Directed differentiation of induced pluripotent stem cells into chondrogenic lineages for articular cartilage treatment

Lach, Michał Stefan; Trzeciak, Tomasz; Richter, Magdalena; Pawlicz, Jarosław; Suchorska, Wiktoria Maria

doi:10.1177/2041731414552701

Cited by 21 publications

(15 citation statements)

References 110 publications

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“…Pluripotent SCs are able to self-renew unrestrictedly. They can differentiate in vitro and in vivo in all cell types deriving from the three germ layers ( 1 , 2 ). This ability makes them useful in cell replacement therapy and the treatment of numerous diseases ( 3 ), including diabetes ( 4 ), neurodegenerative ( 5 ), retinal ( 6 ) and cardiac ( 7 ) diseases, as well as muscular dystrophy ( 8 ).…”

Section: Stem Cells: Genetic Integritymentioning

confidence: 99%

Genetic stability of pluripotent stem cells during anti-cancer therapies

Suchorska

Augustyniak

Łukjanow

2016

Experimental and Therapeutic Medicine

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Regenerative medicine is a rapidly growing field that holds promise for the treatment of many currently unresponsive diseases. Stem cells (SCs) are undifferentiated cells with long-term self-renewal potential and the capacity to develop into specialized cells. SC-based therapies constitute a novel and promising concept in regenerative medicine. Radiotherapy is the most frequently used method in the adjuvant treatment of tumorous alterations. In the future, the usage of SCs in regenerative medicine will be affected by their regular and inevitable exposure to ionizing radiation (IR). This phenomenon will be observed during treatment as well as diagnosis. The issue of the genetic stability of SCs and cells differentiated from SCs is crucial in the context of the application of these cells in clinical practice. This review examines current knowledge concerning the DNA repair mechanisms (base excision repair, nucleotide excision repair, mismatch repair, homologous recombination and non-homologous end-joining) of SCs in response to the harmful effects of genotoxic agents such as IR and chemotherapeutics.

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Section: Stem Cells: Genetic Integritymentioning

confidence: 99%

Genetic stability of pluripotent stem cells during anti-cancer therapies

Suchorska

Augustyniak

Łukjanow

2016

Experimental and Therapeutic Medicine

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“…Induced pluripotent stem cells (iPSCs) are reprogrammed somatic cells which resemble embryonic stem cells and can be differentiated into numerous types of cells [6–9]. Therefore, human iPSCs (hiPSCs) are considered to be a potential resource for restoring NP cells and disc homeostasis.…”

Section: Introductionmentioning

confidence: 99%

Intradiscal Injection of Induced Pluripotent Stem Cell-Derived Nucleus Pulposus-Like Cell-Seeded Polymeric Microspheres Promotes Rat Disc Regeneration

Xia

Zhu

Hua

et al. 2019

Stem Cells International

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Background. Cell replacement therapy is an attractive alternative for treating degenerated intervertebral discs (IVDs), which are related to the reduction of nucleus pulposus-like cells (NP-lCs) and the loss of the extracellular matrix. Induced pluripotent stem cells (iPSCs) which resemble embryonic stem cells are considered to be a potential resource for restoring NP-lCs and disc homeostasis. Here, we proposed an efficient two-step differentiation protocol of human iPSCs into NP-lCs and continuously tested their in vivo ability to regenerate IVDs. Methods. A polymeric gelatin microsphere (GM) was generated for sustained release of growth and differentiation factor-5 (GDF-5) and as a cell delivery vehicle of NP-lCs. By injecting NP-lC-seeded GDF-5-loaded GMs into the rat coccygeal intervertebral discs, the disc height and water content were examined with the molybdenum target radiographic imaging test and magnetic resonance imaging examination. Histology and immunohistochemistry results were shown with H&E, S-O-Fast Green, and immunohistochemistry staining. Results. We demonstrated that the injection of NP-lC-seeded GDF-5-loaded GMs could reverse IDD in a rat model. The imaging examination indicated that disc height recovered and water content increased. Histology and immunohistochemistry results indicated that the NP cells as well as their extracellular matrix were partially restored. Conclusions. The results suggest that NP-lC-seeded GDF-5-loaded GMs could partially regenerate degenerated intervertebral discs after transplantation into rat coccygeal intervertebral discs. Our study will help develop a promising method of stem cell-based therapy for IDD.

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“…One of the potential solutions to that state is taking an advantage of the recent discoveries of tissue engineering [7,8]. The application of mesenchymal stromal cells (MSC) is studied very broadly since chondrocytes are originating from the mesoderm germ layer.…”

Section: Introductionmentioning

confidence: 99%

The effect of the combination of TGF-β1 and BMP2 with high-density pellet cell culture during chondrogenic differentiation of pluripotent stem cells.

Lach

Suchorska

2020

LOS

Self Cite

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Introduction: The osteoarthritis is a serious threat for well-developed and ageing countries. Present techniques of treatment of damaged cartilage are not sufficient. Hence, new strategies should be developed. One of the potential sources for the regeneration of cartilage is pluripotent stem cells (PSC). Aim: The development of an efficient protocol of chondrogenic differentiation using PSC. Material and methods: The human embryonic stem cell line (BG01V) was used in this study. The chondrogenic differentiation was performed using high-density pellet culture in the presence of TGF-β1 (10 ng/ml) and BMP2 (100 ng/ml). After 21 days gene expression analysis of markers related to chondrogenesis was done. Additionally, the histological staining was performed to detect the deposition of proteoglycans and collagens in differentiated pellet culture. Results: Obtained pellets exhibited decreased expression of pluripotent markers. The upregulation of mesodermal marker and type II collagen was observed in differentiated pellets in the presence of applied growth factors. The histological analysis revealed mild deposition of proteoglycans and collagens. Conclusion: The presented approach enables to obtain chondrogenic pellets in their early stages of chondrogenesis.

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Directed differentiation of induced pluripotent stem cells into chondrogenic lineages for articular cartilage treatment

Cited by 21 publications

References 110 publications

Genetic stability of pluripotent stem cells during anti-cancer therapies

Genetic stability of pluripotent stem cells during anti-cancer therapies

Intradiscal Injection of Induced Pluripotent Stem Cell-Derived Nucleus Pulposus-Like Cell-Seeded Polymeric Microspheres Promotes Rat Disc Regeneration

The effect of the combination of TGF-β1 and BMP2 with high-density pellet cell culture during chondrogenic differentiation of pluripotent stem cells.

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