Direct enzymatic acylation of cellulose pretreated in BMIMCl ionic liquid

Alkyl-methylimidazolium ionic liquids affect the growth and fermentative metabolism of Clostridium sp.

“…Ionic liquid (IL) based pretreatment methods show promise for the rapid and efficient dissolution of lignocellulosic biomass and extraction of cellulose (Datta et al, 2010;Singh et al, 2009;Zavrel et al, 2009). Ionic liquids are extensively investigated for cellulose dissolution (Gremos et al, 2011;Swatloski et al, 2002;Vitz et al, 2009) and biomass deconstruction (Fort et al, 2007;Sun et al, 2011;Xie et al, 2007;Zhang et al, 2011). The regenerated cellulose from ILs was found to be amorphous, porous and more accessible to hydrolysis by cellulases (Dadi et al, 2006).…”

New findings about the lipase acetylation of nanofibrillated cellulose using acetic anhydride as acyl donor

“…The acetylation extent, achieved in an organic solvent solution, was 50 times higher than that achieved in aqueous solution, being attributed to the absence of bulk water and the increase in substrate solubility by the transition of reaction media from aqueous solution to organic solvents, as well as the ability of lipase to remain catalytically active in highly polar dimethyl sulphoxide (DMSO). Gremos, Zarafeta, Kekos, and Kolisis (2011) successfully esterified Avicel cellulose with vinyl propionate, vinyl laurate and vinyl stearate within a solvent-free reaction system using various hydrolases. However, ionic liquid BMIMCl (1-n-butyl-3-methylimidazolium chloride) pre-treatment was necessary in order to facilitate the unwrapping of the structure of the polysaccharide molecule and make it accessible to the enzyme.…”

Enhancement of acetyl xylan esterase activity on cellulose acetate through fusion to a family 3 cellulose binding module

“…Enzymes have also been used to directly modify the surface chemistry of cellulose microfibrils. For example, lipases can promote stereo and regioselective acylation and deacylation of carbohydrates, including cellulose [7][8][9][10][11][12][13].…”