Discrimination among 136 Tetraploid Potato Varieties by Fingerprints Using Highly Polymorphic DNA Markers

Abstract

The identification of a variety has an important role in the protection of plant breeders' rights and for monitoring seed production and marketing. The objective of our study was to examine whether or not a large number of registered cultivars of European potato (Solarium tuberosum subsp, tuberosum) could be distinguished using RFLPs (restriction fragment length polymorphisms) as genetic fingerprints. Genomic Southern blots with TaqI‐restricted DNA of 136 tetraploid potato varieties were hybridized to highly polymorphic potato RFLP markers. The use of four markers was sufficient to identify 130 of 136 varieties. Three pairs of varieties were identical with all markers evaluated. One marker, GP35, was the most effective: out of 134 varieties analyzed with this probe, 122 had unique RFLP patterns. The remaining 12 varieties were divided into six pairs, each showing an identical pattern. Sequence analysis showed that GP35 was not homologous to minisatellite sequences. The probability of finding a random match of RFLP patterns between two tetraploid varieties with marker GP35 was estimated to be 0.63 × 10−4. Variety identification by RFLP fingerprints is therefore feasible in potato.

Molecular characterization of potato (Solanum tuberosum L.) genotypes using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers

“…To complement these methods and address some of the limitations, molecular markers have been developed, among which there are those based on hybridization and PCR methods (Semagn, 2006). RFLP is a molecular marker system based on hybridization and has been used extensively to construct genetic maps (Bonierbale et al, 1988), conduct phylogenetic and diversity studies in potato (Görg et al, 1992;Ritter, 2000). Nevertheless, it is time consuming, expensive and requiring high level technical support (Karp et al, 1997).…”

Oryzalin Treatment of Potato Diploids Yields Tetraploid and Chimeric Plants from which Euploids could be Derived by Callus Induction

“…Görg et al (1992) used RFLP markers to discriminate potato cultivars on the German variety list. Ghislain et al (1999) analyzed intraspecific variation of S. phureja by random amplified polymorphic DNA (RAPD) markers.…”

Linkage analysis in tetraploid potato and association of markers with quantitative resistance to late blight (Phytophthora infestans)

“…Using diploids, linkage maps based on RFLP, RAPD, and AFLP markers have been constructed (Bonierbale et al 1988;Gebhardt et al 1991;Jacobs et al 1995), and these have been used to map major genes , Van Eck et al 1993 and the genetic components of polygenic characters (Bonierbale et al 1994;Leonards-Schippers et al 1994). Molecular markers have also been used to identify and select speci®c genotypes for genetic studies (Meyer et al 1993), and for genotyping diploid and tetraploid clones (Gorg et al 1992;Demecke et al 1993).…”