriptomicsmentioning

confidence: 99%

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Rubiales

Fondevilla

Cao

et al. 2014

Critical Reviews in Plant Sciences

184

132

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Differentially Expressed Genes of Soybean During Infection by Phytophthora sojae

Cited by 24 publications

References 42 publications

GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean

GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean

Phenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

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