Differentiation of human umbilical cord blood CD133+ stem cells towards myelo–monocytic lineage

Ruzicka, Katharina; Gršković, Branka; Pavlović, Vladimir; Qujeq, Durdi; Karimi, Alireza; Mueller, Mathias M.

doi:10.1016/j.cccn.2003.11.019

Cited by 21 publications

(21 citation statements)

References 25 publications

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“…In our study, in the presence of cytokines, CD133 + cells co-expressing CD13 and CD36 increased from 26% at D0 to 72% after culture for 14 days in EPO (E14), but decreased to 13% after culture for 14 days in G-CSF (G14). Meanwhile, in both E14 and G14 cultures, CD133 + CD13 − CD36 − or CD34 + CD13 − CD36 − cells decreased to less than 1% from their original >97% at D0, which is consistent with a previous report [28]. It is unlikely that the minimal number of CD133 + CD13 − CD36 − or CD34 + CD13 − CD36 − cells at D14 can give rise to abundant erythroid or myeloid lineage cells at this point [14].…”

Section: Discussionsupporting

confidence: 91%

Identification of CD13+CD36+ cells as a common progenitor for erythroid and myeloid lineages in human bone marrow

Chen

Gao

Zhu

et al. 2007

Experimental Hematology

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Objective-To identify bi-potential precursor cells of erythroid and myeloid development in human bone marrow.Materials and Methods-Cells co-expressing CD13 and CD36 (CD13 + CD36 + ) were investigated by analyzing cell surface marker expression during erythroid development (induced with a combination of cytokines plus erythropoietin [EPO]), or myeloid development (induced with the same cocktail of cytokines plus granulocyte-colony stimulating factor [G-CSF]) of bone marrow derived CD133 cells in liquid cultures. CD13 + CD36 + subsets were also isolated on the 14 th day of cultures and further evaluated for their hematopoietic clonogenic capacity in methylcellulose.Results-Colony-forming analysis of sorted CD13 + CD36 + cells of committed erythroid and myeloid lineages demonstrated that these cells were able to generate erythroid, granulocyte, and mixed erythroid -granulocyte colonies. In contrast, CD13 + CD36 − or CD13 − CD36 + cells exclusively committed to granulocyte/monocyte or erythroid colonies, respectively, but failed to form mixed erythroid -granulocyte colonies; no colonies were detected in CD13 − CD36 − cells with lineagesupporting cytokines. In addition, our data confirmed that EPO induced both erythroid and myeloid commitment, while G-CSF only supported the differentiation of the myeloid lineage.Conclusions-The present data identify some CD13 + CD36 + cells as bi-potential precursors of erythroid and myeloid commitment in normal hematopoiesis. They provide a physiological explanation for the cell identification of myeloid and erythroid lineages observed in hematopoietic diseases. This unique fraction of CD13 + CD36 + cells may be useful for further studies on regulating erythroid and myeloid differentiation during normal and malignant hematopoiesis.

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Section: Discussionsupporting

confidence: 91%

Identification of CD13+CD36+ cells as a common progenitor for erythroid and myeloid lineages in human bone marrow

Chen

Gao

Zhu

et al. 2007

Experimental Hematology

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“…In contrast, an increase in cells expressing CD36 (from 1.1% to 87.7%; Figure 4, Ca, Cb) or CD13 (from 46.3% to 98.6%; Figure 4, Ca, Ce) was observed in 14-day cultures with EPO (E14) and G-CSF (G14), respectively. In E14 cells, 76.6% of CD36-expressing cells co-expressed the CD13 antigen (Figure 4, Cb), whereas in G14 cells, only 6.1% of CD13-expressing cells co-expressed the CD36 marker (Figure 4 (Figure 4, Ab, Bb) or G14 ( Figure 4, Ae, Be) cell populations, consistent with a previously published study [9]. Five to six percent of CD133-expressing cells co-expressed the CD13 antigen (Figure 4, Ab) or the CD36 antigen (Figure 4, Bb) in E14 cells, whereas in G14 cells, <1% of CD133-expressing cells co-expressed CD13 (Figure 4, Ae) or CD36 (Figure 4, Be).…”

Section: Co-expression Of Cell Surface Marker In Cytokine-mediated LIsupporting

confidence: 91%

“…In this study, we used CD133 (also known as AC133)-selected, highly enriched HSCs, which have been used to study stem/progenitor cell biology as an alternative to using CD34-selected cells [8][9][10]. For lineage-specific markers, we used CD13 as a myeloid antigen that is highly expressed on early myeloblast precursors and promyelocytes [11,12] and CD36 as an erythroid marker of proerythroblasts and basophilic erythroblasts of early erythroid precursor cells [13].…”

Section: Introductionmentioning

confidence: 99%

Identification of key genes responsible for cytokine-induced erythroid and myeloid differentiation and switching of hematopoietic stem cells by RAGE

Chen

Zhang²,

Shi

et al. 2006

Cell Res

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We utilized a unique culture system to analyze the expression patterns of gene, protein, and cell surface antigen, and the biological process of the related genes in erythroid and myeloid differentiation and switching of hematopoietic stem cells (HSCs) in response to cytokine alterations. Gene-specific fragments (266) identified from five populations of cytokine-stimulated HSCs were categorized into three groups: (1) expressed specifically in a single cell population; (2) expressed in two cell populations, and (3) expressed in three or more populations. Of 145 defined cDNAs, three (2%) were novel genes. Protein two-dimensional gel electrophoresis and flow cytometry analyses showed overlapped and distinguished protein expression profiles in the cell populations studied. Biological process mapping of mRNAs expressed in erythroid and myeloid lineages indicated that mRNAs shared by both lineages attended 'core processes,' whereas genes specifically expressed in either lineage alone were related to specific processes or cellular maturation. Data from this study support the hypothesis that committed HSCs (E14 or G14) cells can still be redirected to develop into myeloid or erythroid cells when erythropoietin (EPO) is replaced with granulocyte-colony stimulating factor (G-CSF) under erythroid-cultured condition or G-CSF with EPO in myeloid-cultured environment, respectively. Our results suggest that genes or proteins co-expressed in erythroid and myeloid lineages may be essential for the lineage maintenance and switching in hematopoiesis.Cell Research (2006) 16:923-939.

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“…However, a significant increase of CD14 expressing monocyte/macrophages was observed in the co-culture of X-irradiated cells in comparison to the stroma-free culture. These results may be due to the large increase of CFU-GM, monocyte/macrophage progenitors, in the culture (Ruzicka et al 2004;Stec et al 2007).…”

Section: Discussionmentioning

confidence: 82%

Placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells support hematopoietic recovery of X-irradiated human CD34+ cells

Hayashi¹,

Takahashi²,

Abe³

et al. 2009

Life Sciences

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Differentiation of human umbilical cord blood CD133+ stem cells towards myelo–monocytic lineage

Cited by 21 publications

References 25 publications

Identification of CD13+CD36+ cells as a common progenitor for erythroid and myeloid lineages in human bone marrow

Identification of CD13+CD36+ cells as a common progenitor for erythroid and myeloid lineages in human bone marrow

Identification of key genes responsible for cytokine-induced erythroid and myeloid differentiation and switching of hematopoietic stem cells by RAGE

Placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells support hematopoietic recovery of X-irradiated human CD34+ cells

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