Digestibility of amino acids in pig diets containing Eurolysine bacterial protein or Pekilo protein, with special reference to a gas chromatographic method used in amino acid determination

Näsi, Matti; Huida, Lea

doi:10.23986/afsci.72107

Cited by 5 publications

(5 citation statements)

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“…The CP content of the feeds and in vitro residues were analysed as 6.25 * Kjeldahl N. Ether extract (EE) was analysed after acid hydrolysis. The amino acids of the barley samples and soya bean meal were determined by gas chromatography after hydrolysis with 6 N HCI at 110°C for 20 h (Näsi and Huida 1982). The barley samples were also analysed for neutral detergent fibre (NDF) content by the method of Robertson and Van Soest (1981), for total dietary fibre content by the method of Asp et al (1983) and for (3-glucans content by the method of McClear and GlennieHolmes (1985).…”

Section: Chemical Analysesmentioning

confidence: 99%

Effects of variety, soil type and nitrogen fertilizer supply on the nutritive value of barley for growing pigs

Valaja¹,

Suomi²,

Alaviuhkola³

et al. 1997

AFSci

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Section: Chemical Analysesmentioning

confidence: 99%

Effects of variety, soil type and nitrogen fertilizer supply on the nutritive value of barley for growing pigs

Valaja¹,

Suomi²,

Alaviuhkola³

et al. 1997

AFSci

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“…The recommended use of conventional distillers' feeds has been limited to 5-10% of diets in the earlier experiments (Alaviuhkola 1978, Suomi 1980, Newman and Gras 1983, but in some reports distillers dried grains with solubles have been successfully added up to 20% of the diet (Cromwell andStahly 1986,Cromwell andMonegue 1994). However, the high fibre content and decreased availability of protein, due to the severe drying process, are mainly responsible for the variable results with conventional distillers feeds (Näsi 1984, Cromwell et al 1993 There are some contradictions between the results of the performance experiments (Valaja et al 1995 and present study) and those of the digestibility and protein utilisation studies (Näsi and Aimonen 1992, Valaja and Näsi 1996, Buraczewska et al 1995) since the digstibility and protein utilisation ofWDS have been very high. So it is quite unlikely that low protein digestibility or utilisation of WDS could have caused the results of the present experiment.…”

Section: Discussionmentioning

confidence: 99%

“…The WDS was delivered weekly from the Alko Ltd. Koskenkorva plant during the experiment. It was the product of an integrated starchethanol process, which uses barley as a raw material (Näsi 1988). In this process, WDS was separated from wet distillers' solubles by centrifugation.…”

Section: Methodsmentioning

confidence: 99%

“…In the growth experiment with WDS, the performance of the pigs has been only satisfactory (Valaja et al 1995). However, the digestibility of amino acids and protein in WDS has been high in the former experiments (Näsi and Aimonen 1992, Valaja and Näsi 1996, Buraczewska et al 1996. Because of the conflicting results in the performance and digestibility experiments, more information was needed on the suitability of wet distillers' solids for growing-finishing pigs and the optimal lysine supplementation of the diet.…”

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confidence: 99%

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Lysine supplementation of barley wet distillers’ solids diets for growing-finishing pigs

Valaja¹,

Siljander-Rasi²,

Alaviuhkola³

et al. 1996

AFSci

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A growth experiment was conducted to study the effect of different lysine supplementation levels of barley wet distillers’ solids (WDS) diets on the performance and the carcass quality of pigs (25.5-95.5 kg live weight). A total of 160 pigs were divided into four groups of 40 animals. Four pigs of the same sex were placed in each pen and fed one of the four experimental diets; a barley-soyabean meal (SBM) control diet (167 g crude protein (CP) /kg dry matter (DM) and 7.6 g lysine/feed unit (FU)) or three similar barley-WDS diets (204 g CP/kg DM) which were supplemented with synthetic lysine to contain 7.7, 9.2 or 10.7 g lysine/FU. Lysine supplementation linearly improved the daily weight gain (DG) and feed conversion ratio (FCR) of the pigs during growing (1-5 weeks) (p < 0.001), finishing (6th week onward) (p < 0.001) and the whole experimental period (p < 0.001). However, during the whole growing-finishing period the performance of the pigs levelled off at the lysine level of 9.2 g/FU and daily supply of 18.0 g, respectively(quadratic effect: p < 0.05 for DG and p < 0.01 for FCR). The pigs on the SBM diet grew faster and more efficiently than did the pigs on the WDS diets with similar lysine content (p < 0.05).

