Differentiation of bacterial spores via 2D-IR spectroscopy

Biomolecular infrared spectroscopy: making time for dynamics

“…94,96,142 The eld should embrace such opportunities as they bring new challenges which ultimately drive technique development, but also broaden the user base for ultrafast IR capability which will accelerate integration of the methods within the biomolecular research community. A viable route through which this may proceed is offered by proof of concept studies in cells 93,143,144 and the development of multidimensional IR imaging modalities. 145,146 One area that has the potential to signicantly enhance the reach and impact of long timescale IR spectroscopies is the interaction with computational simulations.…”

Fourier Transform Infrared (FTIR) Spectroscopic Study of Biofilms Formed by the Rhizobacterium Azospirillum baldaniorum Sp245: Aspects of Methodology and Matrix Composition

“…The diverse field of microbiology has been progressively developing over the last decades, largely owing to the steadily growing applications of a range of modern molecular spectroscopy techniques that provide molecular-level information on complicated microbiological objects and allow for successfully solving various bioanalytical problems, often in situ or in vivo (see, e.g., [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ]). We would like to emphasize the informativity of very relevant vibrational spectroscopic techniques, including various modifications of infrared (conventional absorption [ 2 , 7 , 11 , 16 ], diffuse reflectance [ 3 , 6 ], attenuated total reflectance (ATR) [ 5 , 17 ] and surface-enhanced absorption [ 15 ] modes; 2D infrared [ 8 ]) and Raman [ 1 , 2 , 13 ] spectroscopies, as well as their combinations [ 4 , 14 ]; vibrational circular dichroism [ 12 ], optical activity [ 9 ], etc. Of special notice are microbiological applications of Mössbauer spectroscopy, in both transmission and emission variants [ 2 , 10 ].…”

“…Among these techniques, Fourier transform infrared (FTIR) spectroscopy, which has already become a routine tool in material science, yet could not be regarded as routine in the life sciences [ 4 , 6 , 7 ], and particularly in microbiology [ 3 , 5 , 8 , 13 , 14 , 15 , 17 , 18 , 19 ]. This situation can in part be explained by the intrinsically sophisticated compositions and structures of diverse microbiological objects, making the processes of sample treatment or preparation for spectroscopic studies, together with treatment of the spectroscopic data and interpreting the results, a challenging task [ 19 ].…”

“…Moreover, the overall cell composition can also rapidly alter depending on the growth phase and/or in response to the changing environments. This complexity is probably the main reason that, despite a great number of studies involving FTIR spectroscopy in microbiology reported so far (see, e.g., [ 3 , 5 , 8 , 14 , 15 , 17 , 18 , 19 ]), the available standardised protocols for FTIR spectroscopic microbiological analyses (including sample preparation) are insufficient, not yet fully generalised and cannot cover all possible cases [ 19 , 20 , 21 , 22 ]. Thus, in the review chapter by Ojeda and Dittrich [ 19 ], a general overview was presented covering the different sample preparation protocols for infrared spectroscopic analysis of bacterial cells, the basic principles of the technique, procedures for calculating vibrational frequencies based on simple harmonic motions, as well as the advantages and disadvantages of FTIR spectroscopy for the analysis of microorganisms.…”