Diosmin Protects Rat Retina from Ischemia/Reperfusion Injury

Tong, Nianting; Zhang, Zhenzhen; Gong, Yuanyuan; Yin, Li; Wu, Xinming

doi:10.1089/jop.2011.0218

Cited by 30 publications

(22 citation statements)

References 35 publications

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“…Studies have presumed that oxidative stress is likely to be causally involved in IR-induced retinal damage [4,5]. Oxidative stress is defined as an increase over physiological values in the intracellular concentrations of reactive oxygen species (ROS).…”

Section: Introductionmentioning

confidence: 99%

“…It seems reasonable to consider the potential use of defined antioxidants in the treatment of retinal ischemia diseases. Tong et al [5] proved that diosmin markedly protected the retina from IR-induced injury by preventing the loss of retinal neurons and improving the oxidative parameters of the tissue, and a recent study also showed that several kinds of drugs with antioxidant properties significantly counteracted the negative effects induced by retinal ischemia [12]. …”

Section: Introductionmentioning

confidence: 99%

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Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina

Chen

Yang

2015

Ophthalmic Res

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Aims: Crocin, a pharmacologically active component of Crocus sativus L. (saffron), has been reported to be useful in the treatment of oxidative stress injury. In the present study, we investigated the antioxidative effect of crocin and the change of the ERK signaling pathway on rat retina induced by ischemia/reperfusion (IR) injury. Methods: Crocin was pretreated 30 min before and once daily after retinal IR injury by intraperitoneal injection. The retinal morphological damage was observed by hematoxylin and eosin (HE) staining. The number of retinal ganglion cells (RGCs) was counted by Brn-3a immunofluorescence staining. The antiapoptotic effect of crocin was determined by detecting cleaved caspase-3 protein levels by means of Western blot and immunohistochemical analysis. Furthermore, retinas were also used for the determination of malondialdehyde (MDA) levels, glutathione (GSH) levels, total superoxide dismutase (T-SOD), and reactive oxygen species (ROS). The phosphorylated ERK (p-ERK) protein level was determined by Western blot and immunohistochemical analysis. Results: Our results showed that crocin treatment (50 mg/kg) significantly inhibited the decrease of retinal thickness through HE staining and protected RGCs from decreasing. Compared with the IR + vehicle group, crocin treatment significantly decreased cleaved caspase-3 and p-ERK protein expression by Western blot analysis and immunohistochemistry. Immunohistochemical staining for cleaved caspase-3 and p-ERK in the retinal sections showed positive cells were present in the RGC layer and inner nuclear layer after IR injury. Similarly, crocin (50 mg/kg) treatment also significantly increased the level of activity of GSH, enhanced the activity of T-SOD, and decreased the activity level of ROS and MDA after IR injury. Conclusions: These findings demonstrated that crocin treatment could notably protect the retina from damage induced by IR. It might be related to crocin antioxidant and antiapoptotic properties in the retina.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina

Chen

Yang

2015

Ophthalmic Res

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“…In our previous study [11], we found a neuroprotective effect of diosmin against retinal I/R injury. Furthermore, as a naturally occurring flavonoid glycoside, diosmin is a vascular protective agent used to treat chronic venous insufficiency [12], hemorrhoids, lymphedema, and varicose veins.…”

Section: Introductionmentioning

confidence: 75%

“…Rats were divided into two groups (n = 16/group): a physiological saline-treated group (Vehicle) and a 10 mg/kg diosmin-treated group (Diosmin). This dosage was chosen based on our previous study [11]. Animals were given physiological saline or diosmin intragastrically 30 min before the onset of ischemia, and then daily after I/R injury until sacrifice.…”

Section: Methodsmentioning

confidence: 99%

Diosmin Alleviates Retinal Edema by Protecting the Blood-Retinal Barrier and Reducing Retinal Vascular Permeability during Ischemia/Reperfusion Injury

Tong

Zhang

et al. 2013

PLoS ONE

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Background and PurposeRetinal swelling, leading to irreversible visual impairment, is an important early complication in retinal ischemia/reperfusion (I/R) injury. Diosmin, a naturally occurring flavonoid glycoside, has been shown to have antioxidative and anti-inflammatory effects against I/R injury. The present study was performed to evaluate the retinal microvascular protective effect of diosmin in a model of I/R injury.MethodsUnilateral retinal I/R was induced by increasing intraocular pressure to 110 mm Hg for 60 min followed by reperfusion. Diosmin (100 mg/kg) or vehicle solution was administered intragastrically 30 min before the onset of ischemia and then daily after I/R injury until the animals were sacrificed. Rats were evaluated for retinal functional injury by electroretinogram (ERG) just before sacrifice. Retinas were harvested for HE staining, immunohistochemistry assay, ELISA, and western blotting analysis. Evans blue (EB) extravasation was determined to assess blood–retinal barrier (BRB) disruption and the structure of tight junctions (TJ) was examined by transmission electron microscopy.ResultsDiosmin significantly ameliorated the reduction of b-wave, a-wave, and b/a ratio in ERG, alleviated retinal edema, protected the TJ structure, and reduced EB extravasation. All of these effects of diosmin were associated with increased zonular occluden-1 (ZO-1) and occludin protein expression and decreased VEGF/PEDF ratio.ConclusionsMaintenance of TJ integrity and reduced permeability of capillaries as well as improvements in retinal edema were observed with diosmin treatment, which may contribute to preservation of retinal function. This protective effect of diosmin may be at least partly attributed to its ability to regulate the VEGF/PEDF ratio.

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“…Это приводит к уменьшению высво-бождения воспалительных радикалов, простагландинов и тромбоксана. Антиоксидантные свойства, достигаются путѐм уменьшения количества свободных радикалов, оказывающих повреждающее действие на биологические мембраны, провоцируя развитие эндотелиальной дисфункции [64]. Известны следующие основные клинические эффекты микронизированной очищенной фракции флавоноидов: улучшение микроциркуляции: угнетение выработки молекул адгезии [65], препятствует адгезии лейкоцитов и / или их миграции, ингибирует образование свободных радикалов [66], макрофагов, тормозит синтез простагландина Е2 или F2 и тромбоксана В2 ингибирует увеличение микрососудистой проницаемости, индуцированной ишемией [67] и гистамин, брадикинин, лейкотриен В4 при ишемии либо реперфузии снижение апоптоза паренхиматозных клеток, ингибирует тромбоцитарную активность [68] (in vitro); лимфотропный: повышает сократимость лимфатических сосудов в лабораторных условиях [69], улучшает лимфатический дренаж [70], снижает концентрацию белка в ткани и фибробластов при лимфостазе [71].…”

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Endothelial Dysfunction in Patients With Secondary Lymphedema and Methods of Its Correction (Literature Review)

Каlinin¹,

Suchkov²,

Maksaev³

2019

HMJ

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Diosmin Protects Rat Retina from Ischemia/Reperfusion Injury

Abstract: Diosmin protected the retina from I/R injury, possibly via a mechanism involving the regulation of oxidative parameters.

Cited by 30 publications

References 35 publications

Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina

Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina

Diosmin Alleviates Retinal Edema by Protecting the Blood-Retinal Barrier and Reducing Retinal Vascular Permeability during Ischemia/Reperfusion Injury

Endothelial Dysfunction in Patients With Secondary Lymphedema and Methods of Its Correction (Literature Review)

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