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“…Gas-liquid chromatography has been used in assays for lysine (Soar and Buttery 1979), methionine (Bos et al 1983;Duncan et al 1984a,b) and for mixtures of AA (Moodie et al 1982;Nasi and Huida 1982). In a collaborative study, gas-liquid chromatography and ion exchange gave comparable values for lysine in fish meals (Barlow et al 1984).…”

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confidence: 99%

Estimation of Bioavailable Amino Acids in Feedingstuffs for Poultry and Pigs: A Review With Emphasis on Balance Experiments

Sibbald¹

1987

Can. J. Anim. Sci.

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A bioavailable nutrient has the potential to be utilized when ingested. Ideally, the nutrient requirements of animals and the nutrient concentrations of feedingstuffs should be expressed in terms of bioavailable units. Bioavailability is an abstract concept which cannot be measured but it can be estimated. The purpose of this review is to describe, evaluate and compare procedures used to estimate the concentrations of bioavailable amino acids (BAA) in feedingstuffs for pigs and poultry. Accurate and precise measurement of total amino acid (AA) concentrations is of fundamental importance to many assays. Problems are encountered both in making the AA available for measurement (hydrolysis) and in the measurement procedures. There is need for standardization of methodology but until this is achieved caution must be exercised when comparing data obtained by different techniques or even from different laboratories. In vitro assays for BAA are relatively fast and can be made without access to animals. Enzymic digestion has the potential to be a good estimator of BAA but is constrained by a lack of information about the bioavailabilities of the peptides which are liberated. Chemical assays and dye binding produce data which sometimes, but not always, correlate with biological estimates of BAA. The future of such assays seems to be in quality control where comparisons are made among samples within ingredients. Among the indirect biological assays, those based on microbial growth have received the most attention. The major weakness of such assays resides in the difference between animal and microbe in the ability to utilize peptides. Plasma free amino acids are not satisfactory estimators of BAA although they can help to identify limiting AA in feedingstuffs. Nitrogen digestion lacks the sensitivity to be a predictor of BAA. Growth assays are thought by many to be the ultimate measure of bioavailability but in fact they measure utility which is not the same. Nevertheless, growth assays can provide valuable estimates of BAA providing that their limitations are understood. The assays are expensive and slow and the results can be affected by many variables including changes in nutrient concentrations when a test material is added to an assay diet. Much of the review is devoted to balance experiments which require great care and attention to detail if reliable, reproducible data are to be obtained. Procedures have been refined during many years of use and with the advent of new equipment and techniques. Concern is currently focussed on corrections for metabolic and endogenous losses and on the effects of the microflora of the alimentary tract. Recent developments have increased assay efficiency, particularly with poultry. The balance experiment provides BAA estimates, that can be used effectively in diet formulation, at a much faster rate than growth assays. The final section of the review makes comparisons among various methods of estimating BAA. Interspecies comparisons are useful because they provide guidance when attempting to apply data derived with one species to formulate diets for another. The search for a single assay which provides data applicable to several species is a worthwhile objective. In the meantime, there are many assays used to estimate BAA which have specific applications and limitations. Key words: Pigs, poultry, amino acids, assay methodology, feedingstuffs, review

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Digestibility of amino acids in pig diets containing Eurolysine bacterial protein or Pekilo protein, with special reference to a gas chromatographic method used in amino acid determination

Cited by 5 publications

References 4 publications

Effects of variety, soil type and nitrogen fertilizer supply on the nutritive value of barley for growing pigs

Effects of variety, soil type and nitrogen fertilizer supply on the nutritive value of barley for growing pigs

Lysine supplementation of barley wet distillers’ solids diets for growing-finishing pigs

Estimation of Bioavailable Amino Acids in Feedingstuffs for Poultry and Pigs: A Review With Emphasis on Balance Experiments

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