Direct and protective effects of single or combined addition of vincristine and ε-viniferin on human HepG2 cellular oxidative stress markers in vitro

Abstract

The objective of this study is to examine the direct effects of low doses and high doses of e-viniferin, a substance known to be an antioxidant, and vincristine sulphate, a chemotherapeutic agent, alone and in combination [e-viniferin ? vincristine] on HepG2 cell strain, as well as evaluate oxidative stress after incubation periods of 3, 6, and 24 h. Direct effect was determined right after the incubation period; however, for protective effect, antioxidant protection response was determined after the treatment for 1 h with 500 lM H 2 O 2 , which is an oxidative stressor. For this purpose, superoxide dismutase was determined for enzyme activity, and lipid hydroperoxide (LPO) and reduced glutathione concentrations were studied as indicators of oxidative stress. Results show that low [3.63 lM vincristine ? 3.75 lM e-viniferin] and high [11.25 lM vincristine ? 15.8 lM e-viniferin] doses of combination groups showed similar direct antioxidant effect on LPO levels as protective when compared to the H 2 O 2 control group (p \ 0.05). Superoxide dismutase enzyme showed a direct antioxidant effect in low and high dose combination groups. In addition, when the incubation period was increased to 24 h, a protective effect was observed in both dose groups (p \ 0.05). Reduced glutathione activities showed a direct effect in the low dose combination group, and a protective effect in both the low and high doses in the 24 h. These results show that combined usage of drugs in HepG2 cell strain possesses a protective effect against exogenically produced oxidative stress conditions.

Beneficial Effects of ε-Viniferin on Obesity and Related Health Alterations

“…ε-viniferin is probably the most studied form of viniferin, due to its many biological activities [ 5 ], such as anti-inflammatory [ 6 ], antioxidant [ 7 ], antineoplasic [ 8 ], antiobesity [ 9 ], cardioprotective [ 10 ] and neuroprotective actions [ 11 ]. As occurred with resveratrol, it was first described in grapevine leaves by Langcake and Price in 1977, who observed that it is a resveratrol dehydrodimer synthesized via oxidative cyclization [ 12 ].…”