Differentiation of MC3T3-E1 on poly(4-styrenesulfonic acid-co-maleic acid)sodium salt-coated films

Angwarawong, Thidarat; Dubas, Stephan Thierry; Arksornnukit, Mansuang; Pavasant, Prasit

doi:10.4012/dmj.2010-097

Cited by 16 publications

(16 citation statements)

References 62 publications

(77 reference statements)

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“…One major requirement of any tissue engineering scaffold is biocompatibility, which is the ability to support normal cellular activity without any toxic effects to the host tissue (Williams, 2008). The biocompatibility of DTS and CETC were evaluated using MC3T3-E1 cells, an immortalized murine preosteogenic cell-line commonly used to assess in vitro osteogenic development (Angwarawong, Dubas, Arksornnukit, & Pavasant, 2011). When seeded on DTS and CETC, MC3T3-E1 cells attached and proliferated well, as shown by the increasing metabolic activity over time in the Alamar blue assay (Figure 3b) as well as the spreading of cells in SEM ( Figure 3a).…”

Section: In Vitro Characterization Of Dts and Cetcmentioning

confidence: 99%

“…In osteogenic media, MC3T3-E1 cells would undergo osteogenic differentiation, which involves the phases of proliferation, maturation, and eventually mineralization (Owen et al, 1990). These phases could be identified by the upregulation of osteogenic genes, the increased production of osteogenic proteins, and the production of mineralized matrix (Angwarawong et al, 2011). Among the various osteogenic genes, BSP, OC, and OPN were used as markers to investigate osteogenic differentiation in this study.…”

Section: In Vitro Characterization Of Dts and Cetcmentioning

confidence: 99%

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Evaluation of decellularized tilapia skin as a tissue engineering scaffold

Lau

Hassanbhai

Wen

et al. 2019

J Tissue Eng Regen Med

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Decellularized bovine and porcine tissues have been used as scaffolds to support tissue regeneration but inherit religious restrictions and risks of disease transmission to humans. Decellularized marine tissues are seen as attractive alternatives due to their similarity to mammalian tissues, reduced biological risks, and less religious restrictions. The aim of this study was to derive an acellular scaffold from the skin of tilapia and evaluate its suitability as a tissue engineering scaffold. Tilapia skin was treated with a series of chemical and enzymatic treatments to remove cellular materials. The decellularized tilapia skin (DTS) was then characterized and evaluated in vitro and in vivo to assess its biological compatibility. The results indicated that the decellularization process removed 99.6% of the DNA content from tilapia skin. The resultant DTS was shown to possess a high denaturation temperature of 68.1 ± 1.0°C and a high Young's modulus of 56.2 ± 14.4 MPa. The properties of DTS were also compared against those of crosslinked electrospun tilapia collagen membrane, another form of tilapia‐derived collagen scaffold. In vitro studies revealed that both DTS and crosslinked electrospun tilapia collagen promoted cellular metabolic activity, differentiation, and mineralization of murine preosteogenic MC3T3‐E1 cells. The rat calvarial defect model was used to evaluate the in vivo performance of the scaffolds, and both scaffolds did not induce hyperacute rejections. Furthermore, they enhanced bone regeneration in the critical defect compared with the sham control. This study suggests that tilapia‐derived scaffolds have great potential in tissue engineering applications.

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Section: In Vitro Characterization Of Dts and Cetcmentioning

confidence: 99%

Section: In Vitro Characterization Of Dts and Cetcmentioning

confidence: 99%

Evaluation of decellularized tilapia skin as a tissue engineering scaffold

Lau

Hassanbhai

Wen

et al. 2019

J Tissue Eng Regen Med

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“…Poly(NaSS) plays an important role in the production of this enzyme [38] and that effect was reflected in all tested conditions. Its influence was the most significant for the combination Col I ?…”

Section: Differentiation and Mineralizationmentioning

confidence: 89%

Contributions of adhesive proteins to the cellular and bacterial response to surfaces treated with bioactive polymers: case of poly(sodium styrene sulfonate) grafted titanium surfaces

