Differentiation of rabbit adipocyte precursors in primary culture

Newly developed primary culture of rat visceral adipocytes and their in vitro characteristics

“…Chylomicron collected from the mesenteric lymph duct stimulated enzymatic activity for fat metabolism of a primary adipocyte culture in rabbits (Nougues et al, 1988). These fats added in the medium were absorbed in cells and accumulated in the lipid particles in the cytoplasm.…”

“…In studies of the physiological regulatory mechanism, including cell differentiation and proliferation, a primary cell culture system has an advantage over established cell lines. For intra-abdominal adipose tissues, preliminary experiments have been performed using fat tissue derived from rats (Van and Roncari, 1978;Bjorntorp et al, 1980), rabbits (Nougues et al, 1988), dogs (Wu et al, 2001), pigs (Hausman et al, 1984), sheep (Broad and Ham, 1983), cattle (Cryer et al, 1984;Wu et al, 2000) and humans (Van and Roncari, 1978;Wabitsch et al, 2001). Although these experiments have provided useful data, a reproducible and reliable culture system, allowing a constant supply of preadipogenic cells isolated from experimental animals, still needs to be established.…”

“…Although these experiments have provided useful data, a reproducible and reliable culture system, allowing a constant supply of preadipogenic cells isolated from experimental animals, still needs to be established. With regard to anatomical depots, epididymal or perirenal adipose tissue, but not mesenteric adipose tissue, were used in previous reports concerned with intra-abdominal preadipocytes in rats ( Van and Roncari, 1978;Bjorntorp et al, 1980) and rabbits (Nougues et al, 1988). Thus, it is difficult to regard them as a reliable and established culture system for VCs using mesenteric adipose tissue isolated from experimental animals.…”

Pathophysiological dilemmas of lipedema

“…According to Brorson et al, liposuction can be an effective treatment for patients with fat accumulation due to lymphedema [62]. Nougues and colleagues studying rabbit adipocytes observed enhanced differentiation and lipid accumulation caused by mesenteric lymph and chylomicrons in the culture medium [63]. Further animal studies indicate that lymphangiogenetic defects cause increased fat deposition [21,22].…”

Current views on the function of the lymphatic vasculature in health and disease

“…vessels and adipose metabolism. Additionally, ectopic adipose tissue growth is observed in edematous regions of patients suffering from chronic lymphedema (Brorson 2003), and lymph or chyle (i.e., a mixture of lymph and chylomicrons with a milky appearance) promotes the differentiation of rabbit preadipocytes in vitro (Nougues et al 1988). Furthermore, a correlation between immune cells, lymph nodes, and their surrounding adipose tissue has been documented (Pond andMattacks 1995, 2003;Pond 2003).…”