Differentiation of Human Skeletal Muscle Stem Cells into Odontoblasts Is Dependent on Induction of α1 Integrin Expression

Ozeki, Nobuaki; Mogi, Makio; Yamaguchi, Hideyuki; Hiyama, Taiki; Kawai, Rie; Hase, Naoko; Nakata, Kohei; Nakamura, Hiroshi; Kramer, Randall H.

doi:10.1074/jbc.m113.526772

Cited by 23 publications

(14 citation statements)

References 55 publications

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“…Recently, we established conditions for efficient conversion of human muscle stem cells to an odontoblast lineage (48). We will use these cells to revisit our previous and current studies on rat fibroblast-like cells to determine the extent to which our findings are relevant in the human system.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous study, a cytokine cocktail induced expression of MMP-3 that regulated cell proliferation and suppressed apoptosis in mouse ES cell-derived odontoblast-like cells (47) and human skeletal muscle stem cell-derived odontoblast-like cells (48). Interleukin-1β and the cytokine cocktail also induced MMP-3-regulated cell proliferation and suppressed apoptosis in partially purified rat dental pulp cells (18,19).…”

Section: Discussionmentioning

confidence: 99%

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Retraction: Polyphosphate-induced matrix metalloproteinase-3-mediated proliferation in rat dental pulp fibroblast-like cells is mediated by a Wnt5 signaling cascade

Ozeki

Yamaguchi

Hase

et al. 2015

BST

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Retraction: Polyphosphate-induced matrix metalloproteinase-3-mediated proliferation in rat dental pulp fibroblast-like cells is mediated by a Wnt5 signaling cascade

Ozeki

Yamaguchi

Hase

et al. 2015

BST

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“…Previously, we established a proliferating dental pulp progenitor cell line, KN-3, from dental papilla cells of rat incisors 15) . We reported that KN-3 cells express high levels of alkaline phosphatase (ALP) activity, as well as runtrelated transcription factor 2 and dentin sialophosphop rotein [15][16][17] . In addition, we showed that KN-3 cells form mineralized nodules by treatment with ascorbic acid and β-glycerophosphate in an in vitro culture system.…”

Section: Ifn-γ Is An Important Proinflammatory Cytokine Thatmentioning

confidence: 99%

Effects of Interferon-γ on odontoblastic differentiation and mineralization of odontoblast-like cells

Nakagawa

Okinaga

Ariyoshi

et al. 2015

Inflammation and Regeneration

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Introduction: Dentinogenesis is regulated by cytokines and growth factors, and modulated by alterations in the extracellular microenvironment. Dental matrix protein-1 (DMP-1), which is predominantly expressed in odontoblasts, is required during the early and late stages of odontogenesis. In the present study, we examined the involvement of proinflammatory cytokines in the expression of odontogenic markers in the rat odontoblast-like cell line KN-3. Methods: The expression of DMP-1 and p38 mitogen-activated protein kinase (MAPK) in proinflammatory cytokine-treated KN-3 cells was evaluated by immunoblot analysis. Alkaline phosphatase (ALP) activity in proinflammatory cytokine-treated KN-3 cells was measured by ALP staining and a colorimetric assay. Results: DMP-1 protein was downregulated in KN-3 cells treated with interferon-γ (IFN-γ) for 3 days, but not in interleukin-1β (IL-1β)-treated cells. The IFN-γ-induced downregulation of DMP-1 was rescued by treatment with IL-1β for 3 days. Interestingly, ALP activity was also suppressed in IFN-γtreated KN-3 cells and no significant change was induced by IL-1β treatment for 5 days. In addition, IFN-γ treatment for 3 days remarkably upregulated the phosphorylation of p38 MAPK in KN-3 cells. Conclusions: INF-γ treatment downregulated DMP-1 expression and ALP activity in KN-3 cells through prolonged phosphorylated p38 MAPK. IL-1β treatment restored these odontogenic reactions mediated by IFN-γ. Taken together, these findings suggest that IFN-γ and IL-β may be involved in the complex regulation of odontogenesis in the microenvironment of dental pulp. Rec.3/18/2015, Acc.5/24/2015, pp210-217

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“…The localization and activation of APase in odontoblasts and pulpal fibroblasts or progenitor cells point to the role of APase in cell differentiation during dentine formation. Stem cell-based tooth regeneration is a promising approach to solving the problems of dentine caries and human skeletal muscle stem cells could also differentiate into odontoblasts but required an extracellular matrix scaffold and changes in their integrin profile that was evidenced by appearance of odontoblastic phenotypes [ 53 ]. Therefore, cellular responses of pulpal origin against dental caries seem evident.…”

Section: Discussionmentioning

confidence: 99%

Enzymes, Dentinogenesis and Dental Caries: a Literature Review

Larmas¹,

Sándor

2014

JOMR

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ObjectivesSearch in PubMed with keywords “enzymes, dentinogenesis, and dental caries” revealed only 4 items, but when combined with “enzymes, osteogenesis, and osteoporosis” as high as 404 items resulted. Dental caries was associated with an order of magnitude fewer studies than the chronic bone disease, osteoporosis. This observation motivated this review.Material and MethodsA comprehensive review of the available literature on role of enzymes in dentinogenesis and dental caries was undertaken using MEDLINE (PubMed) and Scopus. Keywords for the search were: enzymes and odontoblasts, enzymes and different forms of dentinogenesis as well as dental caries.ResultsSearch revealed studies which described odontoblasts harbouring numerous enzymes (hydrolases, including metalloproteinases, transaminases and dehydrogenases) during primary dentinogenesis. Alkaline phosphatase activity sharply decreased when odontoblasts turned into quiescent odontoblasts. Tertiary dentinogenesis was characterized first by reactionary dentine formation when alkaline phosphatase was highly reactivated. Then later some of these odontoblasts may die out and be replaced by other progenitor cells of pulpal origin. This tertiary dentine was called reparative dentine. Pulpal progenitor/stem cells revealed alkaline phosphatase activity in areas encircling inflamed pulp sections. Soft carious dentine revealed high hydrolase, transaminase and dehyrogenase activities that may have originated from invading microbes, saliva or were endogenous. Proteolytic activity was especially demonstrable using histochemical and biochemical means. Specifically, matrix metalloproteases may have originated partly from activated proenzymes of host origin.ConclusionsThough dental studies are scanty when compared to bone, the active role of large spectrum of enzymes in healthy and carious dentine was given support.

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Differentiation of Human Skeletal Muscle Stem Cells into Odontoblasts Is Dependent on Induction of α1 Integrin Expression

Cited by 23 publications

References 55 publications

Retraction: Polyphosphate-induced matrix metalloproteinase-3-mediated proliferation in rat dental pulp fibroblast-like cells is mediated by a Wnt5 signaling cascade

Retraction: Polyphosphate-induced matrix metalloproteinase-3-mediated proliferation in rat dental pulp fibroblast-like cells is mediated by a Wnt5 signaling cascade

Effects of Interferon-γ on odontoblastic differentiation and mineralization of odontoblast-like cells

Enzymes, Dentinogenesis and Dental Caries: a Literature Review

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