Direct HPLC Analysis of Trimethoprim in Milk

Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis

“…The ADS column can tolerate up to 80-100 ml of plasma [50,53,54]. The LiChrospher ADS-RP was able to achieve the direct analysis of pharmaceutical compounds in several biological matrices such as plasma [49,50,53,[55][56][57][58][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73], serum [42,43,[74][75][76][77][78][79], urine [60,75,76,78,80], microdialysate [81,82], saliva [64], liver homogenate [49], intestinal aspirates [76], cell cultures [68,83], bronchial secretions [74], milk [84] and tissue [67].…”

High-performance liquid chromatography method for the simultaneous determination of sulfamethoxazole and trimethoprim in bovine milk using an on-line clean-up column

“…This type of packing has shown excellent efficiency for protein exclusion of human plasma [15,18], but analysis of drugs in milk without tedious and time consuming pre-treatment procedures remains a challenge. Column-switching techniques have been widely used for on line clean-up of biological samples but the number of methods employing RAM columns for analysis of exogenous compounds in milk samples is, however, limited [10,16,[19][20][21][22].…”

“…A number of analytical HPLC methods have been published which describe the analysis of sulfonamides [5][6][7][8][9] and trimethoprim [10] in milk. Because some of these methods are labor intensive, requiring extensive sample pre-treatments, they cannot be used for routine analysis of numerous samples.…”

The development of selective and biocompatible coatings for solid phase microextraction

“…RAMs have been applied to the analysis of foods [88], drugs in biological fluids [89 -91], including chiral analysis [80,92,93], and flow based immunoassay for the separation of free and bound labels [94,95]. An online multidimensional solid phase extraction utilizing the selectivity of MIPs has been performed by the group of Boos [96 -98] and the performance of RAMs has been compared to traditional off-line SPE [99].…”