Diffusion-weighted MRI and 18F-FDG PET correlation with immunity in early radiotherapy response in BNL hepatocellular carcinoma mouse model: timeline validation

Chung, Y.; Yu, Chih-Teng; Chiu, Shao-Chieh; Chiu, Hsien Chin; Hsu, Shin-Ting; Wu, Chia-Huang; Yang, Chien‐Hsin; Hong, Ji-Hong; Yen, Tzu‐Chen; Chen, Fang-Hsin

doi:10.1007/s00259-019-04318-3

Cited by 11 publications

(21 citation statements)

References 34 publications

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“…We have previously shown that local irradiation induces a continuous influx of macrophages at sites in orthotopic hepatic neoplasms [ 16 ], which was in turn, associated with a higher tumor 18F-FDG uptake. Herein, we have shown that 18F-FDG uptake is increased in normal hepatic tissue on post-RT day 3.…”

Section: Resultsmentioning

confidence: 99%

“…In the previous report, the increased FDG uptake in HCC tumor lesion was found in post-RT day one to day six due to macrophages infiltration [ 16 ]. In this study, we observed that the FDG uptake of the irradiated liver parenchyma was significantly higher than that of non-irradiated group in post-RT day three.…”

Section: Discussionmentioning

confidence: 99%

“…Ethical approval for all animal experiments was received from the Institutional Animal Care and Use Committee of the National Tsing Hua University (approval number: 10414) and the Chang Gung Memorial Hospital (approval number: 2016010701). The animal model and the procedures used for irradiation have been previously described in detail [16].…”

Section: Animal Model and Procedures For Irradiationmentioning

confidence: 99%

“…The radiation dose 15 Gy is a clinically relevant daily dose that achieves over 50% tumor control rate without normal tissue toxicity [39]. Therefore, the right liver was partially irradiated with 20 × 10 mm collimated irradiation field and 6-MV X-ray beams (15 Gy) obtained from a clinical linear accelerator and covered with a 0.5-cm bolus to the animals' skin [16]. 1 The right lobe of each mouse in the RT group was received 15 Gy irradiation.…”

Section: Animal Model and Procedures For Irradiationmentioning

confidence: 99%

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Radiation-Induced Metabolic Shifts in the Hepatic Parenchyma: Findings from 18F-FDG PET Imaging and Tissue NMR Metabolomics in a Mouse Model for Hepatocellular Carcinoma

Chung

Tsai

et al. 2021

Molecules

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Purpose: By taking advantage of 18F-FDG PET imaging and tissue nuclear magnetic resonance (NMR) metabolomics, we examined the dynamic metabolic alterations induced by liver irradiation in a mouse model for hepatocellular carcinoma (HCC). Methods: After orthotopic implantation with the mouse liver cancer BNL cells in the right hepatic lobe, animals were divided into two experimental groups. The first received irradiation (RT) at 15 Gy, while the second (no-RT) did not. Intergroup comparisons over time were performed, in terms of 18F-FDG PET findings, NMR metabolomics results, and the expression of genes involved in inflammation and glucose metabolism. Results: As of day one post-irradiation, mice in the RT group showed an increased 18F-FDG uptake in the right liver parenchyma compared with the no-RT group. However, the difference reached statistical significance only on the third post-irradiation day. NMR metabolomics revealed that glucose concentrations peaked on day one post-irradiation both, in the right and left lobes—the latter reflecting a bystander effect. Increased pyruvate and glutamate levels were also evident in the right liver on the third post-irradiation day. The expression levels of the glucose-6-phosphatase (G6PC) and fructose-1, 6-bisphosphatase 1 (FBP1) genes were down-regulated on the first and third post-irradiation days, respectively. Therefore, liver irradiation was associated with a metabolic shift from an impaired gluconeogenesis to an enhanced glycolysis from the first to the third post-irradiation day. Conclusion: Radiation-induced metabolic alterations in the liver parenchyma occur as early as the first post-irradiation day and show dynamic changes over time.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Animal Model and Procedures For Irradiationmentioning

confidence: 99%

Section: Animal Model and Procedures For Irradiationmentioning

confidence: 99%

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Radiation-Induced Metabolic Shifts in the Hepatic Parenchyma: Findings from 18F-FDG PET Imaging and Tissue NMR Metabolomics in a Mouse Model for Hepatocellular Carcinoma

Chung

Tsai

et al. 2021

Molecules

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“…Whether or not early radiation-induced influx of immune cells in the tumor microenvironment can be detected by MR imaging, e.g. as increased ADC values on DW MRI ( 86 ), and guide the optimal timing of immunotherapy, remains to be investigated.…”

Section: Mr-guided Radio-immunotherapy: Challenges and Opportunitiesmentioning

confidence: 99%

MR-Guided Radiotherapy: The Perfect Partner for Immunotherapy?

et al. 2021

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During the last years, preclinical and clinical studies have emerged supporting the rationale to integrate radiotherapy and immunotherapy. Radiotherapy may enhance the effects of immunotherapy by improving tumor antigen release, antigen presentation, and T-cell infiltration. Recently, magnetic resonance guided radiotherapy (MRgRT) has become clinically available. Compared to conventional radiotherapy techniques, MRgRT firstly allows for daily on-table treatment adaptation, which enables both dose escalation for increasing tumor response and superior sparing of radiosensitive organs-at-risk for reducing toxicity. The current review focuses on the potential of combining MR-guided adaptive radiotherapy with immunotherapy by providing an overview on the current status of MRgRT, latest developments in preclinical and clinical radio-immunotherapy, and the unique opportunities and challenges for MR-guided radio-immunotherapy. MRgRT might especially assist in answering open questions in radio-immunotherapy regarding optimal radiation dose, fractionation, timing of immunotherapy, appropriate irradiation volumes, and response prediction.

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Response Evaluation After Radiotherapy

Yang

Shen

et al. 2021

Radiotherapy of Liver Cancer

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Diffusion-weighted MRI and 18F-FDG PET correlation with immunity in early radiotherapy response in BNL hepatocellular carcinoma mouse model: timeline validation

Cited by 11 publications

References 34 publications

Radiation-Induced Metabolic Shifts in the Hepatic Parenchyma: Findings from 18F-FDG PET Imaging and Tissue NMR Metabolomics in a Mouse Model for Hepatocellular Carcinoma

Radiation-Induced Metabolic Shifts in the Hepatic Parenchyma: Findings from 18F-FDG PET Imaging and Tissue NMR Metabolomics in a Mouse Model for Hepatocellular Carcinoma

MR-Guided Radiotherapy: The Perfect Partner for Immunotherapy?

Response Evaluation After Radiotherapy

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