Differentially expressed microRNAs in peripheral blood mononuclear cells of non‐segmental vitiligo and their clinical significance

Zhang, Zhibin; Yang, Xiongwen; Liu, Ougen; Cao, Xianwei; Tong, Jianbo; Xie, Ting; Zhang, Jie; Peng, Yating

doi:10.1002/jcla.23648

Cited by 16 publications

(13 citation statements)

References 32 publications

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“…31 Many miRNAs are dysregulated in nonsegmental vitiligo 32,33 and have been shown to target major pigmentation genes like Tyr and MITF. 34 It has been shown that divergently expressed miRNAs contribute to the pathogenesis of nonsegmental vitiligo 35 ; for example, miRNA-145 has been shown to have a key role in regulating melanogenesis as suggested by a number of studies. 22,36 As far as we know, miRNA-377 expression hasn't been estimated in vitiligo before.…”

Section: Discussionmentioning

confidence: 99%

Association between long noncoding RNA taurine‐upregulated gene 1 and microRNA‐377 in vitiligo

et al. 2021

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Background Taurine-upregulated gene 1 (TUG1) is one of the long noncoding RNAs (lncRNAs) that plays a role in melanogenesis. is a conserved noncoding RNA that regulates angiogenesis and promotes oxidative stress. Peroxisome proliferator-activated receptors (PPARs) are components of the nuclear hormone receptor superfamily. PPAR-c activators stimulate melanogenesis. Interleukin (IL)-17 has been implicated in the pathogenesis of several immunological diseases. This work aimed at detecting the expression levels of lncRNA TUG1, miRNA-377, PPAR-c, and IL-17 among vitiligo subjects and to investigate their possible role in the pathogenesis of vitiligo.Methods This study was conducted on 30 healthy controls and 30 vitiligo patients.LncRNA TUG1 and miRNA-377 were detected in serum by real-time polymerase chain reaction (PCR). Also, expressions of PPAR-c and IL-17 were assessed in tissue by realtime PCR.Results LncRNA TUG1 and PPAR-c levels were significantly downregulated in the vitiligo group compared with the control group. On the other hand, miRNA-377 and IL-17 were significantly upregulated in the vitiligo group compared with the control group. ConclusionThis study demonstrated the dysregulated expressions of lncRNA TUG1 and miRNA-377 in patients with vitiligo suggesting that both contributed to the pathogenesis of vitiligo that might be through PPAR-c downregulation and IL-17 upregulation.

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Section: Discussionmentioning

confidence: 99%

Association between long noncoding RNA taurine‐upregulated gene 1 and microRNA‐377 in vitiligo

et al. 2021

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“…We explored the correlation between the expression of miRNAs and the area of lesions by Spearman correlation coefficient using IBM ® SPSS Statistics Software (V27.0). 23 …”

Section: Methodsmentioning

confidence: 99%

Differential Expression of Serum Exosomal Hsa-miR-487b-3p in Progressive Vitiligo Before and After Systemic Corticosteroid Treatment

Luo

Xie

et al. 2022

CCID

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Background Vitiligo is an acquired skin depigmentation disease. It can be misdiagnosed at an early stage and tend to relapse. Serum markers are essential to monitoring the progression of vitiligo. Exosomal miRNAs act as the communication mediator between melanocytes and immune cells. Our study aimed to use serum exosomal miRNAs as a reference for evaluating vitiligo progression. Methods The miRNAs were extracted from the serum exosomes of ten progressive vitiligo patients (before and after treatment) and ten healthy individuals. We profiled miRNAs expression by RNA sequencing and screened out potential miRNAs and plotted their receiver operating characteristic (ROC) curves to explore their sensitivity and specificity as prognostic biomarkers in vitiligo progression. We examined the correlation between miRNA expression and the lesion area. Different databases were used to predict gene targets of miRNAs, which were analyzed by gene ontology and Kyoto encyclopedia of genes and genomes (KEGG). Results Our results showed that 141 miRNAs were differentially expressed in serum exosomes of progressive vitiligo patients, and 365 miRNAs were differentially expressed in these patients after treatment compared to healthy individuals. The expression of hsa-miR-487b-3p was significantly lower in these patients compared to healthy individuals. Still, there was no difference in its levels in patients after corticosteroid treatment compared to healthy controls. ROC curve analysis (area under curve = 0.840) indicated that hsa-miR-487b-3p could serve as a biomarker for the prognosis of vitiligo progression. Its expression positively correlated with the lesion area. A total of 41 target genes of hsa-miR-487b-3p were predicted via different databases. KEGG pathways were enriched in phenylalanine metabolism, glycan degradation, and protein export. Conclusion Serum exosomal hsa-miR-487b-3p can be a biomarker to detect vitiligo progression. The predicted target genes of hsa-miR-487b-3p were enriched in catabolism. Thus, its in progressive vitiligo may accelerate catabolism in melanocytes and cause its impairment.

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“…Another study also analyzed miRNA expression profile in the PBMCs of NSV patients (Zhang et al, 2020). The top 10 upregulated miRNAs included miR- 335-5p, miR-20a-5p, miR-514a-3p, miR-144-5p, miR-450b-5p, miR-369-3p, miR-101-3p, miR-142-5p, miR-19b-3p, and miR-340-5p.…”

Section: Altered Mirna Expression In Peripheral Blood Mononuclear Cellsmentioning

confidence: 99%

MicroRNAs: Emerging players in the pathogenesis of vitiligo

Cui

Zhu

et al. 2022

Front. Cell Dev. Biol.

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Vitiligo is an autoimmune skin disease characterized by presence of pale patchy areas of depigmentation. MicroRNAs (miRNAs) are important regulators of gene expression and play significant roles in diverse biological and pathological processes. Accumulating evidence has shown that miRNAs were differentially expressed in skin lesions and peripheral blood mononuclear cells of patients with vitiligo. In particular, miRNAs are significantly correlated with the development and progression of vitiligo. The abundance of some miRNAs in serum was also correlated with the vitiligo lesion severity, indicating that miRNAs might serve as prognostic biomarkers. Importantly, the direct involvement of miRNAs in the pathogenesis of vitiligo has been demonstrated. For example, increased expression of miR-25 contributes to vitiligo through promoting the dysfunction and oxidative stress-induced destruction of melanocytes. However, there are limited studies on the function and mechanism of deregulated miRNAs in vitiligo. Further studies are required to establish clinical applications of miRNAs for vitiligo. More in-depth investigations of miRNAs are needed for the understanding of the pathogenesis of vitiligo and the development of novel therapeutic targets. This present review summarizes the current literature on the deregulation and pathogenic roles of miRNAs in vitiligo. We also highlight the potential clinical applications of miRNAs in patients with vitiligo.

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Differentially expressed microRNAs in peripheral blood mononuclear cells of non‐segmental vitiligo and their clinical significance

Cited by 16 publications

References 32 publications

Association between long noncoding RNA taurine‐upregulated gene 1 and microRNA‐377 in vitiligo

Association between long noncoding RNA taurine‐upregulated gene 1 and microRNA‐377 in vitiligo

Differential Expression of Serum Exosomal Hsa-miR-487b-3p in Progressive Vitiligo Before and After Systemic Corticosteroid Treatment

MicroRNAs: Emerging players in the pathogenesis of vitiligo

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