Felgueiras

Aissa

Evans

et al. 2015

J Mater Sci: Mater Med

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The research developed on functionalized model or prosthetic surfaces with bioactive polymers has raised the possibility to modulate and/or control the biological in vitro and in vivo responses to synthetic biomaterials. The mechanisms underlying the bioactivity exhibited by sulfonated groups on surfaces involves both selective adsorption and conformational changes of adsorbed proteins. Indeed, surfaces functionalized by grafting poly(sodium styrene sulfonate) [poly(NaSS)] modulate the cellular and bacterial response by inducing specific interactions with fibronectin (Fn). Once implanted, a biomaterial surface is exposed to a milieu of many proteins that compete for the surface which dictates the subsequent biological response. Once understood, this can be controlled by dictating exposure of active binding sites. In this in vitro study, we report the influence of binary mixtures of proteins [albumin (BSA), Fn and collagen type I (Col I)] adsorbed on poly(NaSS) grafted Ti6Al4V on the adhesion and differentiation of MC3T3-E1 osteoblast-like cells and the adhesion and proliferation of Staphylococcus aureus (S. aureus). Outcomes showed that poly(NaSS) stimulated cell spreading, attachment strength, differentiation and mineralization, whatever the nature of protein provided at the interface compared with ungrafted Ti6Al4V (control). While in competition, Fn and Col I were capable of prevailing over BSA. Fn played an important role in the early interactions of the cells with the surface, while Col I was responsible for increased alkaline phosphatase, calcium and phosphate productions associated with differentiation. Poly(NaSS) grafted surfaces decreased the adhesion of S. aureus and the presence of Fn on these chemically altered surfaces increased bacterial resistance ≈70% compared to the ungrafted Ti6Al4V. Overall, our study showed that poly(NaSS) grafted Ti6Al4V selectively adsorbed proteins (particularly Fn) promoting the adhesion and differentiation of osteoblast-like cells while reducing bacterial adhesion to create a bioactive surface with potential for orthopaedic applications.

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“…[38,39] In our study, we synthesized a novel biologically PTH-related peptide, PTHrP-1, and examined its dose-dependence on the osteogenic induction of MC3T3-E1 cells in vitro, which could improve the proliferation and differentiation of MC3T3-E1 cells and maintained their morphology. However, Zhang et al [40] reported that 3 different cell models were utilized for differentiation studies in vitro in order to determine whether there was variability among cell lines or there were cell type-specific effects.…”

Section: Discussionmentioning

confidence: 99%

Dose-dependence of PTH-related peptide-1 on the osteogenic induction of MC3T3-E1 cells in vitro

Wang

Yang

et al. 2017

Medicine

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Parathyroid hormone (PTH), an 84-amino acid peptide, is an endocrine hormone that is secreted by parathyroid glands. PTH performs important functions in calcium regulation and bone remodeling. The PTH (1–34) named teriparatide, a 34-amino acid peptide derived from the N-terminus of PTH, conserves most of the functions of PTH, specifically the osteogenic capability. However, teriparatide is only used by injection and exhibits short duration. In addition, this PTH could not thoroughly expose active sites. In this study, a novel PTH-related peptide (designated PTHrP-1) derived from the N-terminus of PTH was added into the complete medium at different concentrations of PTHrP-1 (0, 50, 100, and 200 ng/mL) to induce the MC3T3-E1 cells. PTHrP-1 was detected by high-performance liquid chromatography and matrix-assisted laser desorption/ionization–time-of-flight mass spectroscopy. Cell morphology, cell proliferation, alkaline phosphatase (ALP), and ALP activity, osteocalcin concentration, and collagen type I (Col-I), osteopontin (OPN), and osteocalcin (OCN) mRNA expression by RT-PCR and protein expression by western blotting were observed and detected. The purity of the PTHrP-1 was 95.14%, and the PTHrP-1 can induce MC3T3-E1 cells into osteoblasts, thus improving ALP activity and OCN concentration, and increasing Col-I, OPN, and OCN mRNA expression and protein expression in MC3T3-E1 cell cultures. The PTHrP-1 proved to be an ideal active peptide. In addition, the osteogenic ability of PTHrP-1 at 200 and 100 ng/mL concentrations was not significantly different but significantly higher than 50 and 0 ng/mL groups. Results indicate that PTHrP-1 is a kind of active peptides that exhibits good biocompatibility with MC3T3-E1 cells and could improve cell proliferation and osteogenic differentiation. Moreover, PTHrP-1, at the preferable concentration of 100 ng/mL, could effectively promote MC3T3-E1 cells into osteoblasts.

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Differentiation of MC3T3-E1 on poly(4-styrenesulfonic acid-co-maleic acid)sodium salt-coated films

Cited by 16 publications

References 62 publications

Evaluation of decellularized tilapia skin as a tissue engineering scaffold

Evaluation of decellularized tilapia skin as a tissue engineering scaffold

Contributions of adhesive proteins to the cellular and bacterial response to surfaces treated with bioactive polymers: case of poly(sodium styrene sulfonate) grafted titanium surfaces

Dose-dependence of PTH-related peptide-1 on the osteogenic induction of MC3T3-E1 cells in vitro

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