Digital holographic microscopy for live cell applications and technical inspection

Kemper, Björn; Bally, Gert von

doi:10.1364/ao.47.000a52

Cited by 706 publications

(312 citation statements)

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“…QPI measures the path-length shifts associated with the dry mass of the specimen [4][5][6]67,90,91]. The elimination of the contrast agent removes one cause of sample variability as the sample is observed directly.…”

Section: Choice Of Cell Culture and Imaging Vesselmentioning

confidence: 99%

“…Using QPI, studies associated with both thickness and refractive index fluctuations can be performed [4]. Previous work has been reviewed by Kemper et al, who described new ways of monitoring the cellular morphology changes in response to drugs [5], and by Kim et al who presented a review of digital holography (DH), with emphasis on microscopy techniques and applications [6].…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Phase Imaging for Label-Free Analysis of Cancer Cells—Focus on Digital Holographic Microscopy

2018

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Abstract:To understand complex biological processes, scientists must gain insight into the function of individual living cells. In contrast to the imaging of fixed cells, where a single snapshot of the cell's life is retrieved, live-cell imaging allows investigation of the dynamic processes underlying the function and morphology of cells. Label-free imaging of living cells is advantageous since it is used without fluorescent probes and maintains an appropriate environment for cellular behavior, otherwise leading to phototoxicity and photo bleaching. Quantitative phase imaging (QPI) is an ideal method for studying live cell dynamics by providing data from noninvasive monitoring over arbitrary time scales. The effect of drugs on migration, proliferation, and apoptosis of cancer cells are emerging fields suitable for QPI analysis. In this review, we provide a current insight into QPI applied to cancer research.

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Section: Choice Of Cell Culture and Imaging Vesselmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative Phase Imaging for Label-Free Analysis of Cancer Cells—Focus on Digital Holographic Microscopy

2018

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“…It does not involve raster scanning. Most importantly, the phase image is a quantitative representation of the object profile with nanometer, and even subnanometer, precision [20,21,[46][47][48]. An example of DHM imaging of a layer of onion cells is shown in Fig.…”

Section: Quantitative Phase Microscopymentioning

confidence: 99%

Principles and techniques of digital holographic microscopy

2010

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Abstract. Digital holography is an emerging field of new paradigm in general imaging applications. We present a review of a subset of the research and development activities in digital holography, with emphasis on microscopy techniques and applications. First, the basic results from the general theory of holography, based on the scalar diffraction theory, are summarized, and a general description of the digital holographic microscopy process is given, including quantitative phase microscopy. Several numerical diffraction methods are described and compared, and a number of representative configurations used in digital holography are described, including off-axis Fresnel, Fourier, image plane, in-line, Gabor, and phase-shifting digital holographies. Then we survey numerical techniques that give rise to unique capabilities of digital holography, including suppression of dc and twin image terms, pixel resolution control, optical phase unwrapping, aberration compensation, and others. A survey is also given of representative application areas, including biomedical microscopy, particle field holography, micrometrology, and holographic tomography, as well as some of the special techniques, such as holography of total internal reflection, optical scanning holography, digital interference holography, and heterodyne holography. The review is intended for students and new researchers interested in developing new techniques and exploring new applications of digital holography. C 2010 Society of Photo-Optical Instrumentation Engineers.

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“…[1][2][3][4][5][6] This becomes particularly critical when the imaging technique is used for targeting cells for a chemical or radiation assault and subsequent long term tracking. Perhaps the most common method of cell visualization, fluorescent staining, particularly using UV-illumination, potentially induces cytotoxic and phototoxic damage in addition to the cellular response to the assault under study.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous immersion Mirau interferometry

Lyulko

Randers-Pehrson

Brenner

2013

Review of Scientific Instruments

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A novel technique for label-free imaging of live biological cells in aqueous medium that is insensitive to ambient vibrations is presented. This technique is a spin-off from previously developed immersion Mirau interferometry. Both approaches utilize a modified Mirau interferometric attachment for a microscope objective that can be used both in air and in immersion mode, when the device is submerged in cell medium and has its internal space filled with liquid. While immersion Mirau interferometry involves first capturing a series of images, the resulting images are potentially distorted by ambient vibrations. Overcoming these serial-acquisition challenges, simultaneous immersion Mirau interferometry incorporates polarizing elements into the optics to allow simultaneous acquisition of two interferograms. The system design and production are described and images produced with the developed techniques are presented.

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Digital holographic microscopy for live cell applications and technical inspection

Cited by 706 publications

References 50 publications

Quantitative Phase Imaging for Label-Free Analysis of Cancer Cells—Focus on Digital Holographic Microscopy

Quantitative Phase Imaging for Label-Free Analysis of Cancer Cells—Focus on Digital Holographic Microscopy

Principles and techniques of digital holographic microscopy

Simultaneous immersion Mirau interferometry

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