Differentiation of bursal secretory‐dendritic cells studied with anti‐vimentin monoclonal antibody

Abstract

Embryonic and posthatched differentiation of bursal secretory dendritic cells, which express vimentin intermediate filaments, were studied with anti-vimentin (clone 3B4) and anti-cytokeratin (clone Lu5) monoclonal antibodies. Anti-cytokeratin staining revealed that medullary reticular epithelial cells formed a continuous network at every age, whereas the vimentin positive cells were single and showed dendritic appearance. On the basis of location, number, shape, polarized appearance, and Ia staining, the vimentin-positive cells and secretory dendritic cells appeared to be the same cell. Secretory dendritic cell precursors entered the bursal epithelium between 11 and 13 days of embryogenesis. The first vimentin positive cell appeared in the bud of 14-day embryos. Bud formation preceded the appearance of vimentin-positive cells. These observations suggested that the secretory dendritic cell precursor did not express vimentin when it entered the epithelium. Between 15 days of embryogenesis and 2 weeks of posthatch development, the changes in vimentin staining pattern revealed a cytological differentiation of the vimentin-positive cell. During rapid bursal growth, the number of secretory dendritic cells (vimentin-positive cells) increased about 18 times possibly by proliferation of vimentin-negative precursors in the epithelial arches of the corticomedullary border.

Expression of Chicken DEC205 Reflects the Unique Structure and Function of the Avian Immune System

“…Its distribution closely resembles that of vimentin expressing BSDC previously described by Olah and Glick [35]–[37]. A minor population of DEC205 +ve cells with dendritic morphology are dispersed throughout both medulla and cortex.…”

Immunoreactive POMC‐Derived Peptides and Cytokines in the Chicken Thymus and Bursa of Fabricius Microenvironments: Age‐Related Changes

“…Immunocytochemical procedures were carried out as previously described in detail ( 10) on sections prepared as for histological analysis. The following polyclonal (pAb) and monoclonal (mAb) antibodies were used: rabbit anti‐synthetic adrenocorticotropic hormone ( 1–24) (ACTH) pAb (Biogenesis, Poole, UK), (1:1000); rabbit anti‐synthetic β ‐endorphin pAb (Sera‐lab, UK) (1:2000); rabbit anti‐synthetic α ‐melanocyte‐stimulating hormone ( α ‐MSH) pAb (Sera‐lab, Sussex, UK) (1:1000); goat anti‐human interleukin (IL)‐1 α pAb (British Biotechnology, Oxon, UK) (1:1000); mouse anti‐human IL‐2 mAb (Serva, Heidelberg, Germany) (1:100); mouse anti‐human IL‐6 mAb (Serva) (1:100); goat anti‐human tumour necrosis factor (TNF)‐ α pAb (British Biotechnology) (1:1000); murine anti‐cytokeratin AE3 mAb (Cambridge Research Lab., MA, USA) (1:50); mouse anti‐cytokeratin AE1 mAb (Cambridge Research Lab.) (1:100); mouse anti‐vimentin mAb (BioMakor, Rehovot, Israel) (1:200).…”

Dendritic cells in the bursal follicles and germinal centers of the chicken's caecal tonsil express vimentin but not desmin

“…The bursal secretory dendritic cell of the bursa medulla expressed vimentin (Olah and Glick, 1992a,b) but not desmin in the medulla. The ERC of the bursal medullary region expressed keratin but not vimentin (Olah et al, 1992) and desmin. In the GC, anti-vimentin identified large positive cells similar to the bursal secretory dendritic cell while the anti-desmin recognized a loose network.…”