In this study we present a simulation based comparative performance analysis between OSPF and EIGRP for real time applications by using Optimized Network Engineering Tools (OPNET) and Graphical Network Simulator (GNS3). In order to evaluate OSPF and EIGRP's performance, we have designed three network models where the first, second and third Network model are configured, respectively with OSPF, EIGRP and combination of EIGRP and OSPF. This study shows the estimation of proposed routing protocols based on the quantitative metrics such as Convergence Time, Throughput, End to End delay, Jitter and Packet Loss through the simulated network models. The evaluation results show that EIGRP routing protocol provides a better performance than OSPF routing protocol for real time applications.