Differentiation of Neisseria gonorrhoeae isolates requiring proline, citrulline, and uracil by plasmid content, serotyping, and pulsed-field gel electrophoresis

Ng, L.-K.; Carballo, M; Dillon, J A

doi:10.1128/jcm.33.4.1039-1041.1995

Cited by 24 publications

(10 citation statements)

References 13 publications

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“…According to these results PFGE fingerprinting is a sharper and more discriminating epidemiologic tool than the routinely used serological typing. This is in full agreement with previous studies [82,83,87].…”

Section: Typing Methodssupporting

confidence: 94%

“…From each suspension 300 µL was used for preparation and digestion of the genomic DNA of the bacteria according to the manufacturer's protocol of GenePath Group 3 Reagent Kit (Bio-Rad Laboratories, Hercules, CA, USA). Two different restriction enzymes were separately used: SpeI recommended by Bio-Rad and used in previous studies [82][83][84][85][86] and BglII also used in previous studies [86,87].…”

Section: Genetic Characterisation By Pfgementioning

confidence: 99%

See 1 more Smart Citation

The Epidemiology of Gonorrhea Among Men Who Have Sex With Men in Stockholm, Sweden, 1990–2004

Berglund

Asikainen²,

Grützmeier³

et al. 2007

Sexually Transmitted Diseases

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Section: Typing Methodssupporting

confidence: 94%

Section: Genetic Characterisation By Pfgementioning

confidence: 99%

The Epidemiology of Gonorrhea Among Men Who Have Sex With Men in Stockholm, Sweden, 1990–2004

Berglund

Asikainen²,

Grützmeier³

et al. 2007

Sexually Transmitted Diseases

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show abstract

“…Chromosomal DNA was prepared by the procedure described by Van Looveren et al (27), except that no lysis step was performed. Four restriction enzymes used in other studies for the typing of gonococci, BglII, NheI, SpeI, and XbaI (13,14,19,22,32), were tested under various running conditions. All produced comparable results.…”

Section: Ardramentioning

confidence: 99%

“…Isoenzyme typing based on multilocus enzyme electrophoresis is widely applicable for epidemiological studies of diverse groups of pathogens, including N. gonorrhoeae (16,18,23). Genetic relatedness of gonococcal isolates has also been assessed by using DNA-based typing techniques, including restriction endonuclease analysis using frequently or rarely cut-ting enzymes (7,14,20,31,32), and random and repetitivemotif-based amplification of polymorphic DNA fragments (2,21). The discriminatory abilities of pulsed-field gel electrophoresis (PFGE) and random and repetitive-motif-based amplification of polymorphic DNA have been shown to be superior to that of traditional A/S typing (2,14,21,32).…”

mentioning

confidence: 99%

Evaluation of the Discriminatory Power of Typing Methods forNeisseria gonorrhoeae

et al. 1999

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A panel of 18 strains of Neisseria gonorrhoeae, known to be temporally and geographically diverse, was used to evaluate a number of typing systems, including conventional auxotyping and serotyping and the molecular methods of arbitrarily primed PCR (AP-PCR), amplified ribosomal-DNA restriction analysis (ARDRA), opa typing, and pulsed-field gel electrophoresis (PFGE). The discriminatory power of the different typing methods were determined with a collection of 87 clinical isolates from commercial sex workers in Indonesia, and Simpson's index of diversity was calculated. Of the two traditional techniques, auxotyping and serotyping, the latter gives the highest discriminatory index (DI) (DI, 0.846). The combination of auxotyping and serotyping yields a high DI (DI, 0.928). D11344-and D8635-primed PCR showed low DIs of 0.608 and 0.622, respectively, but a combination of the two primers had a DI of 0.849. The combination of serotyping with D11344-primed or D8635-primed PCR resulted in DIs of 0.936 and 0.937, respectively. ARDRA revealed a low DI of 0.743 alone but a DI of 0.955 in combination with serotyping. PFGE using the restriction enzyme BglII and opa typing produced the highest discrimination (DIs, 0.997 and 0.996, respectively) for isolates of N. gonorrhoeae.

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“…However, a short or long transmission chain has not been defined by the numbers of sexual partners. It has been shown that the majority of isolates of N. gonorrhoeae from sexual contacts have the same phenotype (3,11) and genotype, determined by a variety of genotypic methods (10,17,22,24,25). As N. gonorrhoeae is highly efficient at all points of its life cycle for recombination (2), persistence of a particular genotype over time should not in theory occur, particularly in genes where genetic variations accumulate very rapidly.…”

mentioning

confidence: 99%

Persistence of Two Genotypes of Neisseria gonorrhoeae during Transmission

Martin¹,

Ghani

Bell

et al. 2003

J Clin Microbiol

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Isolates of Neisseria gonorrhoeae were tested using a highly discriminatory typing method, opa typing, to examine the genetic diversity over a 2-year study period of isolates from all consecutive patients with gonorrhea attending the Genitourinary Medicine clinic in Sheffield, United Kingdom. Two opa genotypes were detected throughout the 2-year time period and comprised 41% of all strains tested. The persistence of two opa types was investigated further to determine the apparent genetic stability, by examining the ability of isolates to undergo intragenic and intergenic recombination and mutation in vitro. Intragenic recombination or mutation involving the opa genes of N. gonorrhoeae in the selected isolates was not detected, but intergenic recombination did occur. opa genes of N. gonorrhoeae in vivo appear to diversify primarily through intergenic recombination. Intergenic recombination in vivo would require the presence of a mixed gonococcal infection, in which an individual is concurrently colonized with more than one strain of N. gonorrhoeae. We propose that the level of diversity of opa genotypes in a population is linked to the degree of sexual mixing of individuals and the incidence of mixed infections of N. gonorrhoeae.

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Differentiation of Neisseria gonorrhoeae isolates requiring proline, citrulline, and uracil by plasmid content, serotyping, and pulsed-field gel electrophoresis

Cited by 24 publications

References 13 publications

The Epidemiology of Gonorrhea Among Men Who Have Sex With Men in Stockholm, Sweden, 1990–2004

The Epidemiology of Gonorrhea Among Men Who Have Sex With Men in Stockholm, Sweden, 1990–2004

Evaluation of the Discriminatory Power of Typing Methods forNeisseria gonorrhoeae

Persistence of Two Genotypes of Neisseria gonorrhoeae during Transmission

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