Disassembling the complexity of mucus barriers to develop a fast screening tool for early drug discovery

Pacheco, Daniela Peneda; Butnarasu, Cosmin; Briatico‐Vangosa, Francesco; Pastorino, Laura; Visai, Livia; Visentin, Sonja; Petrini, Paola

doi:10.1039/c9tb00957d

Cited by 34 publications

(50 citation statements)

References 66 publications

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“…Sodium alginate (SA) is biodegradable, biocompatible and non-toxic, and is obtained from brown algae. Because of its structural similarities to the extracellular matrix, and its gelling properties under conditions compatible with biological activities, it is widely used in biomedical engineering applications, for example, drug delivery, in vitro cell culture, wound healing, and tissue engineering [ 31 , 32 , 33 ]. Electrospun alginate-based nanofibers have been developed for use in biomedical applications such as wound dressing [ 34 , 35 , 36 ], tissue engineering [ 37 , 38 ], and drug delivery systems [ 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

Green Approach to Develop Bee Pollen-Loaded Alginate Based Nanofibrous Mat

Pakolpakçıl

Draczyński

2021

Materials

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Green electrospun materials are gaining popularity in the quest for a more sustainable environment for human life. Bee pollen (BP) is a valuable apitherapeutic product and has many beneficial features such as antioxidant and antibacterial properties. Alginate is a natural and low-cost polymer. Both natural materials show good compatibility with human tissues for biomedical applications and have no toxic effect on the environment. In this study, bee pollen-loaded sodium alginate and polyvinyl alcohol (SA/PVA) nanofibrous mats were fabricated by the electrospinning technique. The green electrospun nanofibrous mats were analyzed by scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FTIR), and differential scanning calorimeter (DSC). According to the findings of the study, the toxin-free electrospinning method is suitable for producing green nanomaterial. Because of the useful properties of the bee pollen and the favorable biocompatibility of the alginate fibers, the bee pollen-loaded SA/PVA electrospun mats have the potential for use in a variety of biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Green Approach to Develop Bee Pollen-Loaded Alginate Based Nanofibrous Mat

Pakolpakçıl

Draczyński

2021

Materials

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“…Despite the turbidity curves in undiluted conditions, TEM analysis showed a significant reduction in fibril diameter only for COLL-HA hydrogels, suggesting structural changes in the polymer network and a possible influence on biological performances. 36 Starting from the fitting of rheological data, the Generalized Maxwell Model 22 estimated that the mesh size decreased in this order: COLL-HA (116 nm) > COLL-PEG 2000 (80 nm) > COLL-PEG 3350 (75 nm). Since for similar concentrations of type I COLL, thin fibrils form networks with smaller pore sizes than thick fibers, 37 the trends indicated by the Generalized Maxwell Model and TEM analysis agree.…”

Section: Discussionmentioning

confidence: 99%

“…We estimated hydrogel permeability from the mesh size. We calculated this parameter by fitting the rheological data 15 with the Generalized Maxwell Model, 22 obtaining a value of 80 nm. We estimated a mesh size of 116 nm for COLL-HA and a mesh size of 75 nm for COLL-PEG 3350 gels.…”

Section: Effect Of Hydrogel Thicknessmentioning

confidence: 99%

A miniaturized hydrogel-based in vitro model for dynamic culturing of human cells overexpressing beta-amyloid precursor protein

et al. 2020

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Recent findings have highlighted an interconnection between intestinal microbiota and the brain, referred to as microbiota–gut–brain axis, and suggested that alterations in microbiota composition might affect brain functioning, also in Alzheimer’s disease. To investigate microbiota–gut–brain axis biochemical pathways, in this work we developed an innovative device to be used as modular unit in an engineered multi-organ-on-a-chip platform recapitulating in vitro the main players of the microbiota–gut–brain axis, and an innovative three-dimensional model of brain cells based on collagen/hyaluronic acid or collagen/poly(ethylene glycol) semi-interpenetrating polymer networks and β-amyloid precursor protein-Swedish mutant-expressing H4 cells, to simulate the pathological scenario of Alzheimer’s disease. We set up the culturing conditions, assessed cell response, scaled down the three-dimensional models to be hosted in the organ-on-a-chip device, and cultured them both in static and in dynamic conditions. The results suggest that the device and three-dimensional models are exploitable for advanced engineered models representing brain features also in Alzheimer’s disease scenario.

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“…There is not "the ideal" in vitro model of lung microbiota, as it is strictly dependent on the feature to be tested. In antibiotic resistance studies, e.g., the 3D-matrix is relevant for permeability studies (Pacheco et al, 2019) but also to allow the formation of self-protecting clusters of bacteria (Melaugh et al, 2016). We propose a bottom-up approach in recapitulating the physiological complexity of the microbial niches within in vitro models.…”

Section: In Vitro Modeling the Three Dimensional And Multimicrobial Communitiesmentioning

confidence: 99%

The Open Challenge of in vitro Modeling Complex and Multi-Microbial Communities in Three-Dimensional Niches

Oriano

Zorzetto

Guagliano

et al. 2020

Front. Bioeng. Biotechnol.

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Disassembling the complexity of mucus barriers to develop a fast screening tool for early drug discovery

Abstract: Mucus is a natural barrier with a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites.

Cited by 34 publications

References 66 publications

Green Approach to Develop Bee Pollen-Loaded Alginate Based Nanofibrous Mat

Green Approach to Develop Bee Pollen-Loaded Alginate Based Nanofibrous Mat

A miniaturized hydrogel-based in vitro model for dynamic culturing of human cells overexpressing beta-amyloid precursor protein

The Open Challenge of in vitro Modeling Complex and Multi-Microbial Communities in Three-Dimensional Niches

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