Dimensional study of prostate cancer using stereological tools

Santamarı́a, Luis; Ingelmo, Ildefonso; Teba, Fernando

doi:10.1111/joa.13524

Cited by 3 publications

(1 citation statement)

References 56 publications

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“…However, it should be stressed here that the effects of all tissue constituents must not be viewed separately, due to the fact that they are competing for their area fractions. The finding of area fractions of prostate tissue constituents in Figure 7 is consistent with the values reported in a previous study 47 . It is therefore not surprising to observe that stroma and epithelial compartments have greater effects on, and correlation with, the apparent properties, due to the fact that these two constituents have greater area fractions as confirmed by the results in Figure 7.…”

Section: Discussionsupporting

confidence: 90%

Computational homogenization of histological microstructures in human prostate tissue: Heterogeneity, anisotropy and tension‐compression asymmetry

Anderson

Ntala

Özel

et al. 2023

Numer Methods Biomed Eng

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Human prostatic tissue exhibits complex mechanical behaviour due to its multiphasic, heterogeneous nature, with hierarchical microstructures involving epithelial compartments, acinar lumens and stromal tissue all interconnected in complex networks. This study aims to establish a computational homogenization framework for quantifying the mechanical behaviour of prostate tissue, considering its multiphasic heterogeneous microstructures and the mechanical characteristics of tissue constituents. Representative tissue microstructure models were reconstructed from high‐resolution histology images. Parametric studies on the mechanical properties of the tissue constituents, particularly the fibre‐reinforced hyper‐elasticity of the stromal tissue, were carried out to investigate their effects on the apparent tissue properties. These were then benchmarked against the experimental data reported in literature. Results showed significant anisotropy, both structural and mechanical, and tension‐compression asymmetry of the apparent behaviours of the prostatic tissue. Strong correlation with the key microstructural indices such as area fractions of tissue constituents and the tissue fabric tensor was also observed. The correlation between the stromal tissue orientation and the principal directions of the apparent properties suggested an essential role of stromal tissue in determining the directions of anisotropy and the compression‐tension asymmetry characteristics in normal human prostatic tissue. This work presented a homogenization and histology‐based computational approach to characterize the apparent mechanical behaviours of human prostatic or other similar glandular tissues, with the ultimate aim of assessing how pathological conditions (e.g., prostate cancer and benign prostatic hyperplasia) could affect the tissue mechanical properties in a future study.

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Section: Discussionsupporting

confidence: 90%

Computational homogenization of histological microstructures in human prostate tissue: Heterogeneity, anisotropy and tension‐compression asymmetry

Anderson

Ntala

Özel

et al. 2023

Numer Methods Biomed Eng

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Dimensional study of prostate cancer using stereological tools

2021

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Reduced Tumor Volume and Increased Necrosis of Human Breast Tumor Xenograft in Mice Pretreated by a Cocktail of Three Specific Anti-HER2 scFvs

Nejatollahi,

Nadimi,

Noorafshan

et al. 2024

CPPS

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Purpose: We aimed to assess the effects of a cocktail comprising three specific antiHER2 scFvs on breast tumor formation in a xenograft mouse model and to evaluate quantitative changes in the tumor using stereological analysis. Methods: Three specific anti-HER2 phage antibodies were produced from a scFv-library using phage display technology. The cell binding capacities of the antibodies were assessed via FACS analysis. Soluble forms of the antibodies were prepared by infecting HB2151-E. coli cells and purified using a centrifugal ultrafiltration method. The purification process was evaluated by SDSPAGE analysis. Two forms of scFv cocktails were prepared, soluble scFv and phage-scFv cocktail, which contained an equal amount/phage of the three specific anti-HER2 antibodies. Inbred female BALB/c mice were pretreated with 5 and 20 mg/kg of the soluble scFv cocktail and 1011 phagescFv cocktail/kg. The mice were then injected with 2×106 SKBR-3 human breast cancer cells. Total tumor, inflammatory and non-inflammatory volumes were estimated using the Cavalieri principle after preparing photomicrograph slides. Results: The anti-HER2 scFvs showed significantly higher binding to SKBR-3 cells compared to the isotype control. SDS-PAGE analysis confirmed the high purification of the scFvs. Stereological analysis revealed that the group pretreated with 20 mg/kg of the soluble scFv cocktail exhibited the highest reductions in total tumor volume, non-inflammatory volume, and inflammatory volume, with reductions of 73%, 78%, and 72%, respectively, compared to PBS-pretreated mice (P-value < 0.0001). The volumetric ratio of necrotic tissue to total tumor volume increased by 2.2-fold and 2- fold in the 20mg/kg of soluble scFv cocktail and phage-scFv cocktail groups, respectively, compared to the PBS-treated mice (P-value < 0.05). Conclusion: Pre-treatment with a 20 mg/kg anti-HER2 scFv cocktail resulted in a significant reduction in tumor volume and increased necrotic area in a human breast cancer xenograft model, indicating the remarkable anti-tumor effect of the cocktail in vivo.

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Dimensional study of prostate cancer using stereological tools

Abstract: Prostate cancer remains a common carcinoma affecting the male population (Coloma et al., 2014). Little is known about the nature and variability of dimensional changes in the structure of tumor glands and their relationship with cancer behavior. All factors related

Cited by 3 publications

References 56 publications

Computational homogenization of histological microstructures in human prostate tissue: Heterogeneity, anisotropy and tension‐compression asymmetry

Computational homogenization of histological microstructures in human prostate tissue: Heterogeneity, anisotropy and tension‐compression asymmetry

Dimensional study of prostate cancer using stereological tools

Reduced Tumor Volume and Increased Necrosis of Human Breast Tumor Xenograft in Mice Pretreated by a Cocktail of Three Specific Anti-HER2 scFvs

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