Discrepancies in Analyses of Deoxyribonucleic Acid in Rabbit Spermatozoa, Involving Feulgen Staining (Feulgendna) and Ultraviolet Light Absorption (Uv-Dna) Measurements

Bouters, R.; Esnault, Charles; Salisbury, G. W.; Ortavant, R.

doi:10.1530/jrf.0.0140355

Cited by 25 publications

(8 citation statements)

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“…These changes are distinct from, if additional to, presumed deterioration of the acrosome which will affect sperm penetration and, if widespread, will ultimately cause total failure offertilization. Ageing of bull spermatozoa in vitro has been shown to bring about a change in the reaction of the spermatozoa to Feulgen staining and to influence prenatal losses (Salisbury, Birge, de la Torre & Lodge, 1961 ;Salisbury, 1967) and there is evidence also that, in the rabbit, ageing in vivo of epididymal spermatozoa influences the DNA content of the spermatozoa (Bouters, Esnault, Salisbury & Ortavant, 1967). There is, thus, considerable support for the notion that the ageing of spermatozoa affects their DNA content.…”

Section: Discussionmentioning

confidence: 98%

Ageing of Rabbit Spermatozoa in the Male Tract and Its Effect on Fertility

Tesh¹,

Glover²

1969

Reproduction

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The influence on fertility of the ageing of rabbit spermatozoa in the male tract has been investigated. To age the spermatozoa, the corpus epididymidis was ligated bilaterally so that the inflow of younger spermatozoa into the tail of the epididymis from higher levels of the duct was prevented. Ejaculated semen samples collected from the rabbits at different times after the operation were used for insemination. It was found that the fertilizing capacity of the spermatozoa was lost after 49 days. Losses before implantation increased progressively until 28-day\x=req-\ old spermatozoa were used, but post-implantation losses reached a peak with 35-day-old spermatozoa. After implantation, losses appeared to occur at progressively earlier stages of development as the age of the spermatozoa increased. In the strain ofrabbits used, several foetuses were seen to lack gall bladders and to have abnormal skull sutures following the insemination of does with aged spermatozoa.Throughout the experiment, the motility, morphology and reaction ofthe spermatozoa to staining with aqueous nigrosin\p=m-\eosin was examined. The significance and possible causes of adverse changes in ageing spermatozoa are discussed. INTRODUCTIONThe tail of the epididymis is a storage area for mature spermatozoa (Von Lanz

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Section: Discussionmentioning

confidence: 98%

Ageing of Rabbit Spermatozoa in the Male Tract and Its Effect on Fertility

Tesh¹,

Glover²

1969

Reproduction

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“…In the presence of vas deferens obstruction, however, sperm cells may survive for many weeks (8), creating in the epididymis a highly heterogeneous environment in terms of spermatozoa age and maturity. Because there is no known correlation between physical appearance or degree of motility of the epididymal sperm cell and the level of its deterioration (9), a sperm selected for ICSI could be one that was present in the epididymis for 1 day or several weeks.…”

mentioning

confidence: 99%

Embryos generated using testicular spermatozoa have higher developmental potential than those obtained using epididymal spermatozoa in men with obstructive azoospermia

Dozortsev

Neme

Diamond

et al. 2006

Fertility and Sterility

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“…It has been shown that the Feulgen-DNA contents of ejaculated spermatozoa are lower than those of epididymal or testicular spermatozoa (Bouters, Esnault, Salisbury & Ortavant, 1967). In the present study, all the spermatozoa were taken from the same region of the genital tract and were processed for the Feulgen reaction by methods prescribed to eliminate proportionality errors (Böhm et al, 1968).…”

mentioning

confidence: 84%